Molecular cloning of VIP and distribution of VIP/VPACR system in the testis of<i>Podarcis sicula</i>

Agnese, Marisa; Rosati, Luciano; Coraggio, F; Valiante, Salvatore; Prisco, Marina

doi:10.1002/jez.1866

Cited by 18 publications

(24 citation statements)

References 45 publications

(60 reference statements)

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“…Five‐micrometer thick sections of Bouin‐fixed ovary were placed on polylysine glass slides (Menzel‐Glaser, Braunschweig, Germany), dewaxed and rehydrated. The sections were treated as previously reported (Agnese et al, , , b, , b; Rosati et al, , , 2017; Prisco et al, ) with 10 mM citrate buffer pH 6.0 in a microwave for antigen retrieval and then incubated in 2.5% H 2 O 2 in methanol for endogenous peroxidase blocking. Nonspecific background was reduced using normal goat serum (Pierce, Rockford, IL), 1 hr at room temperature.…”

Section: Methodsmentioning

confidence: 99%

The Mussel Mytilus galloprovincialis in the Bay of Naples: New Insights on Oogenic Cycle and Its Hormonal Control

Rosati

Agnese²,

Abagnale

et al. 2019

The Anatomical Record

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The aim of the present article was to investigate the oogenic cycle of Mytilus galloprovincialis sampled in the Bay of Naples, and to immunolocalize 3β‐hydroxysteroid dehydrogenase (3β‐HSD), 17β‐hydroxysteroid dehydrogenase (17β‐HSD), and P450 aromatase, enzymes involved in the synthesis of two sex hormones: testosterone and 17β‐estradiol. We demonstrate that the oogenic cycle starts in late summer‐early fall and continues in early winter when the first event of spawning occurs; other spawning events take place until June, when the ovary is spent and contains a few empty ovarian follicles and numerous somatic cells, that is, adipogranular cells and vesicular connective tissue cells. During the oogenic cycle, apoptotic events occur at the level of oogonia, previtellogenic oocytes, as well as follicle cells; by contrast, necrosis events probably take place in vitellogenic oocytes, which, once degenerated, transfer their content to healthy oocytes. Finally, the present data demonstrate that 3β‐HSD, 17β‐HSD, and P450 aromatase are present in the ovary both during the reproductive and nonreproductive phases. The possible role of these enzymes during the Mytilus galloprovincialis reproductive cycle is discussed. Anat Rec, 302:1039–1049, 2019. © 2019 Wiley Periodicals, Inc.

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Section: Methodsmentioning

confidence: 99%

The Mussel Mytilus galloprovincialis in the Bay of Naples: New Insights on Oogenic Cycle and Its Hormonal Control

Rosati

Agnese²,

Abagnale

et al. 2019

The Anatomical Record

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“…For each reproductive period, ovaries of three different animals were collected and total RNA extracted using TRI‐Reagent (Sigma‐Aldrich, St. Louis, MO) according to the manufacturer's instructions and was evaluated by 1% agarose gel electrophoresis. RNA concentration was measured by determination of absorbance at 260 nm as previously reported (Agnese, Rosati, Prisco, et al, ; Agnese, Rosati, Coraggio, Valiante, & Prisco, ; Coscia, Simoniello, Giacomelli, Oreste, & Motta, ; Rosati et al, ). The RNA was then reverse‐transcribed with QuantiTect Reverse Transcription kit (Qiagen, Hilden, Germany).…”

Section: Methodsmentioning

confidence: 99%

The expression of estrogen receptors during the Mytilus galloprovincialis ovarian cycle

