The Mutation Breeding and Mutagenic Effect of Air Plasma onPenicillium Chrysogenum

Abstract

Low temperature air plasma was used as the mutation tool for penicillin-producing strain P enicillium chrysogenum. The discharge conditions were RF power of 360 W, temperature of 40 o C in a sealed chamber, and pressure of 10 Pa to 30 Pa. The result showed that the kinetics of the survival rate followed a typical saddle-shaped curve. Based on a statistic analysis, at the treating duration of 10 min, the positive mutation rate was as high as 37.5% while the negative mutation rate was low. The colonial morphology changed obviously when the plasma treating duration reached or exceeded 45 min. After both primary and secondary screening, a mutant designated as aPc051310 with high productivity of penicillin was obtained, and a strong mutagenic effect on P. chrysogenum was observed in the process. It was proved that after five generations, the mutant aPc051310 still exhibits a high productivity. All the results prove that the plasma mutation method could be developed as a convenient and effective tool to breed high-yield strains in the fermentation industry, while expanding the plasm application at the same time.

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

“…It requires only a small amount of energy input to generate stable plasma discharge at atmospheric pressure and excite a plasma jet with high concentrations of reactive particles (excited helium atoms, hydrogen atoms, and oxygen atoms), 34,35 playing an important role in the field of microbial mutagenesis. In addition to this, the use of gases such as argon, air, oxygen, and helium has also been reported elsewhere 17,18,36 . In general, the generated reactive species in plasma discharge depend to a large extent on the composition of the working gases.…”

“…In addition to this, the use of gases such as argon, air, oxygen, and helium has also been reported elsewhere. 17,18,36 In general, the generated reactive species in plasma discharge depend to a large extent on the composition of the working gases. The addition of nitrogen or oxygen to the inert gases increases the variety of reactive chemicals, 37 ), nitric oxide (NO) and ozone (O 3 ).…”

Discharge and Optical Emission Spectrum Characteristics of a Coaxial Dielectric Barrier Discharge Plasma-Assisted Combustion Actuator

“…Plasma, which is an equal density of negative and positive particles ionized gas, belongs to the fourth state of matter and is composed of charged particles (electrons and ions), atoms, radicals, molecules in ground and excited states, and photons [1,2]. Plasma has been widely used in many fields, such as plasma-assisted combustion (PAC) [3], plasma surface modification [4], plasma sterilization [5], plasma active flow control [6], and plasma mutation breeding [7]. Among these, PAC is a remarkable solution to enlarging stable combustion boundary, improving combustion stability and combustion efficiency, and lowering nitric oxide emissions; it addresses these issues at a low energy cost, as put forward and studied by numerous relevant experts [8][9][10][11].…”