Cellular cytotoxic response induced by highly purified multi-wall carbon nanotube in human lung cells

Tsukahara, Tamotsu; Haniu, Hisao

doi:10.1007/s11010-011-0739-z

Cited by 54 publications

(61 citation statements)

References 27 publications

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“…Carbon nanotubes have greatly advanced these fields. Similarly as other nanoparticles, carbon nanotubes can be readily taken up by cells and are noncytotoxic within a certain concentration limit if suitably functionalized [25] [26]. The main finding of this study was that HTT2800 has a significant effect on intracellular lipid accumulation in C2C12 cells.…”

Section: Discussionmentioning

confidence: 68%

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Tsukahara¹,

Haniu²

2011

Biochemical and Biophysical Research Communications

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In this report, we sought to elucidate whether multiwall carbon nanotubes are involved in the modulation of the proliferation and differentiation of the skeletal muscle cell line C2C12. Skeletal muscle is a major mass peripheral tissue that accounts for 40% of total body weight and 50% of energy consumption. We focused on the differentiation pathway of myoblasts after exposure to a vapor-grown carbon fiber, HTT2800, which is one of the most highly purified carbon nanotubes.This treatment leads in parallel to the expression of a typical adipose differentiation program. We found that HTT2800 stimulated intracellular lipid accumulation in C2C12 cells. We have also shown by quantified PCR analysis that the expression of adipose-related genes was markedly upregulated during HTT2800 exposure. Taken together, these results suggest that HTT2800 specifically converts the differentiation pathway of C2C12 myoblasts to that of adipoblast-like cells.

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Section: Discussionmentioning

confidence: 68%

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Tsukahara¹,

Haniu²

2011

Biochemical and Biophysical Research Communications

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“…These results were in accordance with a previous report, in which a similar effect was observed in the BEAS-2 cell line. 23 By contrast, in the case of THP-1 cells, MWCNTs adhered around the cells, but were not ingested. This response was similar to that previously observed in U937 cells.…”

Section: Resultsmentioning

confidence: 95%

“…22 The objective of the present study was to confirm whether the expression of DJ-1 in U937 cells is universal. We recently also reported that exposure of BEAS-2B cells (derived from human bronchial epithelium) to HTT2800 proved cytotoxic, 23 causing arrest of proliferation. One of the most significant differences between U937 and BEAS-2B cells is their surface adhesiveness.…”

Section: Resultsmentioning

confidence: 97%

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Haniu¹,

Tsukahara²,

Matsuda³

et al. 2011

IJN

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Background In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. Methods Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. Results We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. Conclusion These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs.

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“…Similarly, exposure of nano-sized carbon black by pulmonary instillation exacerbated the atherosclerotic lesions in the aorta of mice susceptible to the development of AS 29) . A number of possible mechanisms have been proposed to explain the adverse health effects of MWCNTs, including their abilities to induce oxidative stress 15,30) and inflammation 31) , or induce platelet aggregation and vascular thrombosis 32) . Additionally, turbulences in blood vessels, especially in the endo- VWF β -actin VWF 0 µ g/ml 2 µ g/ml 5 µ g/ml 10 µ g/ml 20 µ g/ml 50 µ g/ml F thelium, could contribute significantly to the pathogenesis of AS.…”

Section: Discussionmentioning

confidence: 99%

Intravenous Administration of Multi‐walled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats

Yang

Shen

et al. 2012

Journal of Occupational Health

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Intravenous Administration of Multiwalled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats: Yu‐Ying XU, et al. Zhejiang University School of Medicine, China— Background Carbon nanotubes (CNTs) have many potential applications, including as delivery systems for a variety of diagnostic or therapeutic agents. However, it has been suggested that exposure to carbon nano‐materials may be a risk for the development of vascular diseases due to its impact on the vascular endothelium. Materials and Methods Male Sprague‐Dawley rats (180−200 g) were used to generate an atherosclerosis (AS) model, and the effect of intravenous administration of multi‐walled carbon nanotubes (MWCNTs) on AS was studied. To further understand the underlying mechanisms, the effects of exposure of human umbilical vascular endothelial cells (HUVECs) to MWCNTs were examined. Results Exposure to 200 μg/kg MWCNTs aggravated AS in this model. In addition, exposure to 50, 100 and 200 μg/kg MWCNTs increased the calcification of the aorta in the model. Short‐term exposure also revealed that 200 μg/kg MWCNTs injured the endothelium in the aorta. MWCNTs disrupted the endothelial tight junction and induced endothelial cell death. Conclusion The results demonstrated that MWCNTs could induce structural and functional changes in the endothelium, probably through vascular endotheliocyte injury, which eventually affected the development of AS in SD rats.

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Cellular cytotoxic response induced by highly purified multi-wall carbon nanotube in human lung cells

Cited by 54 publications

References 27 publications

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Intravenous Administration of Multi‐walled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats

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