Changes in Glutathione and its Associated Enzymes During Carrageenan-Induced Acute Inflammation in Mice

Toxicity and Protective Effect of Phenolic‐Enriched Ethylacetate Fraction of Ocimum gratissimum (Linn.) Leaf against Acute Inflammation and Oxidative Stress in Rats

“…Glutathione levels increase in response to carrageenan, acting as an antioxidant that reacts with free radicals and yield thiol radicals. This reaction causes depletion of GSH levels over time in animals challenged with an inflammatory response (Sin et al, ; Conner and Grisham, ).…”

“…Nitrite in pouch exudates was measured spectrophotometric methods with Griess reagent. Nonenzymic antioxidant marker, reduced gluthathione (GSH) levels in pouch exudates were determined using Ellman's reagent (Sin et al,). The index of lipid peroxidation measured as the thiobarbituric reacting substance (TBARS) via the method described by Nagababu et al () and total protein content by the Biuret method (Gornall et al, ).…”

Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats

“…Glutathione level is known to increase as a response to carrageenan injection, as it is a non-enzymatic antioxidant that reacts with free radicals and yields thiol radicals. This reaction causes depletion of the GSH level with time in animals under inflammatory assault [25,43]. In this experiment, pretreatment with MEOg prevented the depletion of GSH, which was significantly decreased by carrageenan injection in the air pouch.…”

“…It is possible that MEOg targets the migration and activation of neutrophils, as evidenced by the reduction in the myeloperoxidase activity. It has been pointed out that over 96% of the total leucocytes in carrageenan-induced acute inflammation are primarily of the polymorphonuclear cells [25]. Activated neutrophils are present in synovial fluids of patients with rheumatoid arthritis, wherein the progression of inflammation and joint destruction is associated with pronounced released neutrophilic ROS [38].…”

“…Ellman's reagent used in a modified method was employed in determining the amount of reduced glutathione (GSH) in pouch exudates [25]. Briefly, 0.1 mL cell-free exudate supernatant was diluted 10 times and deproteninzed with 1 mL Trichloroacetic acid (20%); the mixture was centrifuged at 10,000 rpm at 4 °C for 10 min.…”