Changes in Expression of Heat Shock Proteins in &lt;I&gt;Tribolium castaneum&lt;/I&gt; (Coleoptera: Tenebrionidae) in Relation to Developmental Stage, Exposure Time, and Temperature

Mahroof, Rizana; Zhu, Kun Yan; Subramanyam, Bhadriraju

doi:10.1603/0013-8746(2005)098[0100:cieohs]2.0.co;2

Cited by 66 publications

(41 citation statements)

References 36 publications

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“…Furthermore, gene expression profiles for induced HSP70s also varied after heat or cold shock during development. Mahroof et al (2005) suggested that increased mRNA abundance induced under thermal stress during different developmental stages may contribute to the increased thermotolerance in those stages. Increased thermotolerance in young larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) was suggested to result from increased expression of HSP70s (Mahroof et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Mahroof et al (2005) suggested that increased mRNA abundance induced under thermal stress during different developmental stages may contribute to the increased thermotolerance in those stages. Increased thermotolerance in young larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) was suggested to result from increased expression of HSP70s (Mahroof et al 2005). In X. c-nigrum, higher Xc-HSC70 and Xc-HSP70 expression were observed in heat and cold shocked young larvae and pupae, consistent with high heat resistance in larvae and stronger cold tolerance in young larvae and pupae.…”

Section: Discussionmentioning

confidence: 99%

“…Many types of HSP70 genes are up-regulated in response to heat or cold stress in various insects, such as Diptera (Huang & Kang 2007;Huang et al 2009;Gray 2013), Lepidoptera (Jiang et al 2012;Choi et al 2014;Shen et al 2014), and Coleoptera (Mahroof et al 2005). Because of the important roles of HSP70s in thermal stress, it is important to investigate the function and expression characteristics of HSP70 genes under temperature stress.…”

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Mamestra brassicae Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From Xestia c-nigrum granulovirus in Larvae of Mamestra brassicae and Helicoverpa armigera (Lepidoptera: Noctuidae) on Cabbage

Mukawa

Goto

2010

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Mamestra brassicae Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From Xestia c-nigrum granulovirus in Larvae of Mamestra brassicae and Helicoverpa armigera (Lepidoptera: Noctuidae) on Cabbage

Mukawa

Goto

2010

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“…As respostas diferenciadas entre os estágios de desenvolvimentos dos insetos-praga de produtos armazenados para os fumigantes também podem estar relacionadas à produção de proteínas de defesa (Mahroof et al, 2003;. Algumas proteínas, como chaperonas, estão relacionadas com a proteção celular sob condições de estresse (Ojima et al, 2005).…”

Section: Resultsunclassified

Toxicidade da combinação de dióxido de carbono e fosfina sob diferentes temperaturas para Tribolium castaneum

Aguiar¹,

Faroni²,

Guedes³

et al. 2010

Rev. bras. eng. agríc. ambient.

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RESUMOO objetivo deste trabalho foi avaliar o efeito da temperatura sobre a toxicidade da combinação de dióxido de carbono e fosfina, para os estágios de desenvolvimento de Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). A toxicidade da combinação de 5% de dióxido de carbono e 1 g m -3 de fosfina para os estágios de ovo, larvas de 5, 10 e 15 dias, pupa e adulto de T. castaneum, foi estudada nas temperaturas de 25, 30, 35, 40 e 45°C, por meio de estimativas dos tempos de exposição letais para 50 e 95% dos insetos (TL 50 e TL 95 ). Curvas tempo-resposta foram estabelecidas mediante bioensaios com períodos crescentes de exposição à combinação do dióxido de carbono com a fosfina. Observou-se que os TL 50 e TL 95 reduziram com a elevação da temperatura em todos os estágios de T. castaneum avaliados. O estágio de larva de cinco dias foi a mais susceptível à combinação de dióxido de carbono e fosfina. De acordo com os resultados, a combinação do dióxido de carbono com a fosfina é alternativa potencial para diminuir a quantidade de fosfina aplicada em produtos armazenados, por apresentar alta toxicidade para todos os estágios de T. castaneum sob diferentes temperaturas.Palavras-chave: armazenamento, fumigação, atmosfera modificada, inseto-praga Toxicity of the carbon dioxide and phosphine combination to Tribolium castaneum under different temperatures ABSTRACTThe objective of this work was to assess the effect of temperature on the toxicity of the carbon dioxide-phosphine combination for the developmental stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The toxicity of combination of 5% carbon dioxide and 1 g m -3 phosphine in the developmental stages of egg, larvae of 5, 10 and 15 days, pupae and adult of T. castaneum was studied under the temperatures of 25, 30, 35, 40 and 45°C, through the estimation of lethal insect exposure times of 50 and 95% (LT 50 and LT 95 ). For that, time-response curves were established through bioassays with increasing periods of exposure to the combination of carbon dioxide and phosphine. A reduction of LT 50 and LT 95 was observed with temperature increase in all developmental stages of T. castaneum evaluated. The 5-day old larva was the developmental stage most susceptible to the combination of carbon dioxide and phosphine. According to the results, the combination of carbon dioxide with phosphine is a potential alternative to reduce the amount of phosphine applied on stored products, due to high toxicity in all developmental stages of T. castaneum under different temperatures.

