Challenge in understanding size and shape dependent toxicity of gold nanomaterials in human skin keratinocytes

Wang, Shuguang; Lu, Wentong; Tovmachenko, O. G.; Shanker, Uma; Yu, Hongtao; Ray, Paresh Chandra

doi:10.1016/j.cplett.2008.08.039

Cited by 322 publications

(246 citation statements)

References 29 publications

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“…Similar results were obtained for 3 nm AuNPs, 5nm, 5, 12, 20, 30, 50, and 70nm, 3, 5, 8, 12, 17, 37, 50, and 1200nm, 4 and 13nm, and 40nm 112 [53][54][55][56][57][58]. Taking into account the abovementioned data, it could be possible to expand the boundaries to both the lower level (to 3 nm) and larger particles up to 100 nm to avoid a toxic AuNP effect, if we are considering a limited dose of about 10 12 particles/ml.…”

Section: Particle Size Effectsupporting

confidence: 74%

“…Nanoparticles have high surface-to-volume ratios when compared with large particles and are usually smaller than several hundred nanometers in size, comparable to large biological molecules, of a size about 100 to 10,000 times smaller than human cells. The range of studied gold nanoparticles covers more than two orders of magnitude from the minimal size of 1.0, 1.2 or 1.4 nm (atomic cluster Au 15 , Au 35 or Au 55 ) [2] to the maximal size of 250 nm [3].…”

Section: Introductionmentioning

confidence: 99%

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On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Capek¹

2015

JNMR

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Vocabulary EndocytosisIt is an energy-using process by which cells absorb molecules (such as proteins) by engulfing them. It is used by all cells of the body because most substances important to them are large polar molecules that cannot pass through the hydrophobic plasma or cell membrane. The opposite process is exocytosis. Lactate dehydrogenase (LDH)It is of medical significance because it is found extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease. The MTT assayIt is a colorimetric assay for assessing cell viability. WSTs (water soluble tetrazolium salts)These are a series of other water soluble dyes for MTT assays, developed to give different absorption spectra of the formed formazans. WST-1 is advantageous over MTT in that it is reduced outside cells. WST assays (1) can be read directly (unlike MTT that needs a solubilization step), give a more effective signal than MTT, and decrease toxicity to cells (unlike cell-permeable MTT, and its insoluble formazan that accumulate inside cells). BiotinIt also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). Biotin has an unusual structure, with two rings fused together via one of their sides. The two rings are ureido and thiophene moieties. Biotin is a heterocyclic, S-containing monocarboxylic acid.

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Section: Particle Size Effectsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Capek¹

2015

JNMR

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“…The template was removed with a methanol acidic wash because CTAB has been shown to be toxic for cells 41 due to its capability to damage biological membranes and cause the release of intracellular enzymes. The effective removal of CTAB was confirmed with FT-IR ( Figure 1A and B).…”

Section: Characterization Of Msnpsmentioning

confidence: 99%

Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos

Sharif¹,

Porta²,

Meijer³

et al. 2012

IJN

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Silica nanoparticles can be efficiently employed as carriers for therapeutic drugs in vitro. Here, we use zebrafish embryos as a model organism to see whether mesoporous silica nanoparticles (MSNPs) can be incorporated to deliver compounds in vivo. We injected 35-40 nL (10 mg/mL) of custom-synthesized, fluorescently-tagged 200 nm MSNPs into the left flank, behind the yolk sac extension, of 2-day-old zebrafish embryos. We tracked the distribution and translocation of the MSNPs using confocal laser scanning microscopy. Some of the particles remained localized at the injection site, whereas others entered the bloodstream and were carried around the body. Embryo development and survival were not significantly affected by MSNP injection. Acridine orange staining revealed that MSNP injections did not induce significant cell death. We also studied cellular immune responses by means of lysC::DsRED2 transgenic embryos. MSNP-injected embryos showed infiltration of the injection site with neutrophils, similar to controls injected with buffer only. In the same embryos, counterstaining with L-plastin antibody for leukocytes revealed the same amount of cellular infiltration of the injection site in embryos injected with MSNPs or with buffer only. Next, we used MSNPs to deliver two recombinant cytokines (macrophage colony-stimulating factor and receptor for necrosis factor ligand) to zebrafish embryos. These proteins are known to activate cells involved in bone remodeling, and this can be detected with the marker tartrate-resistant acid phosphatase. Coinjection of these proteins loaded onto MSNPs produced a significant increase in the number of tartrate-resistant acid phosphatase-positive cells after 2-3 days of injection. Our results show that MSNPs can be used to deliver bioactive compounds into zebrafish larvae without producing higher mortality or gross evidence of teratogenicity.

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“…These fine particles have unique properties that make them biologically active with affinity to accumulate in the vital organs mainly kidney liver and spleen [3][4][5] . Previous research work demonstrated the potential toxic effect of GNPs due to their size, surface area, shape, and charge where smaller particles are more toxic than the larger ones [6][7][8] . GNPs demonstrated oxidative stress and macromolecules interaction that could result in histocytotoxicity [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Protective role of propolis against histological alterations in renal tissue induced by gold nanoparticles

Al‐Μansour¹,

Alferah²,

Jarrar³

2016

ScienceAsia

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ABSTRACT:Male albino Wistar rats were exposed to 10 nm gold nanoparticles at a dose of 2000 µg/kg together with or without propolis (50 mg/kg) for 15 consecutive days. Fresh renal biopsies from of all investigated rats were cut rapidly, fixed in neutral buffered formalin, subjected to histological processing, and examined for microanatomical alterations. Propolis gave full protection against glomerular congestion and renal tubule hyaline casts. It demonstrated partial amelioration against glomerular capillary dilatation, tubular cloudy swelling, necrosis, and degeneration together with interstitial blood capillaries dilatation and haemorrhage induced by nanoparticle toxicity. On the other hand, propolis showed no protective effect against renal cells pyknosis, karyolysis, apoptosis and renal hydropic degeneration together with collecting tubules atrophy and degeneration induced by the nanoparticles. Thus propolis can augment the antioxidant defence against the severity of some alterations in the renal tissues. This ameliorative role might be related to the antioxidants content of propolis that protect the renal tissues from free radicals and oxidative stress.

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Challenge in understanding size and shape dependent toxicity of gold nanomaterials in human skin keratinocytes

Cited by 322 publications

References 29 publications

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos

Protective role of propolis against histological alterations in renal tissue induced by gold nanoparticles

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