Cerium oxide nanoparticle-containing poly (ε-caprolactone)/gelatin electrospun film as a potential wound dressing material: In vitro and in vivo evaluation

Naseri-Nosar, Mahdi; Farzamfar, Saeed; Sahrapeyma, Hamed; Ghorbani, Sadegh; Bastami, Farshid; Vaez, Ahmad; Salehi, Majid

doi:10.1016/j.msec.2017.08.013

Cited by 134 publications

(64 citation statements)

References 44 publications

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“…Comparing the absorbance values of the control, PCL, and PCL/CNF groups showed that PCL and PCL/CNF scaffolds had no significant toxicity toward seeded cells. This is in accordance with previous studies which have reported proper cytocompatibility of CNF and PCL (Mirzaei et al 2016;Naseri-Nosar et al 2017).…”

Section: Cell Viability Studiessupporting

confidence: 94%

A novel polycaprolactone/carbon nanofiber composite as a conductive neural guidance channel: an in vitro and in vivo study

et al. 2019

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The current study aimed to investigate the potential of carbon nanofibers to promote peripheral nerve regeneration. The carbon nanofiber-imbedded scaffolds were produced from polycaprolactone and carbon nanofibers using thermally induced phase separation method. Electrospinning technique was utilized to fabricate polycaprolactone/collagen nanofibrous sheets. The incorporation of carbon nanofibers into polycaprolactone's matrix significantly reduced its electrical resistance from 4.3 × 10 9 ± 0.34 × 10 9 Ω to 8.7 × 10 4 ± 1.2 × 10 4 Ω. Further in vitro studies showed that polycaprolactone/carbon nanofiber scaffolds had the porosity of 82.9 ± 3.7% and degradation rate of 1.84 ± 0.37% after 30 days and 3.58 ± 0.39% after 60 days. The fabricated scaffolds were favorable for PC-12 cells attachment and proliferation. Neural guidance channels were produced from the polycaprolactone/carbon nanofiber composites using water jet cutter machine then incorporated with PCL/ collagen nanofibrous sheets. The composites were implanted into severed rat sciatic nerve. After 12 weeks, the results of histopathological examinations and functional analysis proved that conductive conduit out-performed the non-conductive type and induced no toxicity or immunogenic reactions, suggesting its potential applicability to treat peripheral nerve damage in the clinic.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Cell Viability Studiessupporting

confidence: 94%

A novel polycaprolactone/carbon nanofiber composite as a conductive neural guidance channel: an in vitro and in vivo study

et al. 2019

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“…In addition, nanofibers as dressing improved wound healing physically. There are several publications reporting the use of nanofibers for wound treatment in mice [11,57,58] and rats [59][60][61][62]; however, to the best of our knowledge this is the first work on in vivo wound healing of a in situ gel forming βG-nanofibers, with βG isolated from beakers yeast.…”

Section: In Vivo Wound Healing In Diabetic Micementioning

confidence: 99%

Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice

Grip

Engstad

Skjæveland

et al. 2018

European Journal of Pharmaceutical Sciences

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The increased prevalence of chronic wounds requires novel treatment options. The aim of this study was to develop a beta-glucan (βG)-loaded nanofiber wound dressing. Nanofibers were prepared using the needle-free Nanospider™ technology, an electrospinning method which enables the production of nanofibers at an industrial scale. The βG was selected as active ingredient based on its confirmed wound healing potential in both animals and humans. Hydroxypropyl methylcellulose (HPMC) and polyethylene oxide (PEO) were included as copolymers. Rheological profiles of spinning solutions containing HPMC, PEO, βG, ethanol and water, were optimized. The nanofiber formation was confirmed by Field Emission Scanning Electron Microscopy (FE-SEM), and both nanofibers with (βG-nanofibers) or without βG (NoβG-nanofibers) were evaluated by their swelling index and FT-IR spectroscopy. The formulations, active ingredient and excipients were tested for their possible in vitro toxicity in keratinocytes. Finally, the wound healing potential of the nanofibers was tested in externally induced excisional wounds in male diabetic db/db mice. Three different doses of βG-nanofibers and the βG-free, NoβG-nanofibers, were evaluated for their in vivo wound healing efficacy. All nanofiber-treatments provided improved wound healing as compared to the negative control (water). All βG-nanofiber treated groups exhibited significantly improved wound healing as compared to the NoβG-nanofiber treated group, indicating the potential of βG-nanofibers as wound dressing.

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“…Bio‐scaffolds are often fabricated to increase the interactions of nanoscale surfaces with cell receptors, which are directly involved in promoting cell attachment, migration, growth, and differentiation. Electrospun nanofiber matrices are the best candidates for mimicking the natural ECM of specific tissues because of the fact that they enjoy ultrafine continuous fibers, high surface‐to‐volume ratio, high porosity, and variable pore‐size distribution …”

Section: Introductionmentioning

confidence: 99%

Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin

Ghorbani

Eyni

Khosrowpour

et al. 2019

Polymers for Advanced Techs

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Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multiwalled carbon nanotubes (MWCNTs) were incorporated into poly(L-lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse-transcription polymerase chain reaction (RT-PCR), and qRT-PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C-kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories. KEYWORDS antioxidants, in vitro spermatogenesis, naringenin, PLLA/MWCNTs scaffolds, spermatogonial stem cells (SSCs) Abbreviations: bp, base pairs; C-Kit, proto-oncogene c-Kit or tyrosine-protein kinase Kit; DMEM, Dulbecco's modified eagle medium; DMF, N, N-dimethyl formamide; ECM, natural extra cellular matrix; FTIR, Fourier transform-infrared spectroscopy; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICSI, intra-cytoplasmic sperm injection; Id4, inhibitor of differentiation 4; IVM, in vitro oocyte maturation; m-TESE, testicular sperm extraction; PLLA/MWCNTs, poly (L-lactic acid)/multi-walled carbon nanotube; PLZF, promyelocytic leukemia zinc finger; ROS, reactive oxygen species; ROSI, round spermatid injection; RT-PCR, reverse-transcription polymerase chain reaction; SEM, scanning electron microscopy; SSCs, spermatogonial stem cells; SYCP3, synaptonemal complex protein 3

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Cerium oxide nanoparticle-containing poly (ε-caprolactone)/gelatin electrospun film as a potential wound dressing material: In vitro and in vivo evaluation

Cited by 134 publications

References 44 publications

A novel polycaprolactone/carbon nanofiber composite as a conductive neural guidance channel: an in vitro and in vivo study

A novel polycaprolactone/carbon nanofiber composite as a conductive neural guidance channel: an in vitro and in vivo study

Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice

Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin

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