CellViCAM—Cell viability classification for animal cell cultures using dark field micrographs

Burgemeister, Sebastian; Nattkemper, Tim Wilhelm; Noll, Thomas; Hoffrogge, Raimund; Flaschel, Erwin

doi:10.1016/j.jbiotec.2010.07.020

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…As such, a variety of techniques have been explored for their potential to monitor it on-line. These include optical techniques which work by measuring either turbidity or nephelometry [2,3], fluorescence-based instruments [4], acoustic resonance densitometry (ARD)-based probes [5] and in-line microscopy [6,7]. Indirect techniques which establish a correlation between some observed quantity such as acid/alkali consumption or oxygen uptake rate and the biomass have also been employed [8].…”

Section: Introductionmentioning

confidence: 99%

Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

Whelan

Murphy

Pearson

et al. 2012

Bioprocess Biosyst Eng

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The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3-2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.

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Section: Introductionmentioning

confidence: 99%

Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

Whelan

Murphy

Pearson

et al. 2012

Bioprocess Biosyst Eng

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“…Nevertheless, errors from light scattering may occur if cells are in contact, since it happens at the cell edges, and therefore the signal will not be proportional to the number of cells [120]. Examples of application of this type of microscopy include examination of bacterial biofilms and their motility [120], cell tracking of amoebae [119], in situ viability classification of S. cerevisiae [115], and viability classification of animal cell cultures [121].…”

Section: Cell Analysis In Solid-state Fermentationsmentioning

confidence: 99%

A Temporal Evolution Perspective of Lipase Production by Yarrowia lipolytica in Solid-State Fermentation

et al. 2022

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Lipases are enzymes that, in aqueous or non-aqueous media, act on water-insoluble substrates, mainly catalyzing reactions on carboxyl ester bonds, such as hydrolysis, aminolysis, and (trans)esterification. Yarrowia lipolytica is a non-conventional yeast known for secreting lipases and other bioproducts; therefore, it is of great interest in various industrial fields. The production of lipases can be carried on solid-state fermentation (SSF) that utilizes solid substrates in the absence, or near absence, of free water and presents minimal problems with microbial contamination due to the low water contents in the medium. Moreover, SSF offers high volumetric productivity, targets concentrated compounds, high substrate concentration tolerance, and has less wastewater generation. In this sense, the present work provides a temporal evolution perspective regarding the main aspects of lipase production in SSF by Y. lipolytica, focusing on the most relevant aspects and presenting the potential of such an approach.

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How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?

Carrondo

Alves

Carinhas

et al. 2012

Biotechnology Journal

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This report highlights the potential of measurement, monitoring, modeling and control (M(3) C) methodologies in animal and human cell culture technology. In particular, state-of-the-art of M(3) C technologies and their industrial relevance of existing technology are addressed. It is a summary of an expert panel discussion between biotechnologists and biochemical engineers with both academic and industrial backgrounds. The latest ascents in M(3) C are discussed from a cell culture perspective for industrial process development and production needs. The report concludes with a set of recommendations for targeting M(3) C research toward the industrial interests. These include issues of importance for biotherapeutics production, miniaturization of measurement techniques and modeling methods.

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CellViCAM—Cell viability classification for animal cell cultures using dark field micrographs

Cited by 7 publications

References 22 publications

Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

A Temporal Evolution Perspective of Lipase Production by Yarrowia lipolytica in Solid-State Fermentation

How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?

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