Characterization of Biofilms From Selected Synthetic Materials Used in Water Distribution System

Biedroń, Izabela; Traczewska, T. M.; Konieczny, Tomasz; Płaza, Grażyna

doi:10.12911/22998993/67850

Cited by 6 publications

(18 citation statements)

References 25 publications

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“…3). It was a strain which showed very high antibiotic resistance [28]. No impact of silver nanoparticles was observed for strain 3-Kocuria rhizophila or Psychrobacter sp.…”

Section: Resultsmentioning

confidence: 96%

“…In the case of two strains: Bacillus thuringiensis (5) and Moraxella osloensis or Enhydrobacter aerosaccus (2), where there was no observed the reduction in biofilm formation. The strains are Gram positive and produce significant amount of EPS composed mainly from proteins [28]. Also strain belong to the genus Microccocus (10) showed no significant decrease of biofilm formation.…”

Section: Resultsmentioning

confidence: 99%

“…Morphological characterization (colony colour, shape and size) was recorded on selective media and biochemical characterization was tested according to API Zym test [28]. The identification of selected strains was carried out by the sequencing and comparative analysis of 16S rDNA,.…”

Section: Bacterial Strainsmentioning

confidence: 99%

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TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles

2017

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Abstract. Among metal nanoparticles, silver nanoparticles are a widely used in various life sectors such as in biomedical applications, air and water purification, food production, cosmetics, garments and in various household products. There are several methods for production of silver nanoparticles. Generally, silver nanoparticles can be prepared by chemical methods such as chemical reduction and electrochemical techniques, physical methods, and biological methods such as the use of microorganisms. The biological route of synthesis provides a great diversity in choice for its raw materials such as bacteria, algae, fungi and plants. The aim of the study was to evaluate the tetrazolium/formazan test as a method to determine antibiofilm activity of biological synthetized silver nanoparticles. In this study Bacillus subtilis grown on brewery effluent and produced biosurfactant was used for silver nanoparticles (Ag-NPs) synthesis. The culture supernatants were used in synthesis of Ag-NPs. The formation of nanoparticles accompanied by colour changes of the used reaction system was confirmed by UV-vis spectroscopy. The bacteria isolated from the biofilm of water supply system were used in the evaluation of the antibiofilm activity of biologically synthetized Ag-NPs. To compare the results the commonly used crystal violet assay (CV) for biofilm analysis was applied.

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“…3). It was a strain which showed very high antibiotic resistance [28]. No impact of silver nanoparticles was observed for strain 3-Kocuria rhizophila or Psychrobacter sp.…”

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

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TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles

2017

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“…For the culture of the biofilm we have used a CDC reactor from Biosurface Technologies company (Fig 1A).The contents of the reactor was a synthetic tap water inoculated with a mixture of 11 strains from our own collection [10]. Three segments of pipes made of plastic (polyethylene, polypropylene, polyvinyl chloride, polybutylene) ( Figure 1B) and a measuring sensor ( Figure 1C) were placed in the reactor.…”

Section: Methodsmentioning

confidence: 99%

The application of impedance measurement to assess biofilm development on technical materials used for water supply system construction

2017

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Abstract. The lack of biological stability of water which is introduced into the network, leads primarily to its secondary contamination during transport to the consumer. The water that is biologically unstable creates ideal conditions for colonization of the inner surface of pipelines by microorganisms and adhesion of their products (biocorrosion). The studies was conducted using the identified microorganisms isolated from the water supply network which accounted inocula in continuous culture of biofilm in CDC reactor. As a result of studies it was revealed the presence of biofilm formed on different materials polyethylene, polypropylene, polyvinyl chloride, polybutylene. Microbiological biodiversity of organisms inhabiting a biofilm of the diversity of nucleic acids was used. It was observed the amount of the psychrophilic bacteria oscillation in the effluent from the reactor. It was also determined the affinity of various bacteria to the plastic through adhesion measurement using impedance spectroscopy. For impedance measurements apparatus SIGNAL RECOVERY 7280 DSP LOCK-IN AMPLIFIER was used, recording impedance components (real and imaginary). The results will allow for the creation of biosensor systems that can be used in predicting health risks in connection with drinking water and taking corrective actions.

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“…Taxonomic identification and characterization of these strains have been described previously by Biedron et al [12]. The strains were used to test the antibacterial and antibiofilm properties of AgNPs synthesized biologically and chemically.…”

Section: Bacterial Strains Used In the Experimentsmentioning

confidence: 99%

The effect of biologically and chemically synthesized silver nanoparticles (AgNPs) on biofilm formation

et al. 2017

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Abstract. Bionanotechnology has emerged up as integration between biotechnology and nanotechnology for developing biosynthetic and environmental-friendly technology for synthesis of nanomaterials. Different types of nanomaterials like copper, zinc, titanium, magnesium, gold, and silver have applied in the various industries but silver nanoparticles have proved to be most effective against bacteria, viruses and eukaryotic microorganisms. The antimicrobial property of silver nanoparticles are widely known. Due to strong antibacterial property silver nanoparticles are used, e.g. in clothing, food industry, sunscreens, cosmetics and many household and environmental appliances. The aim of the study was to compare the effect of silver nanoparticles (AgNPs) synthesized biologically and chemically on the biofilm formation. The biofilm was formed by the bacteria isolated from the water supply network. The commonly used crystal violet assay (CV) was applied for biofilm analysis. In this study effect of biologically synthesized Ag-NPs on the biofilm formation was evaluated.

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Characterization of Biofilms From Selected Synthetic Materials Used in Water Distribution System

Cited by 6 publications

References 25 publications

TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles

TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles

The application of impedance measurement to assess biofilm development on technical materials used for water supply system construction

The effect of biologically and chemically synthesized silver nanoparticles (AgNPs) on biofilm formation

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