SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

Deng, Chuan‐Liang; Qin, Rui; Gao, Jianrong; Jia, Yong-qian; Yu, R.; Gao, Wu‐Jun; Longdou, Lu

doi:10.4238/2013.february.7.3

Cited by 7 publications

(3 citation statements)

References 13 publications

(10 reference statements)

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“…Solanum villosum and Solanum sisymbriifolium are two important wild relatives of eggplant. Sequence-related amplified polymorphism (SRAP) technology has been recognized as a new and useful molecular marker system for mapping and gene tagging in many crops plants [16]. Sequence characterized amplified region (SCAR) markers are more reproducible and easier to manipulate in marker-assisted selection (MAS) programs than other markers.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial wilt resistant gene searching in Eggplant (Solanum melongena) and its two wild relatives

Zohura¹,

Hoque²

2019

IJEAB

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Eggplant is an important vegetable in all over the world. Bacterial wilt caused by Ralstonia solanacearum is a major disease of eggplant. Near about 32% crop loss occurred due to this disease. The wild relatives of eggplant viz. Solanum villosum and Solanum sisymbriifolium showed resistant against bacterial wilt disease. Hence, attempted was made to search the resistant gene of bacterial wilt in three Solanum spp. Some total of 16 different bacterial wilt resistant molecular markers RAPD (OPB-17, OPG-05, OPH-07) UBC (176, 205, 317) SSR (STM0007, emh01J23, emb01N07) SCAR (me1, me2, me4, me5) and SRAP (SCU176-534, SCU176-1190F1R1, SCU176-1190F2R2) were screened to identified the resistant DNA fragment. Only one primer UBC#176 showed amplification in target DNA. The primer UBC#176 gave DNA amplification in target position at 350 bp in the species of Solanum villosum. The obtained DNA fragment showed maximum 79% homology with Solanum lycopersicum cultivar I-3 chromosome 8 which has GeneBank Accession No. CP023764.1. Alternative approach was made to identify resistant gene of bacterial wilt disease. Total RNA was extracted and cDNA was synthesized. The synthesized cDNA was used as template to find out the resistant gene. But none of the gene specific primer was amplified using cDNA as template. Further study was needed to find out the bacterial wilt resistant DNA fragment in wild type.

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Section: Introductionmentioning

confidence: 99%

“…SCAR can be considered to be an ideal marker for plant breeding programs. It was screened that two SCAR markers linked to Fusarium wilt resistance gene in eggplant [16]. Hence, three Solanum spp.…”

Section: Introductionmentioning

confidence: 99%

Bacterial wilt resistant gene searching in Eggplant (Solanum melongena) and its two wild relatives

Zohura¹,

Hoque²

2019

IJEAB

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“…Sequence-related amplified polymorphism (SRAP) technology has been recognized as a new and useful molecular marker system for mapping and gene tagging in many crops plants (Valdez-Ojeda et al, 2008;Cao et al, 2011;Niu et al, 2011;Guo et al, 2012;Deng et al, 2013). They are also useful in positional cloning of genes and in elucidating the genetic mode of complex traits that do not display Mendelian segregation (Levi, 2002).…”

Section: Introductionmentioning

confidence: 99%

Identification and utility of sequence related amplified polymorphism (SRAP) markers linked to bacterial wilt resistance genes in potato

Ye¹,

Li²,

Zhang³

et al. 2014

Afr. J. Biotechnol.

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Bacterial wilt caused by Ralstonia solanacearum is one of the most economically important diseases affecting potato (Solanum tuberosum). It is necessary to develop more molecular markers for potential use in potato genetic research. A highly resistant primitive cultivated species Solanum phureja was employed to generate a F1 mapping population to perform the bulked segregant analysis (BSA) for screening and identifying of sequence related amplified polymorphism (SRAP) markers linked to the potato resistance to bacterial wilt. A linkage map containing 23 DNA markers distributed on three linkage groups, and covering a genetic distance of 111 cm with an average distance of 5.8 cm between two markers was developed. Two SRAP markers, Me2em5 linked in repulsion phase and Me2em2 in coupling phase, flanked the resistance genes at genetic distances of 3.5 and 3.7 cm distance, respectively. These markers and two others were used for early seedling selection in a BC1 population. The results show that this marker system could be used in marker assisted selection (MAS) breeding program.

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Oxidative damage and cell-programmed death induced in Zea mays L. by allelochemical stress

et al. 2015

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The allelochemical stress on Zea mays was analyzed by using walnut husk washing waters (WHWW), a by-product of Juglans regia post-harvest process, which possesses strong allelopathic potential and phytotoxic effects. Oxidative damage and cell-programmed death were induced by WHWW in roots of maize seedlings. Treatment induced ROS burst, with excess of H2O2 content. Enzymatic activities of catalase were strongly increased during the first hours of exposure. The excess in malonildialdehyde following exposure to WHWW confirmed that oxidative stress severely damaged maize roots. Membrane alteration caused a decrease in NADPH oxidase activity along with DNA damage as confirmed by DNA laddering. The DNA instability was also assessed through sequence-related amplified polymorphism assay, thus suggesting the danger of walnut processing by-product and focusing the attention on the necessity of an efficient treatment of WHWW.

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SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

Cited by 7 publications

References 13 publications

Bacterial wilt resistant gene searching in Eggplant (Solanum melongena) and its two wild relatives

Bacterial wilt resistant gene searching in Eggplant (Solanum melongena) and its two wild relatives

Identification and utility of sequence related amplified polymorphism (SRAP) markers linked to bacterial wilt resistance genes in potato

Oxidative damage and cell-programmed death induced in Zea mays L. by allelochemical stress

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