Cellular behavior of L929 and MG-63 cells cultured on electrospun nanofibers of chitosan with different degrees of phosphorylation

Abstract

Phosphate groups chemically grafted onto polymer substrates can be used as biomimetic analogs for in vitro studying of function of biomacromolecules and also as tissue substitutes in clinical conditions of organ loss. Despite this inspiration, studies correlating effect of degree of phosphate grafting of a polymer on fabrication and biological properties of polymers are lacking. In this work, N-methylene phosphonic chitosan (PC) with different degrees of phosphate contents were synthesized and the effect of phosphate grafting on electrospinning behavior of substituted polymers is investigated. In PC, higher phosphate content widened concentration range for nanofiber formation. Balance between conductivity and viscosity of solutions played a determinant role in the success of electrospinning process. Culture of L929 cells showed grafting-dependent increase in cell proliferation. On the other hand, culture of MG-63 cells showed a positive correlation between grafting degree and Alkaline Phosphatase (ALP) expression. It is concluded that improvement of cell response parameters of nanofiber scaffolds can be attained as a function of controlled degree of phosphate grafting in polymeric biomaterials with implications for bone tissue engineering applications. Such studies may also be useful to develop quantitative structure activity relationships of functional polymers.

Hyaluronic Acid (HA)‐Based Silk Fibroin/Zinc Oxide Core–Shell Electrospun Dressing for Burn Wound Management

“…The morphology of the attached cells on dried electrospun scaffolds was observed under SEM and images were taken. [ 107 ]…”

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

“…After 21 days of culture, a decrease in ALP ratio on CMKC nanofibers is observed, which is consistent with osteoblast activity in the bone matrix, since ALP is expressed at the beginning of the process and its expression declines with further cell development. [64,67,68] The PVA nanofibers exhibit a delayed ALP ratio increase after 21 days of culture.…”

Comparative Study of Physicochemical Properties and Biocompatibility (L929 and MG63 Cells) of TiN Coatings Obtained by Plasma Nitriding and Thin Film Deposition

“…The MG63 cell line was chosen because it is also recommended for studying bone tissue engineering. 39 To obtain an extraction solution, we put the DMEM culture medium in contact with all the samples at a 3 cm 2 •mL −1 concentration for 24 h at 37 °C in 5% CO 2 .…”

“…The L929 cells were used in this study because they possess high correlation with results of animal bioassays (they are approved by ISO biocompatibility procedures). The MG63 cell line was chosen because it is also recommended for studying bone tissue engineering . To obtain an extraction solution, we put the DMEM culture medium in contact with all the samples at a 3 cm 2 ·mL –1 concentration for 24 h at 37 °C in 5% CO 2 .…”