Cellular Localization of PACAP and Its Receptors in the Ovary of the Spotted Ray <i>Torpedo marmorata</i> Risso 1880 (Elasmobranchii: Torpediniformes)

Agnese, Marisa; Valiante, Salvatore; Laforgia, Vincenza; Andreuccetti, Piero; Prisco, Michele De

doi:10.1002/jez.1764

Cited by 8 publications

(2 citation statements)

References 47 publications

(66 reference statements)

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“…Another potential mechanism attributed to AFS and migraine may be related to the involvement of pituitary adenylate cyclase-activating polypeptide (PACAP), an essential target for migraine ( 34 ). In addition, PACAP is involved in the spermatogenesis process of mice ( 35 ), as well as in the production of steroids and the development of ovarian follicles ( 36 ). With premature sexual intercourse, the process of sperm production and the secretion of sex hormones are more frequently activated, hence promoting the increased synthesis of PACAP and thereby inducing migraine.…”

Section: Discussionmentioning

confidence: 99%

Assessing the association between age at first sexual intercourse and migraine: a Mendelian randomization study

Zhu,

Wang,

Wang

et al. 2024

Front. Neurol.

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Background and objectivesAs indicated by observational and genetic variation studies, age at first sexual intercourse (AFS) may be associated with migraine attack, but there is a lack of evidence from real-world studies due to ethical concerns. Therefore, we conducted a Mendelian randomization study to determine the causal relationship between AFS and migraine.MethodsWe extracted instrumental variables from summary data of a genome-wide association study (GWAS) on AFS and migraine and then conducted two-sample Mendelian randomization analyses. GWAS data for AFS and migraine were obtained from 397,338 unrelated individuals (214,547 females and 182,791 males) and 306,314 individuals (18,477 patients and 287,837 control individuals), respectively.ResultsThere was a causal relationship between AFS and risk for migraine (odds ratio (OR) = 0.73, 95% confidence interval (CI) [0.61 to 0.86]), both for migraine with aura (MWA; OR = 0.72, 95% CI [0.58 to 0.89]) and migraine without aura (MOA; OR = 0.66, 95% CI [0.51 to 0.86]). Stratified analyses provided suggestive evidence of the causal relationship between delayed AFS and a decreased risk of migraine for both males (OR = 0.71, 95% CI [0.59 to 0.84]) and females (OR = 0.73, 95% CI [0.61 to 0.89]). Reverse Mendelian randomization did not reveal any effect of migraine on AFS (p > 0.05). No pleiotropy was detected.DiscussionA delayed AFS is a protective factor against migraine (for both MWA and MOA) in both males and females. This causal relationship indicates the presence of extracranial regulatory pathways of migraine.

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Section: Discussionmentioning

confidence: 99%

Assessing the association between age at first sexual intercourse and migraine: a Mendelian randomization study

Zhu,

Wang,

Wang

et al. 2024

Front. Neurol.

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“…Immunohistochemistry was carried out as described elsewhere (Agnese et al, , ). After inactivation of endogenous peroxidase and reducing of non‐specific background, the sections were incubated over night at 4°C with the primary antibodies diluted in normal goat serum.…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning of VIP and distribution of VIP/VPACR system in the testis ofPodarcis sicula

et al. 2014

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Using molecular, biochemical, and cytological tools, we studied the nucleotide and the deduced amino acid sequence of PHI/VIP and the distribution of VIP/VPAC receptor system in the testis of the Italian wall lizard Podarcis sicula to evaluate the involvement of such a neuropeptide in the spermatogenesis control. We demonstrated that (1) Podarcis sicula VIP had a high identity with other vertebrate VIP sequences, (2) differently from mammals, VIP was synthesized directly in the testis, and (3) VIP and its receptor VPAC2 were widely distributed in germ and somatic cells, while the VPAC1 R had a distribution limited to Leydig cells. Our results demonstrated that in Podarcis sicula the VIP sequence is highly preserved and that this neuropeptide is involved in lizard spermatogenesis and steroidogenesis.

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Expression of β-hexosaminidase in the male reproductive system of the lizard, Eutropis carinata. (Reptilia, Squamata) (Schneider, 1801)

2018

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Beta-hexosaminidase (Hex) is the major lysosomal enzyme associated with the event of fertilization. In this study, we have analyzed the distribution of Hex in the testis and the epididymis of the lizard, Eutropis carinata by a polyclonal antibody of β-hexosaminidase isoform (Hex A). Presence of Hex in the epididymis was performed by Western blotting. The result reveals that Hex A is present in the epididymal epithelium, lumen as well as spermatozoa. The anatomical distribution of Hex was studied by immunohistochemical localization. The study reveals that Hex is intensely stained in the epithelium of anterior and middle regions of the epididymis, whereas, posterior epididymal epithelium shows moderate staining. In addition, seminiferous epithelium of the testis shows staining for Hex. But lumen of the testis did not show any reaction for Hex. Further, immunohistochemical localization of Hex on the spermatozoa from the testis and different regions of the epididymis revealed that the Hex from the testis did not show any staining; the epididymal epithelium is moderately localized in the spermatozoa of the anterior region and gradually increases in the intensity in the spermatozoa of the posterior region of the epididymis. This indicates that the Hex is released from the epididymal epithelium and binds to the spermatozoa, and in the lumen, it gradually increases from anterior to the posterior region of the epididymis. The result also suggests that Hex A bound to the epididymal spermatozoa originates from the epididymis and not from the testis. The regional difference in the expression of Hex in the epididymis of the lizard, E. carinata, indicates the possible site of secretion of this enzyme.

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Cellular Localization of PACAP and Its Receptors in the Ovary of the Spotted Ray Torpedo marmorata Risso 1880 (Elasmobranchii: Torpediniformes)

Cited by 8 publications

References 47 publications

Assessing the association between age at first sexual intercourse and migraine: a Mendelian randomization study

Assessing the association between age at first sexual intercourse and migraine: a Mendelian randomization study

Molecular cloning of VIP and distribution of VIP/VPACR system in the testis ofPodarcis sicula

Expression of β-hexosaminidase in the male reproductive system of the lizard, Eutropis carinata. (Reptilia, Squamata) (Schneider, 1801)

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