Cellular responses to a nanofibrous environment

Toh, Yi‐Chin; Ng, Shi Ling; Khong, Yuet Mei; Zhang, Xin; Zhu, Yajuan; Lin, Pao-Chun; Te, Chee-Min; Sun, Wanxin; Yu, Hanry

doi:10.1016/s1748-0132(06)70078-0

Cited by 61 publications

(46 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FA sites are proposed to be sites for local translational control (Chicurel et al, 1998;Toh et al, 2006). Protein synthesis components such as mRNAs and ribosomes are shown to localise to FAs (Adereth et al, 2005;Chicurel et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

Zhang

Tee

Heng

et al. 2010

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

SummaryFocal adhesions (FAs) control cell shape and motility, which are important processes that underlie a wide range of physiological functions. FA dynamics is regulated by cytoskeleton, motor proteins and small GTPases. Kinectin is an integral endoplasmic reticulum (ER) membrane protein that extends the ER along microtubules. Here, we investigated the influence of the ER on FA dynamics within the cellular lamella by disrupting the kinectin-kinesin interaction by overexpressing the minimal kinectin-kinesin interaction domain on kinectin in cells. This perturbation resulted in a morphological change to a rounded cell shape and reduced cell spreading and migration. Immunofluorescence and live-cell imaging demonstrated a kinectin-dependent ER extension into the cellular lamella and ER colocalisation with FAs within the cellular lamella. FRAP experiments showed that ER contact with FAs was accompanied with an increase in FA protein recruitment to FAs. Disruption of the kinectin-kinesin interaction caused a reduction in FA protein recruitment to FAs. This suggests that the ER supports FA growth within the cellular lamella. Microtubule targeting to FAs is known to promote adhesion disassembly; however, ER contact increased FA size even in the presence of microtubules. Our results suggest a scenario whereby kinectin-kinesin interaction facilitates ER transport along microtubules to support FA growth.

show abstract

Section: Discussionmentioning

confidence: 99%

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

Zhang

Tee

Heng

et al. 2010

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed many types of cells have been shown to attach to and organize around fibers with diameters smaller than those of the cells, due to interaction of nanoscale cell membrane receptors such as integrins with the nanofibers. [32][33][34] Considering the diameter of the fibers produced by electrospinning for tissue engineering applications, it has been shown by others that endothelial cells adhered and proliferated markedly better on polymer nanofibers (300-1200 nm) than on microfibers (7 mm), 35 and that polymer nanofibers (500-900 nm) promote and regulate specifically the chondrocyte phenotype more efficiently than microfibers (15-20 mm).…”

Section: Figmentioning

confidence: 99%

Chondrogenic Differentiation of Human Mesenchymal Stem Cells on Oriented Nanofibrous Scaffolds: Engineering the Superficial Zone of Articular Cartilage

Wise

Yarin

Megaridis

et al. 2009

Tissue Engineering Part A

220

168

View full text Add to dashboard Cite

Cell differentiation, adhesion, and orientation are known to influence the functionality of both natural and engineered tissues, such as articular cartilage. Several attempts have been devised to regulate these important cellular behaviors, including application of inexpensive but efficient electrospinning that can produce patterned extracellular matrix (ECM) features. Electrospun and oriented polycaprolactone (PCL) scaffolds (500 or 3000 nm fiber diameter) were created, and human mesenchymal stem cells (hMSCs) were cultured on these scaffolds. Cell viability, morphology, and orientation on the fibrous scaffolds were quantitatively determined as a function of time. While the fiber-guided initial cell orientation was maintained even after 5 weeks, cells cultured in the chondrogenic media proliferated and differentiated into the chondrogenic lineage, suggesting that cell orientation is controlled by the physical cues and minimally influenced by the soluble factors. Based on assessment by the chondrogenic markers, use of the nanofibrous scaffold (500 nm) appears to enhance the chondrogenic differentiation. These findings indicate that hMSCs seeded on a controllable PCL scaffold may lead to an alternate methodology to mimic the cell and ECM organization that is found, for example, in the superficial zone of articular cartilage.

show abstract

“…The popularity of nanofibers is demonstrated by the number of reviews focusing on their production, [1][2][3] application, 1,2,4 and interaction with cells. 5 This review discusses information not previously reviewed with regard to the fabrication of polymeric nanofibers in the context of its effect on the physical properties of nanofibrous scaffolds, relevant to tissue engineering, including fiber degradation and control of pore structure and cell infiltration. Finally, bioactive factor loading and applications of polymeric nanofibers in the fields of bone, cartilage, ligament and tendon, cardiovascular, and neural tissue engineering will be presented to demonstrate the utility of nanofibers in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Polymeric Nanofibers in Tissue Engineering

Dahlin

Kasper

Mikos

2011

Tissue Engineering Part B: Reviews

292

198

View full text Add to dashboard Cite

Polymeric nanofibers can be produced using methods such as electrospinning, phase separation, and selfassembly, and the fiber composition, diameter, alignment, degradation, and mechanical properties can be tailored to the intended application. Nanofibers possess unique advantages for tissue engineering. The small diameter closely matches that of extracellular matrix fibers, and the relatively large surface area is beneficial for cell attachment and bioactive factor loading. This review will update the reader on the aspects of nanofiber fabrication and characterization important to tissue engineering, including control of porous structure, cell infiltration, and fiber degradation. Bioactive factor loading will be discussed with specific relevance to tissue engineering. Finally, applications of polymeric nanofibers in the fields of bone, cartilage, ligament and tendon, cardiovascular, and neural tissue engineering will be reviewed.

show abstract

Cellular responses to a nanofibrous environment

Cited by 61 publications

References 127 publications

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

Chondrogenic Differentiation of Human Mesenchymal Stem Cells on Oriented Nanofibrous Scaffolds: Engineering the Superficial Zone of Articular Cartilage

Polymeric Nanofibers in Tissue Engineering

Contact Info

Product

Resources

About