Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

Sari, Siti Lusi Arum; Setyaningsih, Ratna; Wibowo, Novita Fitriatul Aini

doi:10.13057/biodiv/d180355

Cited by 19 publications

(8 citation statements)

References 27 publications

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“…There were two Aspergillus isolates from rhizosphere of raru songal, two isolates from rhizosphere of raru dahanon and one isolate from rhizosphere of raru pulut, while for Penicillium, there were two isolates from rhizosphere of raru songal and each one isolated from rhizosphere of raru dahanon and raru pulut. Aspergillus and Penicillium are cellulolytic fungi which are mostly isolated by previous researchers both from the soil and from leaf litter (Herculano et al 2011;Reddy et al 2014;Torres et al 2014;Sari et al 2017). Molecular identification revealed five Aspergillus isolates consisted of three species, namely, Aspergillus sp.…”

Section: Discussionmentioning

confidence: 98%

Morphological and molecular identification of cellulolytic fungi associated with local raru species

et al. 2019

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Abstract. Elfiati D, Susilowati A, Modes C, Rachmat HH. 2019. Morphological and molecular identification of cellulolytic fungi associated with local raru species. Biodiversitas 20: 2348-2354. This study is aimed to isolate and identify cellulolytic fungi from different local Raru species in Central Tapanuli, North Sumatra, Indonesia. Cellulolytic fungi are fungi that can degrade natural polymer materials such as cellulose. The fungal species were isolated from soil under three local raru species. The samples inoculating (1 mL) from serial dilutions (10-2 to 10-5) on Carboxy Methyl Cellulose (CMC) plates. The plates were incubated at 28 ̊C for one week, then the fungal colonies were observed and pure cultures were maintained. Fungi with cellulolytic activity were characterized by the formation of clear zones around the colonies on CMC media plus congo red (0.1%). The identification of fungi was carried out by molecular identification of the isolated fungi at the species level. The extracted fungal DNA was amplified by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in Gen Bank (NCBI). 20 isolates were successfully isolated and nine isolates had cellulolytic activity. Six fungal species from 20 isolates were identified as: Aspergillus sp., Penicillium sp., Aspergillus terreus, Penicillium mariae-crucis, Aspergillus tanneri and Penicillium citrinum. The results showed that Aspergillus terreus was the most abundant fungus obtained from all the raru stand.

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Section: Discussionmentioning

confidence: 98%

Morphological and molecular identification of cellulolytic fungi associated with local raru species

et al. 2019

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“…Fungi banyak digunakan pada produksi enzim karena memiliki harga yang rendah dengan produktivitas tinggi, tingkat produksi yang cepat dan kemungkinan modifikasi enzim yang mudah. Selulase biasa digunakan dalam produksi tekstil, kertas dan tisu, laundry dan detergent, pertanian, pengobatan dan industri makanan (Jayasekara & Ratnayake, 2019;Sari et al, 2017).…”

Section: Pendahuluanunclassified

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Dhia Salsabila Hakim,

Kasiamdari

2023

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Abstrak: Fungi endofit menjadi perhatian di industri bioetanol karena peranannya dalam sekresi metabolit sekunder berupa enzim selulolitik yang diketahui lebih besar daripada bakteri. Sekresi enzim metabolik pada fungi endofit tergantung pada inang yang ditempatinya. Tanaman herbal dikenal sebagai tempat hidup berbagai fungi endofit. Daun sirih hijau (Piper betle L.) merupakan salah satu tanaman herbal yang dikenal memiliki kandungan metabolit tinggi. Penelitian ini bertujuan untuk mengidentifikasi fungi endofit yang hidup pada P. betle, melakukan uji selulolitik fungi hasil identifikasi serta mengetahui fungi endofit yang memiliki aktivitas selulolitik tebesar. Uji selulolitik dilakukan dengan dua tahap yaitu seleksi dengan Congo Red serta aktivitas selulolitik dihitung dengan kadar gula reduksi. Hasil penelitian didapatkan tiga spesies fungi dari P. betle yaitu Fusarium graminearum, Fusarium oxysporum dan Colletotrichum coccodes. Ketiga sampel yang diujikan menunjukkan positif menghasilkan enzim selulase yang ditandai dengan adanya zona bening di sekitar koloni. Fungi endofit penghasil enzim selulase terbesar adalah spesies F. oxysporum dengan angka 0,033 U/ml.

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“…The screening was done by inoculating the fungal disk onto the SMA plate and incubated at 27ºC for 96 h. The hydrolysis zone around the colony indicates protease activity due to the casein hydrolysis process (Kamath et al 2010;Maitig et al 2018). The enzymatic activity was determined by clear zone formation around the fungal disk (Lusi et al 2017). The enzymatic index (EI) was measured as a semi-quantitative estimate of the enzyme activities, according to the formula below (Coronado-Ruiz et al 2018;Maitig et al 2018).…”

Section: The Enzymatic Activity Assaymentioning

confidence: 99%

Potential of marine sponge-derived fungi in the aquaculture system

2021

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Abstract. Bahry MS, Radjasa OK, Trianto A. 2021. Potential of marine sponge-derived fungi in the aquaculture system. Biodiversitas 22: 2883-2892. Organic waste from aquaculture is one of the triggers of disease outbreaks and a decrease in water quality that urgently needs to be resolved. Indonesia has a high diversity of sponges including their associated microorganisms that potential in the field of biotechnology. This study aimed to determine the enzymatic and anti-vibrio activity of fungi associated with marine sponges and identify potential fungi. The specimen of sponges was collected from Samalona Island, South Sulawesi, Indonesia. The enzymatic and anti-vibrio assay was conducted by using the plug method and the activity was determined by a clear zone around the fungal isolates. Fungal identification was carried out molecularly using universal primers ITS1 and ITS4 and phylogenetic tree analysis. The fungal isolates were screened for the extracellular enzyme activity (amylase, cellulase, protease) and anti-vibrio activity against Vibrio parahaemolyticus, V. harveyi, and V. vulnificus). A total of three fungal isolates have been isolated from the sponge Monanchora sp. Isolate SL 3 SP 3.3 had potential enzymatic activities with Enzymatic Indeks (EI) 3.95±0.17 on amylase, 3.75±0.36 on cellulase, 5.38±0.30 on protease. The highest anti-vibrio activity was obtained against V. harveyi with an inhibition zone diameter of 4.82 ±0.37 mm. The results of fungal identification showed that isolate SL3SP3.3 had a sequence length of 638 bp and was closely related to Trichoderma reesei a.k.a Hypocrea jecorina with a similarity value of 99.69%.

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Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter

Cited by 19 publications

References 27 publications

Morphological and molecular identification of cellulolytic fungi associated with local raru species

Morphological and molecular identification of cellulolytic fungi associated with local raru species

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Potential of marine sponge-derived fungi in the aquaculture system

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