Cellular localization of inhibin mRNA in the bovine ovary by in-situ hybridization

Torney, A. H.; Hodgson, Yvonne; Forage, R G; Kretser, D. M. de

doi:10.1530/jrf.0.0860391

Cited by 58 publications

(54 citation statements)

References 26 publications

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“…In primates, circulating levels of inhibin A and P 4 manifested concordant changes such that both exhibited rise and fall during the luteal phase and both decreased following lutectomy which confirms the source of inhibin secretion during the luteal phase [39,40,45]. Inhibin A biosynthesis in CL appears to be present only in higher primates since expression of inhibin-α subunit has been reported to be turned off following luteinization in rodent and bovine species [46][47][48].…”

Section: B Inhibins In Female Gonadsmentioning

confidence: 97%

New targets for old hormones: inhibins clinical role revisited [Review]

2011

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Abstract. Inhibins are gonadal peptide hormones belonging to the transforming growth factor-β (TGF-β) superfamily that regulate the pituitary follicle stimulating hormone (FSH) secretion by negative feedback mechanisms. It is evident that the understanding of inhibins function in the hypothalamic-pituitary-gonadal axis will provide insights into physiology and pathology of the gonadal function. In recent years, a great deal of attention has been focussed on clinical relevance of measuring circulating inhibins in normal and disease state. The past few years also have witnessed the emergence and discovery of extra pituitary action of inhibins that might provide further insights into the underlying diseases like cancer especially in the reproductive axis and various other new endocrine target organs. In this review after systematic analysis of literature, we discuss briefly the known and recent advances in function of these hormones highlighting also its structure, production and mechanisms of signal transduction. Also this review discusses about the physiological relevance of inhibin association in the normal function to the development of reproductive cancers. Finally, we describe evidence from various emerging studies that inhibins make an important contribution to other physiological functions apart from reproduction which reveals new endocrine target organs of inhibins. The emerging view is inhibin participates in multiple ways to regulate the function in different cell types and still complete repertoire of its actions is under investigation. Key words: Inhibin, Transforming growth factor, Ovarian cancer, Activins, BetaglycanMAMMAliAn reproduction is intricate, highly ordered and is coordinated by assorted neural, neuroendocrine, endocrine, autocrine and paracrine cell-cell communication pathways. Many of the pioneering discoveries meticulously demonstrated the roles of hypothalamic gonadotropin releasing hormone (GnRH) that stimulates pituitary gonadotropins such as follicle stimulating hormone (FSH) and leutinizing hormone (LH), which directly control gonadal functions. Subsequent breakthroughs in the advancement of molecular biology and in understanding signal transduction mechanisms suggested involvement of various cytokines and growth factors at each level of the hypothalamic-pituitary-gonadal axis in the paracrine/autocrine control of different programmed reproductive events. Members include inhibins and activins, belonging to the transforming growth factor (TGF-β) super family and follistatin (FS), a structurally distinct though functionally related molecule. It has been more than 70 years since the identification of negative feedback control of pituitary FSH secretion by inhibins. These factors also were shown to act in autocrine, endocrine or paracrine manner within the reproductive tissues, but the identification of specific pathways in different cellular context along the gonadal axis is still quite unclear and should thus be further investigated. The discovery process of extra pituitary action of ...

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Section: B Inhibins In Female Gonadsmentioning

confidence: 97%

New targets for old hormones: inhibins clinical role revisited [Review]

2011

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“…Much of our understanding regarding inhibin regulation relies on in situ hybridization studies in which the mRNAs for the individual inhibin subunits ( , A , and B ) have been localized to individual follicles using sequencespecific probes (Meunier et al 1988, Woodruff et al 1988, Torney et al 1989, Engelhardt et al 1993, Fraser et al 1993, Jih et al 1993, Roberts et al 1993, Garrett et al 2000. It is clear that follicle-stimulating hormone (FSH) stimulates the synthesis of all three inhibin subunits in granulosa cells of antral follicles, and inhibits all three inhibin subunits in preovulatory follicles (Woodruff et al 1987, Richards & Hedin 1988, Meunier et al 1989, Rivier et al 1989, Woodruff & Mayo 1990, Michel et al 1991, Eramaa et al 1994, Drummond et al 2000.…”

Section: Introductionmentioning

confidence: 99%

Photoperiod-dependent regulation of inhibin in Siberian hamsters: II. Regulation of inhibin production and secretion by pregnant mare serum gonadotropin

Kenny

Bernard²,

Horton

et al. 2002

Journal of Endocrinology

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Inhibin production differs in ovaries of Siberian hamsters (Phodopus sungorus) exposed to long days (LD) or short days (SD). We believe that seasonal differences in serum follicle-stimulating hormone contribute to this difference. However, given the profound photoperiodic differences in follicle maturation, serum gonadotropins alone may not account for all of the observed differences in inhibin processing. To test this hypothesis, we challenged LD and SD female hamsters with exogenous gonadotropins. While both groups responded with increased inhibin expression, the effects were muted in ovaries of SD females and there was no evidence of ovulation in these animals. These data indicate that the ovaries of SD females are not immediately equipped to respond to gonadotropin stimulation. More generally, these data suggest that photoperiodic history affects ovarian inhibin production and secretion in response to gonadotropins.

