Lignin Peroxidase and Protease Production by Streptomyces viridosporus T7A in the Presence of Calcium Carbonate

Macedo, Jacyara Maria Brito; Gottschalk, Leda Maria Fortes; Bon, Elba P. S.

doi:10.1007/978-1-4612-1604-9_67

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…A clear complex medium is not always advantageous for the production of anthracyclines with actinomycetes [9]. It is, however, a requirement for efficient monitoring of cultivations with traditional methods e.g., optical density or cell dry weight measurements.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

Kiviharju

Leisola

Eerikäinen

2004

J IND MICROBIOL BIOTECHNOL

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Streptomyces peucetius var. caesius is an aerobic bacterium that produces doxorubicin as a secondary metabolite. A mixture design was applied for the screening of suitable complex medium components in the cultivation of S. peucetius var. caesius N47, which is an epsilon-rhodomycinone-accumulating mutant strain. epsilon-Rhodomycinone is a non-glycosylated precursor of doxorubicin. Best growth results were obtained with soy peptone and beef extract. A central composite face-centered (CCF) experimental design was constructed for the investigation of pH, temperature and dissolved oxygen (DO) effects on the cultivation growth phase. Another CCF was applied to the production phase to investigate the effects of aeration, pH, temperature and stirring rate on epsilon-rhodomycinone production. An increase in cultivation temperature increased both cell growth and glucose consumption rate. Best epsilon-rhodomycinone productivities were obtained in temperatures around 30 degrees C. DO control increased all growth phase responses, but aeration in the production phase coupled with pH decrease resulted in rapid epsilon-rhodomycinone decay in the medium. In non-aerated production phases a pH change resulted in better productivity than in experiments without pH change. A pH increase with a temperature decrease seemed most beneficial for productivity. This implies that dynamic control strategies in batch production of epsilon-rhodomycinone could increase the overall process productivity.

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Section: Discussionmentioning

confidence: 99%

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

Kiviharju

Leisola

Eerikäinen

2004

J IND MICROBIOL BIOTECHNOL

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“…is could be attributed to the effect of Ca +2 ions on the morphology of the growing cells and consequently on CO production. It has been reported that addition of Ca +2 ions tends to decrease the probability of formation of pellets and enhances the formation of dispersed mycelia [34]. Moreover, the formed fewer pellets were of reduced size [35,36].…”

Section: Response Surface Experimental Design Using Bbdmentioning

confidence: 94%

Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain

Elsayed

Abdelwahed

2020

BioMed Research International

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Cholesterol oxidase is an alcohol oxidoreductase flavoprotein with wide biotechnological applications. The current work describes the isolation of a potential cholesterol oxidase producing streptomycete from Egyptian soil. The isolated strain produced cholesterol oxidase in submerged culture using a medium containing glucose, yeast extract, malt extract, and CaCO3 with the addition of cholesterol as an inducer. The isolated strain was identified as Streptomyces rochei NAM-19 based on 16S rRNA sequencing and phylogeny. Optimization of cholesterol oxidase production has been carried out using response surface methodology. The Plackett-Burman design method was used to evaluate the significant components of the production medium followed by Box-Behnken experimental design to locate the true optimal concentrations, which are significantly affecting enzyme production. Results showed that the predicted enzyme response could be closely correlated with the experimentally obtained production. Furthermore, the applied optimization strategy increased volumetric enzyme production by 2.55 times (65.1 U/mL) the initial production obtained before medium optimization (25.5 U/mL).

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Lignin Peroxidase and Protease Production by Streptomyces viridosporus T7A in the Presence of Calcium Carbonate

Cited by 2 publications

References 24 publications

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain

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