Cerium and gallium containing mesoporous bioactive glass nanoparticles for bone regeneration: Bioactivity, biocompatibility and antibacterial activity

Kurtuldu, Fatih; Mutlu, Nurshen; Michálek, Martin; Zheng, Kai; Masař, Milan; Liverani, Liliana; Chen, Si; Galusek, Dušan; Boccaccını, Aldo R.

doi:10.1016/j.msec.2021.112050

Cited by 82 publications

(35 citation statements)

References 53 publications

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“…Cerium doping resulted in a limited incorporation of both CaO and CeO 2 in the NPs, as shown from the XRF analysis. This was expected, as numerous studies [ 1 , 49 ] have shown that in these systems calcium amounts are usually lower due to the co-doping of other ions. However, the amounts of both calcium and cerium were higher compared to those reported by Kurtuldu et al [ 1 ] in the same system, and this explains the high bioactivity of the synthesized NPs, even those with the highest amount of cerium incorporation, that developed apatite after 1 day of immersion in SBF (see below).…”

Section: Resultsmentioning

confidence: 61%

“…This was expected, as numerous studies [ 1 , 49 ] have shown that in these systems calcium amounts are usually lower due to the co-doping of other ions. However, the amounts of both calcium and cerium were higher compared to those reported by Kurtuldu et al [ 1 ] in the same system, and this explains the high bioactivity of the synthesized NPs, even those with the highest amount of cerium incorporation, that developed apatite after 1 day of immersion in SBF (see below). In the present study, a second, neutral medium to dissolve the nitrate salts and TEOS was used to minimize the formation of Ca(OH) 2 or calcium and cerium oxides as byproducts.…”

Section: Resultsmentioning

confidence: 61%

“…Mesoporous materials have attracted considerable interest in the field of medicine. They present distinct advantages such as large surface area and tunable pore structure that render them ideal carriers for drug delivery, especially in combination with biologically active ions (e.g., Ca, Ce, Mg, Cu ions) [ 1 , 2 ]. In recent years, ion doping has appeared as a common strategy to improve the properties of mesoporous nanostructured biomaterials [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

et al. 2021

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Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m2/g for undoped silica to 495 m2/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells.

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

et al. 2021

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“…Likewise, the presence of several lanthanide therapeutic elements investigated to be added to MBGs, such as Ce [ 76 ], Sm [ 77 ], Eu [ 78 ], or Tb [ 79 ], must be highlighted. As is observed, many other elements are under investigation, in several cases more than one decade ago, including Ti, V, Mn, Fe, Co, Cu, Zn, Ga, and Sr [ 61 , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] ]. Moreover, studies about new elements added to MBGs are frequently published, for example, the recently investigated antibacterial and antioxidant properties of Te [ 88 ].…”

Section: Expanding Mesoporous Glass Properties By Adding Inorganic Ionsmentioning

confidence: 99%

Mesoporous bioactive glasses for regenerative medicine

Vallet‐Regí

Salinas

2021

Materials Today Bio

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Stem cells are the central element of regenerative medicine (RM). However, in many clinical applications, the use of scaffolds fabricated with biomaterials is required. In this sense, mesoporous bioactive glasses (MBGs) are going to play an important role in bone regeneration because of their striking textural properties, quick bioactive response, and biocompatibility. As other bioactive glasses, MBGs are mainly formed by silicon, calcium, and phosphorus oxides whose ions play an important role in cell proliferation as well as in homeostasis and bone remodeling process. A common improvement of bioactive glasses for RM is by adding small amounts of oxides of elements that confer them additional biological capacities, including osteogenic, angiogenic, antibacterial, anti-inflammatory, hemostatic, or anticancer properties. Moreover, MBGs are versatile in terms of the different ways in which they can be processed, such as scaffolds, fibers, coatings, or nanoparticles. MBGs are unique because their textural properties are so high that they still exhibit outstanding bioactive responses even after adding extra inorganic ions or being processed as scaffolds or nanoparticles. Moreover, they can be further improved by loading with biomolecules, drugs, and stem cells. This article reviews the state of the art and future perspectives of MBGs in the field of RM of hard tissues.

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“…The Ce-containing MBGNPs exhibited anti-inflammatory effects and pro-osteogenic activity [ 20 ]. The synthesis of cerium-containing MBGNPs in the system of SiO 2 –CaO with a one-step approach by microemulsion assisted sol-gel method was reported in our recent publication [ 35 ]. The study confirmed the bioactive behavior of the prepared MBGNPS doped with 1 mol% Ce 2 O 3 .…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications

Kurtuldu

Kaňková

Beltrán

et al. 2021

Materials Today Bio

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Mesoporous bioactive glass nanoparticles (MBGNPs) are attracting significant attention as suitable materials for multifunctional biomedical applications. In this study, cerium was incorporated into MBGNPs using two different approaches. In the first approach, cerium was added to the glass system directly during the synthesis, while in the second approach, cerium was added to the as-synthesized MBGNPs via the template ion-exchange method. The influence of the method of synthesis on the physicochemical properties of nanoparticles was examined by SEM, TEM, XRD, FTIR, and N 2 adsorption-desorption analyses. The MBGNPs exhibited spheroidal morphology and disordered mesoporous structure. XRD analysis confirmed the amorphous nature of the nanoparticles. The chemical composition was determined by the acid digestion method using ICP-OES. The influence of the synthesis method on the specific surface area, mesoporosity, and solubility of synthesized nanoparticles in Tris/HCl (pH 7.4) and acetate (pH 4.5) buffer has also been studied. The obtained Ce containing MBGNPs were non-cytotoxic toward preosteoblast MC3T3-E1 cells in contact with nanoparticles in a concentration of up to 100 μg/mL. The anti-inflammatory effect of Ce containing MBGNPs was tested with lipopolysaccharides (LPS)-induced proinflammatory RAW 264.7 macrophage cells. Ce containing MBGNPs decreased the release of nitric oxide, indicating the anti-inflammatory response of macrophage cells. Ce containing MBGNPs also showed antibacterial activity against S. aureus and E. coli . The mentioned features of the obtained MBGNPs make them useful in a variety of biomedical applications, considering their biocompatibility, anti-inflammatory response, and enhanced antibacterial effect.

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Cerium and gallium containing mesoporous bioactive glass nanoparticles for bone regeneration: Bioactivity, biocompatibility and antibacterial activity

Cited by 82 publications

References 53 publications

Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

Mesoporous bioactive glasses for regenerative medicine

Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications

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