&lt;I&gt;Mamestra brassicae&lt;/I&gt; Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From &lt;I&gt;Xestia c-nigrum granulovirus&lt;/I&gt; in Larvae of &lt;I&gt;Mamestra brassicae&lt;/I&gt; and &lt;I&gt;Helicoverpa armigera&lt;/I&gt; (Lepidoptera: Noctuidae) on Cabbage

Mukawa, Shigeyuki; Goto, Chie

doi:10.1603/ec09211

Cited by 8 publications

(22 citation statements)

References 46 publications

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“…The area of cabbage leaf consumed per larva of A. nigrisigna was compared with those for M. brassicae and H. armigera reported in Mukawa and Goto (2010) using Welch's ANOVA for unequal variance.…”

Section: Discussionmentioning

confidence: 99%

“…Kinkei 201; Sakata Seed, Yokohama, Japan) was cultivated as described by Mukawa and Goto (2010). In all experiments, cabbage plants with 3-4 fully expanded true leaves were used at 28-29 days after sowing.…”

Section: Insects Plants Virus and Additivesmentioning

confidence: 99%

“…Larvae of A. nigrisigna were inoculated with a combination of MabrNPV and GVPs by feeding them virusapplied cabbage plants, as described by Mukawa and Goto (2010). In brief, 12 first-instar larvae at the molting stage, identified by head capsule slippage, were placed in a sealed container consisting of two large clear plastic cups (126 9 126 mm, 91 mm high) with the MabrNPV-applied cabbage.…”

Section: Bioassay With Cabbage Plantsmentioning

confidence: 99%

“…When larvae died without typical viral symptoms, tissue smears of the larva were prepared and examined under a phasecontrast microscope to check for the presence of the OBs. The area of cabbage leaf consumed by a second instar larva for 60 h was quantified as described by Mukawa and Goto (2010). Larvae were individually fed cabbage leaf (mean area 34.6 cm 2 , ranging from 28.6 to 46.0 cm 2 ) treated with a 0.03% spreader solution in a small plastic cap for 60 h. The feeding area of both sides of the leaf was calculated using Ryushikaiseki III image-analysis software (SMT-Kashima, Ibaraki, Japan).…”

Section: Bioassay With Cabbage Plantsmentioning

confidence: 99%

“…Our previous studies using an artificial diet bioassay have shown that MabrNPV T has pathogenicity against three noctuid pests: M. brassicae, H. armigera, and A. nigrisigna (Mukawa and Goto 2007;Goto et al 2009). In addition, we have revealed that a combination of MabrNPV T with proteins derived from Xestia c-nigrum granulovirus (XecnGV; betabaculovirus) capsules (GVPs) improves the insecticidal activity of MabrNPV against M. brassicae and H. armigera on cabbage plants (Mukawa and Goto 2010).…”

Section: Introductionmentioning

confidence: 99%

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Enhancing effect of proteins derived from Xestia c-nigrum granulovirus on Mamestra brassicae nucleopolyhedrovirus infection in larvae of Autographa nigrisigna (Lepidoptera: Noctuidae) on cabbage

Mukawa

Goto

2010

Appl Entomol Zool

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The insecticidal effect of Mamestra brassicae nucleopolyhedrovirus (MabrNPV) and the enhancing activity of proteins derived from occlusion bodies (OBs) of Xestia c-nigrum granulovirus (GVPs) on the infectivity of MabrNPV were evaluated in a bioassay with second-instar larvae of Autographa nigrisigna (Walker) fed virus-applied cabbage plants. The lethal concentrations of MabrNPV achieving 50 and 95% mortality for A. nigrisigna were estimated to be 1.4 9 10 5 and 3.1 9 10 6 OBs/ml, respectively. When larvae were fed cabbage plants treated with MabrNPV and various concentrations of GVPs, the requisite concentration of GVPs achieving 95% mortality of A. nigrisigna was estimated to be 26.2-138.8 lg/ml in combination with 10 4 OBs/ml MabrNPV and 8.46-24.09 lg/ml with 10 5 OBs/ml MabrNPV. Increases in the concentration of MabrNPV or GVPs caused larval death at younger instars. A. nigrisigna has lower susceptibility to MabrNPV than M. brassicae and Helicoverpa armigera reported in Mukawa and Goto (J Econ Entomol 103:257-264, 2010). We estimated that the requisite concentration of MabrNPV for the control of A. nigrisigna was 10 5 OBs/ml, which is a tenfold higher concentration than that for M. brassicae and H. armigera, with the increase achieved by adding 10 lg/ml GVPs.

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Section: Discussionmentioning

confidence: 99%

Section: Insects Plants Virus and Additivesmentioning

confidence: 99%

Section: Bioassay With Cabbage Plantsmentioning

confidence: 99%

Section: Bioassay With Cabbage Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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