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“…In the porcine ovaries, follicular fluid inhibin level also decreased during follicular growth and was not related to follicular fluid estradiol-17β (E2) level [19]. In the bovine ovaries, inhibin has been detected immunohistochemically only in follicular granulosa cells [20][21][22], but inhibin mRNA was detected in both granulosa cells and theca interna cells by in situ hybridization [5]. In these studies yielding inconsistent and conflicting results, investigators did not strictly classify the follicular stage, especially the histological changes occurring in the follicles at each stage of follicular growth and atresia.…”

mentioning

confidence: 99%

“…nhibin is a glycoprotein with a molecular weight of 32 kDa and a heterodimer consisting of an α-subunit (20 kDa) and one of two β-subunits (βA or βB; 13 kDa) linked by disulfide bridges, and the α-and β-subunits are produced by different genes [1][2][3][4][5][6][7][8][9][10]. Inhibin is mainly produced by the granulosa cells of the ovaries, and its major physiological role is specific suppression of secretion of folliclestimulating hormone (FSH) from the anterior pituitary gland [1][2][3][4][5][6][7][8][9][10][11][12][13][14].…”

mentioning

confidence: 99%

“…Inhibin is mainly produced by the granulosa cells of the ovaries, and its major physiological role is specific suppression of secretion of folliclestimulating hormone (FSH) from the anterior pituitary gland [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Many studies of inhibin activity estimated by radioimmunoassay and/or bioassay have demonstrated that growing healthy follicles produce and secrete much more inhibin into follicular fluid than atretic follicles [4,5,7,9,[15][16][17][18].…”

mentioning

confidence: 99%

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Stage-Specific Distributions of Inhibin .ALPHA.-subunit in Bovine Ovarian Follicles and Various Hormones Levels in Follicular Fluid.

Yoshimura¹,

Hondo²,

Murabayashi³

et al. 1999

Journal of Reproduction and Development

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Abstract. The changes in localization of inhibin α-subunit in bovine ovarian follicles during follicular development were assessed by immunohistochemical staining with monoclonal anti-inhibin α-subunit antibody. The changes in inhibin, estradiol-17β (E2), progesterone (P) and testosterone (T) levels in the follicular fluid during follicular development were also investigated. The follicles obtained from the bovine ovaries in mid-luteal stage were histologically classified into 7 stages. Stages I, II and III were growing healthy follicles, and stages IV, V, VI and VII were atretic follicles. In both growing and atretic follicles, inhibin α-subunit were histochemically demonstrated in the granulosa cells. Strong or moderate immunostaining for inhibin α-subunit were found only in the granulosa cells of stages II and III follicles (the strongest staining was found in stage III), and trace staining was seen in a small number of theca interna cells of stages II and III follicles. The granulosa cells of early atretic follicles (stage IV) also have the ability to produce and secrete inhibin, but finally they lost the ability. No positive staining was demonstrated in any cells of progressed atretic follicles (stage V, VI and VII). Inhibin level in the follicular fluid was higher in the growing follicles (highest level at stage II) than in the atretic follicles. Follicular fluid E2 level increased from stages I to III, but decreased from stage IV onward. The P levels remained low from stages I to VI, but increased only in stage VII. The T levels in stages III and VII were lower than in the other stages. Follicular fluid inhibin level were related to E2 level but not P or T levels. In conclusion, the inhibin α-subunit is produced by follicular granulosa cells in growing follicles of the bovine ovaries, and inhibin may stimulate E2 secretion in growing but not in atretic follicles.

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Cellular localization of inhibin mRNA in the bovine ovary by in-situ hybridization

Cited by 58 publications

References 26 publications

New targets for old hormones: inhibins clinical role revisited [Review]

New targets for old hormones: inhibins clinical role revisited [Review]

Photoperiod-dependent regulation of inhibin in Siberian hamsters: II. Regulation of inhibin production and secretion by pregnant mare serum gonadotropin

Stage-Specific Distributions of Inhibin .ALPHA.-subunit in Bovine Ovarian Follicles and Various Hormones Levels in Follicular Fluid.

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