Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Tsukahara, Tamotsu; Haniu, Hisao

doi:10.1016/j.bbrc.2011.02.090

Cited by 22 publications

(22 citation statements)

References 33 publications

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“…Our results indicate that different physical properties of the carbon nanomaterials used were recognized by the cell membrane, resulting in distinctive responses. Our previous finding that HTT2800 converted the differentiation pathway of C2C12 myoblasts into that of adipoblast-like cells 26 further supports this hypothesis. DJ-1 exists abundantly in the human body, with extremely diverse functions.…”

Section: Resultssupporting

confidence: 63%

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Haniu¹,

Tsukahara²,

Matsuda³

et al. 2011

IJN

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Background In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. Methods Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. Results We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. Conclusion These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs.

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Section: Resultssupporting

confidence: 63%

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Haniu¹,

Tsukahara²,

Matsuda³

et al. 2011

IJN

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“…Abbreviations: VGCF, vapor-grown carbon fiber; ROS, reactive oxygen species. 35 On the other hand, the reasons why these cells adhered in the aggregated SB were an observed slight decrease in cell permeability and increased IL-8 secretion. It may be that the agglomeration of SB was entirely small and had little effect.…”

mentioning

confidence: 99%

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Haniu

Matsuda²,

Kim³

et al. 2011

IJN

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We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.

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“…81 They have been shown to have pluripotent mesenchymal precursor cell characteristics. Tsukahara et al 78 tested the ability of one of the most highly purified MWCNTs (HTT2800) to modulate the proliferation and differentiation of the skeletal muscle cell line, C2C12. The major finding of this study was that HTT2800 greatly influenced intracellular lipid accumulation in C2C12 cells.…”

Section: Other Cell Linesmentioning

confidence: 99%

“…Overall, these findings demonstrate, for the first time, that HTT2800 promotes the transition from a myogenic lineage to an adiposelike lineage, partly determined by CD36 overexpression. 78 …”

Section: Other Cell Linesmentioning

confidence: 99%

Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface

Mocan¹,

Ilie²,

Mocan³

2012

IJN

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During the last decade, due to advances in functionalization chemistry, novel nanobiomaterials with applications in tissue engineering and regenerative medicine have been developed. These novel materials with their unique physical and chemical properties are bioactive hierarchical structures that hold great promise for future development of human tissues. Thus, various nanomaterials are currently being intensively explored in the directed differentiation of stem cells, the design of novel bioactive scaffolds, and new research avenues towards tissue regeneration. This paper illustrates the latest achievements in the applications of nanotechnology in tissue engineering in the field of regenerative medicine.

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Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Cited by 22 publications

References 33 publications

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface

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