Agnese¹,

Rosati

Prisco³

et al. 2019

J Exp Zool Pt A

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The aim of this paper is to assess, by real‐time polymerase chain reaction and in situ hybridization, the expression of estrogen receptors ER1 and ER2 during the ovarian cycle of Mytilus galloprovincialis. By considering four phases of the reproductive cycle, that is stasis and previtellogenic stage (Stage 0), early vitellogenesis (Stage I), vitellogenesis (Stage II), full‐grown oocyte (Stage III), our investigation demonstrates that the two receptors are differently expressed during the phases investigated of the ovarian cycle: ER1 reaches the highest level at Stage III, whereas ER2 reaches the highest level at Stage II, with ER2 always present at higher levels than ER1. The stage‐dependent receptor expression was recorded within oocytes, follicle cells, and adipogranular cells. No ER1 and ER2 messenger RNAs (mRNAs) were found within vesicular cells. It is to be noted that the ER1 and ER2 expression within the growing oocytes, the follicular, and adipogranular cells overlaps with that of the mRNA for vitellogenin in the same cells, strongly suggesting that in Mytilus, as in vertebrates studied so far, the vitellogenin expression is under the control of estrogens.

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“…Moreover in the lizard, Podarcis sicula , more recently we have demonstrated a different localization of StAR, 3β‐HSD, 17β‐HSD, and 5α‐Red (Rosati, Santillo, et al, ) as well as a different expression and localization of P450 arom (Rosati, Agnese, et al, ), during spermatogenic cycle. This data suggested that, in P. sicula , the specific steroidogenic enzymes, together to other local factors as, pituitary adenylate cyclase activating peptide (PACAP; Rosati, Agnese, et al, ; Rosati et al, ) and vasoactive Intestinal Peptide (VIP; Agnese, Rosati, Coraggio, Valiante, & Prisco et al, ; Agnese et al, ; Rosati, Andreuccetti, et al, ; Rosati et al, ), could control the lizard testis activity. Also in the birds, in particular in zebra finches, were investigated the expression of the sex steroid‐synthesizing enzymes CYP11A1, 3β‐HSD, CYP17, and CYP19 in gonads and adrenals of animal adults (Freking, Nazairians, & Schlinger, ); in the details the authors showed that the ovaries but not the testis may secrete estrogen developmentally and the adrenals may contribute precursors for gonadal steroidogensis.…”

Section: Introductionmentioning

confidence: 98%

Seasonal expression and cellular distribution of star and steroidogenic enzymes in quail testis

et al. 2019

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The quail Coturnix coturnix is a seasonal breeder with a physiological switch on/off of gonadic activity. Photoperiod and temperature are the major environmental factors regulating the spermatogenesis. To more thoroughly comprehend the steroidogenic pathways that govern the seasonal reproductive cycle, we have investigated the localization of StAR protein and steroidogenic enzymes (3β‐HSD, 17β‐HSD, P450 aromatase, and 5α‐Red) as well as androgen and estrogen levels, in the testis of reproductive and nonreproductive quails. We demonstrated that StAR, 3β‐HSD, 17β‐HSD, P450 aromatase, and 5α‐Red were always present in the somatic (Leydig and Sertoli cells) and germ cells (spermatogonia, spermatocytes I and II, spermatids, and spermatozoa). In addition, by western blot analysis, we demonstrated that 17β‐HSD, P450 aromatase, and 5α‐Red showed the highest expression levels during the reproductive testis compared with nonreproductive one. Accordingly, we also found that during the reproductive phase the highest titres of testosterone, 17β‐estradiol, and 5α‐dihydrotestosterone are recorded. In conclusion, our findings demonstrated that in C. coturnix: (a) both somatic and germ cells are involved in the local synthesis of sex hormones; (b) 17β‐HSD, P450 aromatase, and 5α‐Red expressions, as well as testicular androgens and estrogens, increased in reproductive quail testis. This study strongly indicates that the steroidogenic process in quail testis exhibits seasonal changes with the promotion of both androgenic and estrogenic pathways in the reproductive period, suggesting their synergic mechanism in the spermatogenesis regulation.

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Molecular cloning of VIP and distribution of VIP/VPACR system in the testis ofPodarcis sicula

Cited by 18 publications

References 45 publications

The Mussel Mytilus galloprovincialis in the Bay of Naples: New Insights on Oogenic Cycle and Its Hormonal Control

The Mussel Mytilus galloprovincialis in the Bay of Naples: New Insights on Oogenic Cycle and Its Hormonal Control

The expression of estrogen receptors during the Mytilus galloprovincialis ovarian cycle

Seasonal expression and cellular distribution of star and steroidogenic enzymes in quail testis

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