Goal. To determine the parameters of changes in the content of organic carbon in typical chernozem depending on the long-term use of different fertilizer systems for field crop rotation in the High Forest-Steppe of the Left Bank of Ukraine. Methods. Field -to determine the impact of fertilizer application systems on the humus state of typical chernozem, laboratory -to determine the content of organic carbon in the soil, calculation -to calculate the reserves of organic carbon and humus, mathematical -to assess the reliability of the data. Results. It was determined that before the establishment of a stationary field experiment, the content of organic carbon (Corg) in the arable soil layer averaged 2.89%. The coefficient of variation of theparameters of this indicator was 8%, but the scope of variation of the obtained results was 1.03 abs. %. It was established that for 28 years of application of mineral and organic fertilizers there was a slow deposition of Corg. Under the mineral fertilizer system with the introduction of single and double doses of complete fertilizer, the Corg content increased from 2.94 -2.96 to 3.09 -3.10%. Under the organic system, which provided the periodic application of manure under row crops, the Corg content increased from 2.80 to 3.09%, while the combined application of manure and mineral fertilizers caused an increase in Corg from 2.71 -2.79 to 3.09 -3.16%. Corg reserves increased from 5 for the mineral to 10 and 13 t/ha for organic and organomineral fertilizer systems, respectively. Conclusions. An increase in the Corg content in typical chernozem was established with the introduction of fertilizer systems in a row: mineral -+0.14 -0.15 abs.%, organic -+0.29, organomineral -+0.37 -0.38 abs.%. The systematic application of mineral and periodic of organic fertilizers contributes the most to carbon deposition in soils.