Cellulolytic enzymes produced by a newly isolated soil fungus Penicillium sp. TG2 with potential for use in cellulosic ethanol production

Jung, You Jin; Park, Jang Min; Heo, Sun-Yeon; Hong, Won-Kyung; Lee, Sung Mok; Oh, Baek Rock; Park, Seung Moon; Seo, Jeong-Woo; Kim, Chul Ho

doi:10.1016/j.renene.2014.10.064

Cited by 35 publications

(12 citation statements)

References 17 publications

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“…(SLL06) is in a group with Penicillium griceofulvum which had the ability to degrade untreated oil palm trunk (OPT) in a solid-state fermentation system (SSF) (Ang et al 2015). Penicillium sp.TG2 has cellulolytic activities which are comparable with those of Trichoderma reesei RUT-C30 which are strains used for commercial cellulase production (Jung et al 2015). Okeke et al (2015) report that Penicillium janthinellum is potential isolate to produce cellulase.…”

Section: Discussionmentioning

confidence: 86%

Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

2017

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Cellulases are the main enzymes in a bioconversion of lignocellulosic biomass. Fungal cellulases have been proven to be better candidates for this process than other microbial cellulases since they secrete free cellulase complex including endoglucanases, cellobiohydrolases, and β-glucosidase. This study was conducted to isolate and screen of cellulolytic fungi from Salacca leaf litter. There were about 12 fungal isolates which were obtained in this research and 8 isolates showed cellulolytic activity. Based on morphological characters, these cellulolytic fungi were identified as belonging to 4 genera, i.e. Penicillium, Aspergillus, Paecilomyces, and Thielaviopsis. Based on clear zone formation, isolate SLL03, SLL06 and SLL10 showed highest cellulolytic activity. Whereas, based on 18S rRNA gene sequence, these isolates were identified as Aspergillums flavus (SLL03), Penicillium sp. (SLL06) Thielaviopsis ethacetica (SLL10). Therefore, these isolates would be good candidates for cellulase producer.

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Section: Discussionmentioning

confidence: 86%

Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

2017

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“…Namun, jika konsentrasi sel sudah meningkat (fase log) maka sintesis enzim respirasi akan digantikan oleh enzim fermentatif. Hal ini menyebabkan jalur respirasi digantikan dengan fermentasi (Jung, et. al., 2015).…”

Section: Hasil Dan Pembahasan Kinerja Proses Sakarifikasi Dan Ferment...unclassified

PRODUKSI BIOETANOL DARI BAGAS SORGUM MANIS MELALUI SAKARIFIKASI DAN FERMENTASI SIMULTAN (SSF) KONVENSIONAL MENGGUNAKAN Trichoderma reesei dan Saccharomyces cerevisiae

Syadiah¹

2021

EDUFORTECH

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Bagas sorgum manis (Sorghum bicolor L. Moench) merupakan hasil samping industri gula dari nira sorgum manis yang dapat digunakan sebagai bahan baku pembuatan bioetanol. Tujuan dari penelitian ini adalah untuk memproduksi bioetanol dari bagas sorgum manis menggunakan Trichoderma reesei dan Saccharomyces cerevisiae. Teknik Sakarifikasi dan Fermentasi Simultan (SSF) konvensional adalah teknik SSF produksi bioetanol dengan aerasi penuh atau tidak direkayasa. Penggunaan teknik SSF memiliki kelebihan jika dibandingkan dengan teknik sakarifikasi dan fermentasi terpisah (SHF), yaitu waktu proses produksi yang dibutuhkan lebih pendek, meningkatkan rendemen, dan laju produksi bioetanol. Produk bioetanol akhir yang dihasilkan sebesar 6.60±0.28 g L-1. Dengan μx maks gabungan sebesar 0.02/jam, nilai Yp/s sebesar 0.17±0.01 g bioetanol/g substrat, dan laju produksi bioetanol sebesar 0.09±0.00 g L-1 jam-1.

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“…3,5-dinitrosalicylic acid (DNS) method was used for spectrophotometric determination of reducing sugar concentration [15]. One international unit of Fpase activity was the amount of enzyme that forms 1 mmol of glucose [16].…”

Section: Enzyme Estimationmentioning

confidence: 99%

Biocomposite Fabrication from Enzymatically Treated Nanocellulosic Fibers and Recycled Polylactic Acid

et al. 2020

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Recycled polylactic acid (PLAr) was reinforced with treated nanocellulosic hemp fibers for biocomposite fabrication. Cellulosic fibers were extracted from hemp fibers chemically and treated enzymatically. Treated nanocellulosic fibers (NCF) were analyzed by Fourier-transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Biocomposite fabrication was done with PLAr and three concentrations of treated NCF (0.1%, 0.25%, and 1% (v/v)) and then studied for thermal stability and mechanical properties. Increased thermal stability was observed with increasing NCF concentrations. The highest value for Young’s modulus was for PLAr + 0.25% (v/v) NCF (250.28 ± 5.47 MPa), which was significantly increased compared to PLAr (p = 0.022). There was a significant decrease in the tensile stress at break point for PLAr + 0.25% (v/v) NCF and PLAr + 1% (v/v) NCF as compared to control (p = 0.006 and 0.002, respectively). No significant difference was observed between treatments for tensile stress at yield.

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Cellulolytic enzymes produced by a newly isolated soil fungus Penicillium sp. TG2 with potential for use in cellulosic ethanol production

Cited by 35 publications

References 17 publications

Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

PRODUKSI BIOETANOL DARI BAGAS SORGUM MANIS MELALUI SAKARIFIKASI DAN FERMENTASI SIMULTAN (SSF) KONVENSIONAL MENGGUNAKAN Trichoderma reesei dan Saccharomyces cerevisiae

Biocomposite Fabrication from Enzymatically Treated Nanocellulosic Fibers and Recycled Polylactic Acid

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