Hyaluronic Acid (HA)‐Based Silk Fibroin/Zinc Oxide Core–Shell Electrospun Dressing for Burn Wound Management

Abstract

Burn injuries represent a major life‐threatening event that impacts the quality of life of patients, and places enormous demands on the global healthcare systems. This study introduces the fabrication and characterization of a novel wound dressing made of core–shell hyaluronic acid–silk fibroin/zinc oxide (ZO) nanofibers for treatment of burn injuries. The core–shell configuration enables loading ZO—an antibacterial agent—in the core of nanofibers, which in return improves the sustained release of the drug and maintains its bioactivity. Successful formation of core–shell nanofibers and loading of zinc oxide are confirmed by transmission electron microscopy, Fourier‐transform infrared spectroscopy, and energy dispersive X‐ray. The antibacterial activity of the dressings are examined against Escherichia coli and Staphylococcus aureus and it is shown that addition of ZO improves the antibacterial property of the dressing in a dose‐dependent fashion. However, in vitro cytotoxicity studies show that high concentration of ZO (>3 wt%) is toxic to the cells. In vivo studies indicate that the wound dressings loaded with ZO (3 wt%) substantially improves the wound healing procedure and significantly reduces the inflammatory response at the wound site. Overall, the dressing introduced herein holds great promise for the management of burn injuries.

Bio-Based Electrospun Fibers for Wound Healing

“…In this case, the sheath protects the unstable biological agent from an aggressive environment and delivers it in a sustained way by minimizing the burst release. Superior mechanical properties, and the possibility to functionalize the surface without affecting the core material are other advantages [6,59,63,64]. Besides the morphological features, the architecture of the wound dressings and arrangement of the fibers in their structure also greatly affect the adhesion, proliferation, and penetration of the cells, and release behavior of the bioactive molecules from the structure.…”

“…In this case, the sheath protects the unstable biological agent from an aggressive environment and delivers it in a sustained way by minimizing the burst release. Superior mechanical properties, and the possibility to functionalize the surface without affecting the core material are other advantages [ 6 , 59 , 63 , 64 ].…”

Recent Trends in Three-Dimensional Bioinks Based on Alginate for Biomedical Applications

“…It is worth noting that we are still a great distance from organ printing. Even though recent deposition and manufacturing systems permit us to develop structures similar to tissue in their composition, the progression of entirely functioning tissue is a much more significant step [ 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 ].…”

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

“…Nevertheless, restricted materials with appropriate printability, stability of construct in SBF and mechanical characteristic continue to be the serious challenges that have to be conquered prior to extensive designing for products manufacturing in biomedical fields. It is worth noting that the electro-spinning approach has gained considerable attention from biomedical experts all around the world for manufacturing various polymers, particularly CS-based polymer scaffolds loaded with drugs for biomedical and wound dressing purposes [ 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 ]. Application of CS-based biopolymers through 3D [ 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 ] printing for biomedical purposes especially as bio-ink in an effort to fabricate complex tissues will probably be an upcoming pattern of 3D printing materials progression.…”