Changes in DNA base sequences in the mutant of Arabidopsis thaliana induced by low-energy N+ implantation

Abstract

To reveal the mutation effect of low-energy ion implantation on Ambidopsis thaliana in vivo, T80II, a stable dwarf mutant, derived from the seeds irradiated by 30 keV N(+) with the dose of 80 X 10(15) ions/cm(2) was used for Random Amplified Polymorphic DNA (RAPD) and base sequence analysis. The results indicated that among total 397 RAPD bands observed, 52 bands in T80II were different from those of wild type showing a variation frequency 13.1%. In comparison with the sequences of A. thaliana in GenBank, the RAPD fragments in T80II were changed greatly in base sequences with an average rate of one base change per 16.8 bases. The types of base changes included base transition, transversion, deletion and insertion. Among the 275 base changes detected, single base substitutions (97.09%) occurred more frequently than base deletions and insertions (2.91%). And the frequency of base transitions (66.55%) was higher than that of base transversions (30.55%). Adenine, thymine, guanine or cytosine could be replaced by any of other three bases in cloned DNA fragments in T80II. It seems that thymine was more sensitive to the irradiation than other bases. The flanking sequences of the base changes in RAPD fragments in T80II were analyzed and the mutational "hotspot" induced by low-energy ion implantation was discussed.

SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

“…(Yu et al, 2008), have been carried out using RAPD markers. However, with the exception of studies in Nicotiana tabacum (Zhang et al, 1998) and Arabidopsis thaliana (Chang et al, 2003;Li et al, 2007), few investigations have focused on the base sequence changes induced by low-energy ion implantation in higher plants.…”

Bioeffects of Low Energy Ion Beam Implantation: DNA Damage, Mutation and Gene Transter

“…Their results indicated that among the three hundred and ninety seven RAPD bands observed, 52 bands in T80II were different from those of wild type showing a variation frequency 13.1%. In comparison with the sequences of A. thaliana in GenBank, the RAPD fragments in T80II changed greatly in base sequences with an average rate of one base change per 16.8 bases [46] . But different results were obtained by RAPD analysis in hinoki cypress shoot primordial irradiated by 4He 2+ and 12C 5+ heavy ion beam.…”