DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Haniu, Hisao; Tsukahara, Tamotsu; Matsuda, Yoshikazu; Usui, Yuki; Aoki, Katsumi; Shimizu, Masayuki; Ogihara, Nobuhide; Hara, Kazuo; Takanashi, Seiji; Okamoto, Masato; Ishigaki, Norio; Nakamura, Kōichi; Kato, Hiroyuki

doi:10.2147/ijn.s25471

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…The neuroblast was added in order to evaluate neurotoxicity because carbon particles able to cross the blood-brain barrier are known to affect the central nervous system 28 and MWCNTs are known to influence a protein associated with neurons. 21,29 We also examined the effect of MWCNTs on cells at different states of differentiation, and the relationship between cytotoxicity and the cellular uptake of MWCNTs.…”

Section: Discussionmentioning

confidence: 99%

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Haniu

Matsuda²,

Kim³

et al. 2011

IJN

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We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.

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Section: Discussionmentioning

confidence: 99%

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Haniu

Matsuda²,

Kim³

et al. 2011

IJN

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“…The resulting oxidative damage due to ROS causes damage to DNA, proteins, and lipids and the activation of cell signaling pathways [ 4 , 27 ]. Our previous study focused on the acute toxicity of a vapor-grown CNT, HTT2800, which is a highly purified MWCNT that was prepared using high-temperature treatment [ 22 , 28 ]. Shape of HTT2800 is characterized by a high aspect ratio, a diameter of 100 to 150 nm, and a length of 10 to 20 µm [ 28 ].…”

Section: Cnt Cytotoxicity and Impuritiesmentioning

confidence: 99%

“…Our previous study focused on the acute toxicity of a vapor-grown CNT, HTT2800, which is a highly purified MWCNT that was prepared using high-temperature treatment [ 22 , 28 ]. Shape of HTT2800 is characterized by a high aspect ratio, a diameter of 100 to 150 nm, and a length of 10 to 20 µm [ 28 ]. Since the iron catalyst was removed from HTT2800 in an argon atmosphere, only a very low concentration (<20 ppm) of the iron-based material remained [ 4 ].…”

Section: Cnt Cytotoxicity and Impuritiesmentioning

confidence: 99%

The Role of Autophagy as a Mechanism of Toxicity Induced by Multi-Walled Carbon Nanotubes in Human Lung Cells

Tsukahara

Matsuda

Haniu

2014

IJMS

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Carbon nanotubes (CNTs) are promising nanomaterials having unique physical and chemical properties, with applications in a variety of fields. In this review, we briefly summarize the intrinsic properties of highly purified multi-walled CNTs (MWCNTs, HTT2800) and their potential hazardous effects on intracellular and extracellular pathways, which alter cellular signaling and impact major cell functions such as differentiation, reactive oxygen species (ROS) production, apoptosis, and autophagy. A recent study suggested that the induction of autophagy by CNTs causes nanotoxicity. Autophagy was recently recognized as a critical cell death pathway, and autophagosome accumulation has been found to be associated with exposure to CNTs. Although autophagy is considered as a cytoprotective process, it is often observed in association with cell death, and the relationship between autophagy and cell death remains unclear. Our recent study suggests that the levels of autophagy-related genes (LC3B) and autophagosome formation are clearly up-regulated, along with an increase in numbers of autophagosome vacuoles. This review highlights the importance of autophagy as an emerging mechanism of CNT toxicity.

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“…Multiwalled carbon nanotubes (MWCNTs) have the ability to promote stem cells' differentiation towards bone cells and enhance bone formation [35][36][37]. Due to their nanometer scale size, with a very high strength to weight ratio, they are very attractive as a nanoadditive to improve mechanical properties of polymers, including PLA fibres [38][39][40].…”

Section: Introductionmentioning

confidence: 99%

PLA-Based Hybrid and Composite Electrospun Fibrous Scaffolds as Potential Materials for Tissue Engineering

Magiera

Markowski

Menaszek

et al. 2017

Journal of Nanomaterials

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The aim of the study was to manufacture poly(lactic acid)-(PLA-) based nanofibrous nonwovens that were modified using two types of modifiers, namely, gelatin-(GEL-) based nanofibres and carbon nanotubes (CNT). Hybrid nonwovens consisting of PLA and GEL nanofibres (PLA/GEL), as well as CNT-modified PLA nanofibres with GEL nanofibres (PLA + CNT/GEL), in the form of mats, were manufactured using concurrent-electrospinning technique (co-ES). The ability of such hybrid structures as potential scaffolds for tissue engineering was studied. Both types of hybrid samples and one-component PLA and CNTs-modified PLA mats were investigated using scanning electron microscopy (SEM), water contact angle measurements, and biological and mechanical tests. The morphology, microstructure, and selected properties of the materials were analyzed. Biocompatibility and bioactivity in contact with normal human osteoblasts (NHOst) were studied. The coelectrospun PLA and GEL nanofibres retained their structures in hybrid samples. Both types of hybrid nonwovens were not cytotoxic and showed better osteoinductivity in comparison to scaffolds made from pure PLA. These samples also showed significantly reduced hydrophobicity compared to one-component PLA nonwovens. The CNT-contained PLA nanofibres improved mechanical properties of hybrid samples and such a 3D system appears to be interesting for potential application as a tissue engineering scaffold.

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DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

Cited by 7 publications

References 23 publications

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

The Role of Autophagy as a Mechanism of Toxicity Induced by Multi-Walled Carbon Nanotubes in Human Lung Cells

PLA-Based Hybrid and Composite Electrospun Fibrous Scaffolds as Potential Materials for Tissue Engineering

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