Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice

Grip, Jostein; Engstad, Rolf Einar; Skjæveland, Ingrid; Škalko‐Basnet, Nataša; Isaksson, Johan; Basnet, Purusotam; Holsæter, Ann Mari

doi:10.1016/j.ejps.2018.05.031

Cited by 60 publications

(37 citation statements)

References 70 publications

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“…There are no previous reports of the electrospinning of this mixture of polymers; however, similar diameters have been reported in a range of 80-120 nm for fibers made from glucans. 31 In sum, pure solutions of PCL (in chloroform) and the polysaccharide pullulan (in water) presented excellent electrospinnability, and homogeneous fibers with submicron diameters were obtained, with low bead formation, in agreement with previous evidence. In contrast, chloroform solutions of mixtures of PCL with the polysaccharides PMS and β-glucan allowed for obtaining electrospun fibers; however, the electrospinning process was unstable, and the materials obtained presented a more irregular morphology and exhibited the presence of beads.…”

Section: Preparation and Morphology Of Nanofibrous Membranessupporting

confidence: 88%

Ultrathin single and multiple layer electrospun fibrous membranes of polycaprolactone and polysaccharides

Rodríguez-Sánchez

Vergara-Villa

Clavijo-Grimaldo

et al. 2020

Journal of Bioactive and Compatible Polymers

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Electrospinning was used to produce fibrous membranes, in single and multiple layers, from poly(ε-caprolactone), pullulan, and from mixtures of poly(ε-caprolactone) with potato modified starch and β-glucan. It was possible to obtain single-layer membranes from solutions of pullulan in water, poly(ε-caprolactone) in chloroform, and from mixtures of poly(ε-caprolactone)/β-glucan and poly(ε-caprolactone)/potato modified starch in chloroform. Scanning electron microscopy images showed the formation of ultrathin homogeneous fibers from electrospun poly(ε-caprolactone) and pullulan, whereas the fibers obtained from mixtures of poly(ε-caprolactone)/ β -glucan and poly(ε-caprolactone)/potato modified starch had different sizes and morphologies, as well as irregular microstructures, characterized by the presence of beads. Contact angle analyses showed that pullulan membranes were extremely hydrophilic, while poly(ε-caprolactone) membranes were predominantly hydrophobic. Subsequently, poly(ε-caprolactone)-pullulan-poly(ε-caprolactone) multilayer membranes, with intermediate wettability, were prepared by successive electrospinning steps. Infrared spectroscopy and calorimetric analyses showed the presence of both polymers and the absence of changes in their structure and stability due to electrospinning, indicating adequate compatibility between the two polymers. We foresee that the polyester-polysaccharide multilayer membrane might be used as a biodegradable vehicle for active agents with different hydrophobicity, with applications as food packaging and biocompatible scaffold materials.

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Section: Preparation and Morphology Of Nanofibrous Membranessupporting

confidence: 88%

Ultrathin single and multiple layer electrospun fibrous membranes of polycaprolactone and polysaccharides

Rodríguez-Sánchez

Vergara-Villa

Clavijo-Grimaldo

et al. 2020

Journal of Bioactive and Compatible Polymers

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“…An efficient wound dressing must be capable of removing the exudates at the wound site. In this regard, the absorption capacity of uncross-linked ZNFs (F2), CA cross-linked ZNFs (F9–F12) and BIS-loaded 7% w/w C-ZNFs (F13–F15) scaffolds were studied using two different approaches: the moisture uptake52 and a modified swelling index method 53. For F2, the moisture uptake and swelling index values were 329% and 4.99, respectively, which promote the absorption of large amounts of wound exudates as a result of the spinning process (Figure 2D and E).…”

