Centrifuge microextraction coupled with sweeping–MEKC to analyze trace steroid hormones in urine samples

Fang, Huaifang; Yang, Fangxing; Sun, Junling; Tian, Yun; Zeng, Zhaorui; Xu, Ying

doi:10.1016/j.talanta.2011.07.060

Cited by 10 publications

(8 citation statements)

References 37 publications

(45 reference statements)

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“…The method showed good selectivity with seven abused drugs and the LODs were between 200 and 400 ng/L for studied drugs. Fang et al described a method that combines two concentration techniques, offline centrifuge microextraction and online sweeping with MEKC for the determination of trace steroids in urine. The BGE used was 100 mM H 3 PO 4 at pH 2.5 with 50 mM SDS in 30% methanol.…”

Section: Micellar Methodsmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2012

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Sample stacking is a term denoting a multifarious class of methods and their names that are used daily in CE for online concentration of diluted samples to enhance separation efficiency and sensitivity of analyses. The essence of these methods is that analytes present at low concentrations in a large injected sample zone are concentrated into a short and sharp zone (stack) in the separation capillary. Then the stacked analytes are separated and detected. Regardless of the diversity of the stacking electromigration methods, one can distinguish four main principles that form the bases of nearly all of them: (i) Kohlrausch adjustment of concentrations, (ii) pH step, (iii) micellar methods, and (iv) transient ITP. This contribution is a continuation of our previous reviews on the topic and brings an overview of papers published during 2010-2012 and relevant to the mentioned principles (except the last one which is covered by another review in this issue).

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Section: Micellar Methodsmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2012

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“…Sweeping is ideally compatible with other off‐line sample treatment strategies such as LLE and SPE. Fang et al developed a method to analyse trace steroid hormones in urine samples using centrifuge microextraction coupled with sweeping‐MEKC. More than a 500‐fold increase in sensitivity was obtained compared with normal hydrodynamic injection without sample stacking.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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CE has been alive for over two decades now, yet its sensitivity is still regarded as being inferior to that of more traditional methods of separation such as HPLC. As such, it is unsurprising that overcoming this issue still generates much scientific interest. This review continues to update this series of reviews, first published in Electrophoresis in 2007, with updates published in 2009 and 2011 and covers material published through to June 2012. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction and sweeping. Attention is also given to online or inline extraction methods that have been used for electrophoresis.

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“…The supernatant was directly injected in CE-UV with FASI, leading to a limit of detection (LOD) of 0.15 ngmL −1 . CME has also been used for analysis of steroids (e.g., testosterone and progesterone) in urine by micellar electrokinetic chromatography (MEKC) [32]. In 2006, Yangcheng et al proposed directly suspended droplet microextraction (DSDME) to further improve drop stability.…”

Section: Drop Stability Improvementmentioning

confidence: 99%

Microextraction techniques combined with capillary electrophoresis in bioanalysis

2012

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Over the past two decades, many environmentally sustainable sample-preparation techniques have been proposed, with the objective of reducing the use of toxic organic solvents or substituting these with environmentally friendly alternatives. Microextraction techniques (MEs), in which only a small amount of organic solvent is used, have several advantages, including reduced sample volume, analysis time, and operating costs. Thus, MEs are well adapted in bioanalysis, in which sample preparation is mandatory because of the complexity of a sample that is available in small quantities (mL or even μL only). Capillary electrophoresis (CE) is a powerful and efficient separation technique in which no organic solvents are required for analysis. Combination of CE with MEs is regarded as a very attractive environmentally sustainable analytical tool, and numerous applications have been reported over the last few decades for bioanalysis of low-molecular-weight compounds or for peptide analysis. In this paper we review the use of MEs combined with CE in bioanalysis. The review is divided into two sections: liquid and solid-based MEs. A brief practical and theoretical description of each ME is given, and the techniques are illustrated by relevant applications.

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Centrifuge microextraction coupled with sweeping–MEKC to analyze trace steroid hormones in urine samples

Cited by 10 publications

References 37 publications

Contemporary sample stacking in analytical electrophoresis

Contemporary sample stacking in analytical electrophoresis

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Microextraction techniques combined with capillary electrophoresis in bioanalysis

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