Bioeffects of Low Energy Ion Beam Implantation: DNA Damage, Mutation and Gene Transter

Abstract

Low-energy ion beam implantation (10 ∼ 200 keV) has been proved to have a wide range of biological effects and is broadly used in the breeding of crops and micro-organisms.To understand its mechanisms better and facilitate its applications, the developments in the bioeffects of low energy ion beam implantation in the past twenty years are summarized in this paper.

Towards the Possibility of Additive Manufacturing of XNA-Based Devices Using Molecular Engineering Principles

“…In particular, it is possible to perform simultaneous optical and electron-beam exposure in a CLEM (correlated light and electron microscopy) chamber [106,107]. The procedure can be limited to the electron-beam and ionic processing of the sample according to the standard protocol using ESEM (on the living samples [108,109]) or LVEM [110,111] (which is also suitable for the samples that maintain biochemical functions during exposure) or FIB-SEM [112][113][114][115]. Polymer melting can be carried out either in a microdischarge (including corona discharge or a stray / traveling over the dielectric surface discharge) directly in the SEM / ESEM chamber [116][117][118] or, if microdischarge technique is not available, on a heated microscope table [119].…”

The Mutation Breeding and Mutagenic Effect of Air Plasma onPenicillium Chrysogenum

“…Several physical and chemical mutagens have been used to deal with microbial cells to obtain high-yielding strains, including ultraviolet rays, X-rays, microwaves, alkylating agents and nitrites [1] . Compared with chemical mutagens, many of which are carcinogenic to human beings [2] , physical mutagens that emit several kinds of rays as mutation sources are more widely used in industrial microbial fields, and they can be easily operated and have positive mutagenic effects [3] . Although a little progress has been achieved in the fermentation industry, the sensitivity of industrial strains to mutagens declines and the saturation to mutation occurs due to repeated use of traditional mutation methods [4] .…”