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“…The primers used for Szhsp90 were 5ʹ-CGAAGCACAA-CGATGATGAA-3ʹ and 5ʹ-TTGGGAATGTTTCTTGACGA-3ʹ, with a 182 bp product and the primers used for Szhsc70 were 5ʹ-TGCCGTACAAGCTGATATGAAA-3ʹ and 5ʹ-GACGTTGT-G A A T C ATTAAAATAGGCT-3ʹ, with a 224 bp product. The elongation factor 1-α gene (EF-1) of S. zeamais (Genheat shock, cold shock, toxic substances, pathogen infection and radiation (Mahroof et al, 2003(Mahroof et al, , 2005Guedes et al, 2008;Chen et al, 2014). Hsc70 is constitutively expressed in non-stressed cells and only mildly induced by stress (Feder & Hofmann, 1999).…”

Section: Quantifi Cation Of Gene Expressionmentioning

confidence: 99%

Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)

Tungjitwitayakul¹,

Tatun²,

Vajarasathira³

et al. 2016

Eur. J. Entomol.

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Abstract. The maize weevil, Sitophilus zeamais (Motschulsky), is a major pest of stored grain kernels. Irradiation is an established technique for controlling insects in stored grain and is a major stress factor affecting these insects. Since heat shock protein (hsp) genes respond to this stress, we proposed that hsps may be associated with irradiated stress tolerance in S. zeamais. The responses of the maize weevil to exposure to ultraviolet-C (UV-C) and microwave irradiation were assessed at four developmental stages: egg, larva, pupa and adult. The results revealed that exposure to UV-C (254 nm, < 8 h) did not affect the survival of the maize weevils; however, Szhsp70, Szhsc70 and Szhsp90 mRNA levels signifi cantly increased during the fi rst 1 h of UV-C exposure. The median lethal time (LT 50 ) of exposure to microwave irradiation indicated that the adult stage was more tolerant of microwave irradiation than the other developmental stages. Microwave irradiation enhanced the expression of the three hsps, but the intensity of up-regulation differed among the three genes, with Szhsp70 the most highly up-regulated. Our experiments revealed that UV-C and microwave irradiation infl uenced the expression profi le of hsp genes in S. zeamais. At the tissue level, the gene responses to UV-C and microwave irradiation varied greatly in different tissues.

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Changes in Expression of Heat Shock Proteins in <I>Tribolium castaneum</I> (Coleoptera: Tenebrionidae) in Relation to Developmental Stage, Exposure Time, and Temperature

Cited by 66 publications

References 36 publications

<I>Mamestra brassicae</I> Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From <I>Xestia c-nigrum granulovirus</I> in Larvae of <I>Mamestra brassicae</I> and <I>Helicoverpa armigera</I> (Lepidoptera: Noctuidae) on Cabbage

<I>Mamestra brassicae</I> Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From <I>Xestia c-nigrum granulovirus</I> in Larvae of <I>Mamestra brassicae</I> and <I>Helicoverpa armigera</I> (Lepidoptera: Noctuidae) on Cabbage

Toxicidade da combinação de dióxido de carbono e fosfina sob diferentes temperaturas para Tribolium castaneum

Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)

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