Cellulase Enzyme from <i>Aspergillus niger</i>

Kassim, E. A.

doi:10.1111/j.1348-0421.1982.tb00198.x

Cited by 6 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although protease was not detected on chromogenic media in this study, the analysis of A. niger genome reveals that this species contains serine proteases, aspartic proteases, metalloprotease, amino proteases, putative proteases, miscellaneous [24]. In addition, the production of cellulase enzyme by a selected strain of A. niger isolated from deteriorated wood was reported [25]. P. commune is known to produce pectinase [26].…”

Section: Enzyme Activity Test Resultsmentioning

confidence: 69%

See 1 more Smart Citation

Identification and Characterization of Fungi Contaminated in the Built-In Furniture of an Apartment Home

2019

View full text Add to dashboard Cite

Fungal contamination of built-in furniture is a frequent problem in Korea when new apartment is built. However, domestic information on the contaminating fungi is very limited. This study was conducted to isolate, identify and characterize the fungi of the problem in one of the apartment houses where the fungi were claimed in the built-in furniture before the house owner moves in. Fungi present in the furniture installed in a main room, dress room, and kitchen side were visually and microscopically confirmed and purely isolated on PDA. The isolated fungi were identified by analyzing the morphological characteristics and nucleotide sequence of the ITS, calmodulin gene, and TEF-1a gene. Aspergillus creber, A. niger, A. pseudoglacus, A. ruber, Cladosporium perangustum and Penicillium commune were identified. Four out of the six fungal species were positive for at least one enzyme in six kinds of extracellular enzyme assays. When these four species (A. creber, A. niger, C. perangustum and P. commune) were inoculated onto four kinds of wood chips of furniture materials, they were able to colonize all of the wood chips. Their settlement was better at 95% humidity condition than at 30% humidity condition. Among the four species, C. perangustum caused the darkest discoloration and secreted the most number of extracellular enzymes. The four species were re-isolated from the colonized wood chips and confirmed as the problematic fungi in the built-in furniture.

show abstract

Section: Enzyme Activity Test Resultsmentioning

confidence: 69%

“…There have been studies on wood discoloration by staining fungi [3] and decomposition of wood by wood decay [25]. And building molds have been researched for building damage [5,6].…”

Section: Results Of Inoculation Test On Wood Chipsmentioning

confidence: 99%

Identification and Characterization of Fungi Contaminated in the Built-In Furniture of an Apartment Home

2019

View full text Add to dashboard Cite

show abstract

“…Cellulase activity in monoculture A. niger showed the highest enzyme activity (0.131 U/mL); therefore, A. niger may act as a donor of cellulase enzyme within mixed culture in specific quantities. A. niger produced more extracellular cellulase contain exo-ᵦ-glucanase and ᵦglucosidase that significantly degrade filter paper (Kassim 1982). On the other hand, T. reesei produced more endo-ᵦglucanase, exo-ᵦ-glucanase, and lack of ᵦ-glucosidase (Jamil et al 2009).…”

Section: Purification Of Cellulase Enzymementioning

confidence: 99%

“…Trichoderma reesei more often produces large quantities of endo-ᵦ-glucanase and exo-ᵦ-glucanase, rather than ᵦ-glucoside (Diana et al 2012). Meanwhile, Aspergillus niger found to be the highest producers of ᵦglucoside and endo-ᵦ-glucanase (Kassim 1982). The consortium of Trichoderma reesei and Aspergillus niger will be promising to produce synergistic action of highly extracellular cellulase activity.…”

Section: Introductionmentioning

confidence: 99%

Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

et al. 2019

View full text Add to dashboard Cite

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

show abstract

“…The CBD facilitates cellulose hydrolysis by bringing the CD in contact with the cellulose network, while the CD is the principal site where the catalytic hydrolysis occurs [11,12]. The hydrolysis mechanism is catalysed under acidic conditions, where the catalytic reaction passes through a glycosylenzyme intermediate, subsequently enabling the hydrolysis of the substrate [13,14].…”

Section: Introductionmentioning

confidence: 99%

Facile Cellulase Immobilisation on Bioinspired Silica

et al. 2022

View full text Add to dashboard Cite

Cellulases are enzymes with great potential for converting biomass to biofuels for sustainable energy. However, their commercial use is limited by their costs and low reusability. Therefore, the scientific and industrial sectors are focusing on finding better strategies to reuse enzymes and improve their performance. In this work, cellulase from Aspergillus niger was immobilised through in situ entrapment and adsorption on bio-inspired silica (BIS) supports. To the best of our knowledge, this green effect strategy has never been applied for cellulase into BIS. In situ entrapment was performed during support synthesis, applying a one-pot approach at mild conditions (room temperature, pH 7, and water solvent), while adsorption was performed after support formation. The loading efficiency was investigated on different immobilisation systems by Bradford assay and FTIR. Bovine serum albumin (BSA) was chosen as a control to optimize cellulase loading. The residual activity of cellulase was analysed by the dinitro salicylic acid (DNS) method. Activity of 90% was observed for the entrapped enzyme, while activity of ~55% was observed for the adsorbed enzyme. Moreover, the supported enzyme systems were recycled five times to evaluate their reuse potential. The thermal and pH stability tests suggested that both entrapment and adsorption strategies can increase enzyme activity. The results highlight that the entrapment in BIS is a potentially useful strategy to easily immobilise enzymes, while preserving their stability and recycle potential.

show abstract

Cellulase Enzyme from Aspergillus niger

Cited by 6 publications

References 14 publications

Identification and Characterization of Fungi Contaminated in the Built-In Furniture of an Apartment Home

Identification and Characterization of Fungi Contaminated in the Built-In Furniture of an Apartment Home

Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

Facile Cellulase Immobilisation on Bioinspired Silica

Contact Info

Product

Resources

About