CFTR chloride channel activity modulates the mitochondrial morphology in cultured epithelial cells

García, Rocío María Contestín; Falduti, Camila; Clauzure, Mariángeles; Jara, Raquel Muñoz; Massip-Copiz, María Macarena; Aguilar, María de los Ángeles; Santa‐Coloma, Tomás A.; Valdivieso, Ángel G.

doi:10.1016/j.biocel.2021.105976

Cited by 8 publications

(5 citation statements)

References 58 publications

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“…Drp1 and Mfn2 are known to be crucial mediators of mitochondrial function. 32,33 Drp1 is often upregulated during mitochondrial fission, whereas MFN1 is downregulated during that process. 34,35 Yang et al 36 found that the squamosamide derivative FLZ could inhibit mitochondrial fission via downregulation of Drp1; Liu et al 37 reported that HMGB1 could inhibit mitochondrial fission in lung cancer cells via mediation of Drp1 and MFN1.…”

Section: Discussionmentioning

confidence: 99%

Puerarin alleviates LPS-induced H9C2 cell injury by inducing mitochondrial autophagy

Chang

Luo

et al. 2022

Journal of Cardiovascular Pharmacology

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Sepsis leads to the damage of multiple organs, and thereby adversely affects the cardiovascular system. At present, no effective method has been found to treat myocardial injury caused by sepsis. Although Puerarin was reported to attenuate lipopolysaccharide (LPS)-induced mitochondrial injury in H9C2 cells, the effects of Puerarin in sepsis-induced myocardial injury remain unclear. In this study, H9C2 cells were stimulated with LPS, CCK-8 assays were performed to assess cell viability, and flow cytometry and TUNEL staining were used to assess cell apoptosis. Levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and enzyme activity were investigated using commercial kits. Reactive oxygen species (ROS) levels in H9C2 cells were detected by flow cytometry. Autophagosomes in the mitochondria of H9C2 cells were observed by transmission electron microscope, and protein expression was assessed by western blotting. Furthermore, in vivo experiments were applied to test the function of Puerarin in sepsis. We found that Puerarin significantly reversed LPS-induced decreases in H9C2 cell viability by inhibiting apoptosis. The ROS levels in H9C2 cells were significantly upregulated by LPS, but that effect was markedly reduced by Puerarin. In addition, Puerarin attenuated LPS-induced mitochondrial injury in H9C2 cells by regulating dynamin-related protein 1 (Drp1) and mitofusin 1 (MFN1). LPS decreased enzyme activity and reduced the levels of ADP, ALP, and AMP in mitochondria; however, those effects were reversed by Puerarin. Puerarin and Torin1 reversed LPSinduced inhibition of autophagy in the mitochondria of H9C2 cells via mediation of p62, LC3B, Pink1, and Parkin. Puerarin notably inhibited the progression of sepsis in vivo. Puerarin inhibited LPSinduced H9C2 cell injury by inducing mitochondrial autophagy, which acts as a mechanism for preventing myocardial injury caused by sepsis.

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Section: Discussionmentioning

confidence: 99%

Puerarin alleviates LPS-induced H9C2 cell injury by inducing mitochondrial autophagy

Chang

Luo

et al. 2022

Journal of Cardiovascular Pharmacology

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“…FDA approved treatments for COPD. NCT01691885 RELOVAIR NCT00633217 Advair HFA NCT0246556, NCT02497001 PT010 inhaled corticosteroid/LAMA/LABA NCT0319781, NCT01911364 CHF 5993 NCT02696564 Losartan Angiotensin II receptor type 1 antagonist Angiotensin II upregulates Mfn2, therefore inhibition likely increases mt-fission [ 293 ] No significant improvement NCT04072887 Icenticaftor activates cystic fibrosis transmembrane conductance regulator Likely decreases mt-fission and increases mt-fusion [ 294 ] Phase II trial with 974 subjects; Did not improve trough FEV at 12 weeks (primary outcome) but positive findings at 24 weeks NCT00000621 Retinoic Acid Retinoic acid receptor agonist Likely increases fission by increasing Drp1 levels [ 295 ] No significant improvement …”

Section: Therapeutic Targeting Of Mitochondrial Network Dynamicsmentioning

confidence: 99%

Mitochondrial network dynamics in pulmonary disease: Bridging the gap between inflammation, oxidative stress, and bioenergetics

Pokharel,

Garcia-Flores,

Marciano

et al. 2024

Redox Biology

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“…CFTR is known for its proapoptotic effects and growing evidence is linking ANO6 to be a regulator of cell death [89,90]. Similarly, VDAC1 (Voltagedependent anion-selective channel 1) is associated with mitochondrial dynamics including regulating apoptosis and autophagy [91]. It has been speculated that VDAC1 could be regulated and closely related to CFTR function as CFTR inhibitors have been reported to decrease VDAC1 expression [91].…”

Section: The Cftr Surfaceome Includes a Diverse Set Of Membrane Trans...mentioning

confidence: 99%

“…Similarly, VDAC1 (Voltagedependent anion-selective channel 1) is associated with mitochondrial dynamics including regulating apoptosis and autophagy [91]. It has been speculated that VDAC1 could be regulated and closely related to CFTR function as CFTR inhibitors have been reported to decrease VDAC1 expression [91]. Lastly, six members of the ABC transporter superfamily were found in the surfaceome (ABCB10, ABCB7, ABCC1, ABCC11, ABCD3, ABCE1) (Figure 5).…”

Section: The Cftr Surfaceome Includes a Diverse Set Of Membrane Trans...mentioning

confidence: 99%

A Proteomic Survey of the Cystic Fibrosis Transmembrane Conductance Regulator Surfaceome

Iazzi

Sadeghi

Gupta

2023

IJMS

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The aim of this review article is to collate recent contributions of proteomic studies to cystic fibrosis transmembrane conductance regulator (CFTR) biology. We summarize advances from these studies and create an accessible resource for future CFTR proteomic efforts. We focus our attention on the CFTR interaction network at the cell surface, thus generating a CFTR ‘surfaceome’. We review the main findings about CFTR interactions and highlight several functional categories amongst these that could lead to the discovery of potential biomarkers and drug targets for CF.

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CFTR chloride channel activity modulates the mitochondrial morphology in cultured epithelial cells

Cited by 8 publications

References 58 publications

Puerarin alleviates LPS-induced H9C2 cell injury by inducing mitochondrial autophagy

Puerarin alleviates LPS-induced H9C2 cell injury by inducing mitochondrial autophagy

Mitochondrial network dynamics in pulmonary disease: Bridging the gap between inflammation, oxidative stress, and bioenergetics

A Proteomic Survey of the Cystic Fibrosis Transmembrane Conductance Regulator Surfaceome

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