Section: Resultsmentioning

confidence: 99%

<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

El-Lakany¹,

Abd-Elhamid²,

Kamoun³

et al. 2019

IJN

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ObjectivesNovel α-bisabolol (BIS)-loaded citric acid cross-linked zein nanofibrous scaffolds (C-ZNFs) were proposed to serve as safe platforms for promoting wound repair in rats.MethodsZNFs were synthesized using electrospinning technique, then NFs, with adequate water resistance, were produced using citric acid as a safe cross-linker.ResultsCompared to the uncross-linked ZNFs, cross-linking with 7% w/w citric acid decreased swelling index by 3 folds, while the tensile strength and the contact angle were enhanced to 2.5 and 3.8 folds, respectively. SEM images showed beads-free homogeneous NFs with a fully inter-connected 3D-network, where the average diameter of optimized C-ZNFs was 181.7±50 nm. After 24 h, C-ZNFs exhibited a decreased BIS release rate (45.6%), compared to uncross-linked mats (84.9%). By increasing BIS concentration, the cell adhesion (WI38 fibroblasts) was improved which can be attributed mainly to BIS activation of transforming growth factor-beta (TGF-β1). The MTT-OD obtained values indicated that all tested zein scaffolds significantly enhanced the viability of WI38 fibroblasts, compared to the control after 48h of incubation which can be referred to the proliferative potential of zein by provoking cell spreading process. The scratch wound assay demonstrated that BIS-loaded ZNF scaffolds showed accelerated migration and proliferation of fibroblasts expressed by significantly higher wound closure rates compared to the control sample. BIS-loaded-C-ZNFs prominently accelerated tissue regeneration for wound closure demonstrated by entirely grown epithelium with normal keratinization and rapid wound contraction, compared to the control. Immunohistochemical results confirmed the superiority of BIS-loaded-C-ZNFs, where the observed reduced NF-κB and the elevated cytokeratin expressions confirmed the anti-inflammatory and proliferative effects of the scaffolds, respectively.ConclusionIn-vitro, optimized C-ZNFs offered a satisfactory cytocompatibility, adhesion and healing which were consistent with the in-vivo results. BIS-loaded-C-ZNFs could be regarded as a promising and effective biomaterial for tissue regeneration and for accelerating the wound healing process.

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“…15 By creating similar environments as those of body tissues, 3D nano scaffolds offer a new perspective in cell culture, drug delivery, and tissue repair. 16,18 Poly caprolactone (PCL), being a biocompatible, hydrophobic, and non-toxic polymer, is widely used as a drug carrier to prepare implantation devices and biomedical grafts in the tissue engineering field. 19,20 Many oxygen releasing agents have been tested for their potential in improving diabetic wounds but oxygen releasing biomaterials, especially peroxide based biopolymers, are of special interest for their tunable properties in terms of controlled oxygen generation at the wound site.…”

Section: Introductionmentioning

confidence: 99%

<p>Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model</p>

Zehra

Zubairi

Hasan

et al. 2020

IJN

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Introduction: Diabetic wounds are challenging to treat due to a wide range of pathophysiological changes. Hypoxia is one of the predominant contributing factors of poor vascularization and chronicity in diabetic wounds. This study was designed to develop polycaprolactone (PCL)-based oxygen-releasing electrospun wound dressings and evaluate their efficacy for improved full thickness wound healing in diabetic rats. Methods: PCL-based oxygen releasing wound dressings were made using electrospinning technology. The developed dressings were characterized in terms of physical as well as biological properties both in vitro and in vivo. E-spun nanofibrous dressings were physically characterized with scanning electron microscopy, Fourier-transform infrared spectroscopy, and Energy-dispersive X-ray spectroscopy. To study the likely impact of the fabricated wound dressings in hypoxic conditions, HIF-1α expression analysis was carried out both at gene and protein levels. Wound dressings were further evaluated for their healing potential for extensive wounds in diabetic rat models. Results: The experimental results showed that the developed dressings were capable of continuously generating oxygen for up to 10 days. Cell studies further confirmed pronounced expression of HIF-1α at gene and protein levels in cells seeded on PCL-sodium percarbonate (SPC) and PCL scaffolds compared with the cells cultured on a tissue culture plate. Chorioallantoic membrane assay revealed the supportive role of oxygen releasing dressings on angiogenesis compared to the control group. Histological assessment of the regenerated skin tissues proved that full thickness wounds covered with SPC loaded PCL dressings had a comparatively better vascularized and compact extracellular matrix with completely covered thick epithelium. Discussion: The developed oxygen generating polymeric nanofibrous wound dressings could potentially be used as an envisioned approach for the efficient recovery of chronic diabetic wounds.

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Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice

Cited by 60 publications

References 70 publications

Ultrathin single and multiple layer electrospun fibrous membranes of polycaprolactone and polysaccharides

Ultrathin single and multiple layer electrospun fibrous membranes of polycaprolactone and polysaccharides

<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

<p>Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model</p>

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