Cellular aspects of the rat uterine IgA response to estradiol and progesterone

Wira, Charles R.; Hyde, Elisabeth; Sandoe, Carola P.; Sullivan, David A.; Spencer, Steve

doi:10.1016/0022-4731(80)90306-4

Cited by 51 publications

(15 citation statements)

References 18 publications

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“…Free sc and slgA are also present in pro-oestrous uterine fluid, suggesting that sc mediates the movement of IgA from the tissue into the uterine lumen (Sullivan & Wira, 1981). Using immunofluorescent labelling, Wira et al (1980) reported the presence of many IgA plasma cells in the endometrium and myometrium during the oestrous cycle, and oestradiol treatment stimulated the appearance of IgA-positive cells in the uteri of ovariectomized rats ). On the other hand, in a preliminary report, Mitchell (1986) concluded that IgA plasma cells were not present in uterine tissue from cyclic rats, but IgA was detected in the luminal and glandular epithelia, predominantly at pro-oestrus and oestrus.…”

Section: Introductionmentioning

confidence: 94%

Immunohistochemical investigation of secretory component and immunoglobulin A in the genital tract of the female rat

Parr

Parr²

1989

Reproduction

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There was intense labelling of secretory component (sc) in the glandular and luminal epithelia of the uterine horns at pro-oestrus, oestrus and Day 1 of pregnancy, but at other stages labelling was weak or undetectable. There was also intense labelling of sc in the superficial layer of cells in the stratified epithelia of the cervix and vagina at pro-oestrus and Days 4-7 of pregnancy, but not at other stages. Plasma cells containing immunoglobulin A (IgA) were not observed in any region of the genital tract at any of the times studied. The presence of sc coupled with an absence of IgA-containing plasma cells suggest that IgA in genital tract secretions of the female rat may be derived mainly from serum.

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Section: Introductionmentioning

confidence: 94%

Immunohistochemical investigation of secretory component and immunoglobulin A in the genital tract of the female rat

Parr

Parr²

1989

Reproduction

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“…However, there is uncertainty regarding the role of progesterone in regulating the local immune system in the uterus. and Wira, Hyde, Sandoe, Sullivan & Spencer (1980) showed that progesterone inhibited the oestradiol-induced accumulation of IgA in the rat uterine lumen and IgA plasma cells in the endometrium. A similar antagonism by progesterone was also observed in the mouse (Canning & Billington, 1983).…”

Section: Introductionmentioning

confidence: 97%

Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract

Parr¹,

Parr²

1985

Reproduction

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The localization of immunoglobulins A, G and M (IgA, IgG, IgM) in the mouse genital tract was studied by immunoperoxidase techniques at oestrus, on the day of mating and at the time of implantation. In the horns and body of the uterus, IgA and IgG were located in plasma cells in the endometrium surrounding uterine glands and in the gland lumina. The numbers of these plasma cells increased markedly between Day 1 and Days 4 and 5 of pregnancy and the ratio of plasma cells containing IgA and IgG was about 3 or 4 to 1 at all stages. Area measurements indicated that the increased number of plasmacytes was not due to an increase in the amount of endometrial, myometrial or glandular tissues. Plasma cells were not detected in the cervix and vaginal fornix at oestrus and Day 1, but a few were present on Day 5. In the oviduct, plasma cells containing IgA and IgG were present only in the preampulla and both immunoglobulins were present in the extracellular space of the lamina propria only in this region. No IgM was detected in any part of the reproductive tract at any of the times studied. Uteri on Day 1 of pregnancy contained bacteria of several kinds, some of which were aggregated and coated with IgA. This suggests that the uterine lumen at this time may contain specific anti-bacterial IgA antibodies.Our observations indicate that the horns and body of the uterus and the preampulla of the oviduct are major sites of a local immune system in the female mouse genital tract.

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“…For example, during the estrous cycle, levels of IgA, IgG, and SC in uterine secretions are higher at proestrus, when estradiol levels in blood are known to be elevated, than at any other stage of the cycle [14]. Estradiol administered to ovariectomized animals regulates the mucosal immune system in the female reproductive tract by controlling IgA and IgG movement from blood to tissue [13], stimulating the movement of IgApositive cells into the uterus [15] and regulating the transfer of IgA from tissue to lumen through increases in pIgR levels [16]. In the latter study, the addition of cycloheximide, actinomycin D, and a-amanitin to the incubation medium decreased SC production in vitro by uteri taken from estradioltreated rats [16].…”

Section: Introductionmentioning

confidence: 99%

Polymeric Immunoglobin (Ig) Receptor Production and IgA Transcytosis in Polarized Primary Cultures of Mature Rat Uterine Epithelial Cells1

Richardson¹,

Kaushic²,

Wira³

1995

Biology of Reproduction

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These studies were conducted to more fully understand the role of uterine epithelial cells (UEC) in immunoglobin (Ig)A movement from tissue into secretions in the female reproductive tract. Indirect immunofluorescence and image analysis showed that the polymeric Ig receptor (pIgR), which is responsible for transporting polymeric IgA (pIgA) across epithelial cells, was expressed in uterine tissues from rats throughout the estrous cycle. UEC pIgR levels were higher at estrus than at either proestrus or diestrus. When UEC were isolated from the uteri of adult rats and grown on cell culture inserts, cells grew to confluence, formed tight junctions, and released secretory component (SC), the external domain of the pIgR, into the apical medium. Irrespective of whether UEC were isolated from the uteri of rats at the diestrous, proestrous, or estrous stages of the reproductive cycle, cells produced SC, indicating that they are capable of IgA transport. 125I-IgA was preferentially transcytosed from the basolateral to the apical surface, demonstrating that dimeric IgA (dIgA) could be transported by UEC in culture. In contrast, the fluid phase marker [3H]inulin moved at a comparable rate in both directions across the cell monolayer. 125I-IgA transport through UEC was saturable and specific for pIgA in that unlabeled pIgA, but not IgG, inhibited 125I-dIgA transcytosis from the basolateral to the apical surface. Immunoprecipitation of 125I-IgA in the apical chamber with rabbit anti-SC antibody indicated that after transepithelial movement, IgA was bound to SC. Northern blot analysis of RNA extracted from UEC demonstrated that cells continued to synthesize pIgR mRNA in culture. Our results suggest that in the uterus, epithelial cells play a key regulatory role in the control of IgA transcytosis from tissue into secretions.

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Cellular aspects of the rat uterine IgA response to estradiol and progesterone

Cited by 51 publications

References 18 publications

Immunohistochemical investigation of secretory component and immunoglobulin A in the genital tract of the female rat

Immunohistochemical investigation of secretory component and immunoglobulin A in the genital tract of the female rat

Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract

Polymeric Immunoglobin (Ig) Receptor Production and IgA Transcytosis in Polarized Primary Cultures of Mature Rat Uterine Epithelial Cells1

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