Cellular hydrophobicity of Listeria monocytogenes involves initial attachment and biofilm formation on the surface of polyvinyl chloride

Abstract

Aims: To clarify the cellular properties of Listeria monocytogenes involved in adhesion to and biofilm formation on polyvinyl chloride, a widely used material in the food manufacturing process. Methods and Results: A significant correlation between the ability of initial adherence to and biofilm formation on PVC was observed for 24 L. monocytogenes strains (Spearman rank‐correlation coefficient, rs = 0·89). The swimming motility assay revealed no relationship between initial adherence and motility of L. monocytogenes. The microbial adhesion to solvent assay revealed an interaction of L. monocytogenes cells with nonpolar solvents, and a significant correlation was also observed between the degree of interaction with nonpolar solvents and initial adherence to PVC (rs = 0·87 and rs = 0·84, between initial adherence and affinities to decane and hexadecane, respectively). Conclusions: Results indicate that cellular hydrophobicity of L. monocytogenes is an important property involved in the initial adherence to and biofilm formation on PVC. Significance and Impact of Study: This study clarified the factors involved in the adherence to and biofilm formation ability of L. monocytogenes strains with PVC.

Characterization of Biofilms From Selected Synthetic Materials Used in Water Distribution System

“…Moreover, in his research Djodjevic concluded that on polyvinyl chloride there was no positive correlation between cell motility and biofilm formation (Djordjevic et al, 2002). The adhesion on PVC is closely related to the hydrophobicity of the cells (Takahashi et al, 2010). On the basis of the results results, it was found that the metabolic profile of a biofilm on PVC indicated the ability It was confirmed that a metabolic profile of a biofilms formed on various materials indicates specific metabolic activity which may be inversely proportional to an increase of biofilm mass (Simoes et al, 2007).…”

Exposure of Escherichia coli ATCC 12806 to Sublethal Concentrations of Food-Grade Biocides Influences Its Ability To Form Biofilm, Resistance to Antimicrobials, and Ultrastructure

“…an increase in bacterial ability to form biofilms on polystyrene surfaces (11,26,35). However, controversial results on the effect of cell surface hydrophobicity on biofilm formation on cell contact surfaces have been reported (24).…”

The effect of polyesterurethane belt surface roughness on Listeria monocytogenes biofilm formation and its cleaning efficiency

“…ATCC19114 represents a strong strain, as it can attach to the surface and rapidly form a dense biofilm, while ATCC51782 represents a weak strain, which attaches and forms looser biofilm (Takahashi et al, 2010). Both strains were kept on trypticase soy agar (TSA, Becton Dickinson, Sparks, MD, USA) slants at 4 C and were activated by two successive transfers in a trypticase soy broth (TSB, Becton Dickinson, Sparks, MD, USA) containing 0.6% yeast extract (Becton Dickinson) (TSB-YE) at 30 C for 24 h. The cultures were incubated until the late exponential phase, and were then adjusted to obtain cell concentrations of approximately 10 9 CFU/ml (optical density at 600 nm [OD 660 ] ¼ 0.02).…”