Cephamycin Production by Streptomyces Clavuligerus

Demain, Arnold L.; Piret, Jacqueline

doi:10.1007/978-1-4684-5922-7_12

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Cited by 5 publications

(1 citation statement)

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“…Two monomycin antibiotics are selectively produced by Streptomyces jamaicensis when the phosphate is 0.1 mM or 0.4 mM (Hall and Hassall, 1970). Phosphate regulation has also been discussed by Weinberg (1974), Aharonowitz and Demain (1977), Martin and Demain (1980), Iwai and Omura (1982) and Demain and Piret (1991).…”

Section: Mineralsmentioning

confidence: 93%

Media for Industrial Fermentations

Stanbury

1995

Principles of Fermentation Technology

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Section: Mineralsmentioning

confidence: 93%

Media for Industrial Fermentations

Stanbury

1995

Principles of Fermentation Technology

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Lignin Peroxidase and Protease Production by Streptomyces viridosporus T7A in the Presence of Calcium Carbonate

Macedo

Gottschalk

Bon

1999

Twentieth Symposium on Biotechnology for Fuels and Chemicals

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Production of δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine by entrapped ACV-synthetase fromStreptomyces clavuligerus

Kadima

Jensen

Pickard

1995

Journal of Industrial Microbiology

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delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine (ACV)-synthetase from Streptomyces clavuligerus was studied under conditions that enabled the reuse of the enzyme. Coupling of ACV-synthetase to DEAE-Trisacryl and aminopropyl-glass resulted in an immobilized enzyme product of little or no catalytic activity. However, an enzyme reactor was designed by physical confinement of partially-purified ACV-synthetase in an ultrafiltration cell. This system was stimulated by phosphoenolpyruvate at lower concentrations of ATP, an effect not observed with purified enzyme. Up to 30% conversion of the limiting substrate, cysteine, to ACV occurred under semi-continuous conditions. Reaction products were investigated as potential inhibitors: AMP was the most inhibitory, but only when used at concentrations in excess of those produced in reaction mixtures. Under a nitrogen atmosphere, both product and enzyme stabilities were greatly improved and the enzyme retained 45-65% of its initial activity after five uses at room temperature during a 24-h period. Extrapolations based on these data suggest that 1.3 g partially purified enzyme (0.13 U g-1) would be capable of producing 411 mg of ACV in a 1-L reaction mixture in this period.

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Cephamycin Production by Streptomyces Clavuligerus

Cited by 5 publications

References 55 publications

Media for Industrial Fermentations

Media for Industrial Fermentations

Lignin Peroxidase and Protease Production by Streptomyces viridosporus T7A in the Presence of Calcium Carbonate

Production of δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine by entrapped ACV-synthetase fromStreptomyces clavuligerus

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