Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract

Parr, Margaret B.; Parr, Earl L.

doi:10.1530/jrf.0.0740361

Cited by 65 publications

(47 citation statements)

References 25 publications

(51 reference statements)

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“…Presumably, anti-HSV-2 serum responses were generated within the GLNs and by the dissemination of plasmablasts to distant sites (McDermott & Bienenstock, 1979;McDermott et al, 1980). Moreover, smaller antibody responses in vaginal washings compared to sera may reflect the small numbers of plasmacytes present in the murine genital tract (McDermott & Bienenstock, 1979;McDermott et al, 1980;Parr & Parr, 1985). Studies to identify the anatomical locations of HSV-2-specific plasma cells are in progress.…”

Section: Discussionmentioning

confidence: 99%

“…tt is not known why IgG was the predominant anti-HSV-2 isotype measured in vaginal washings although it was likely not to be of serum origin. IgG-containing plasmacytes are found in the genital mucosa of mice (McDermott & Bienenstock, 1979;McDermott et al, 1980;Parr & Parr, 1985) and, certainly, the local production of antigen-specific IgA and IgG antibodies secreted into cervico-vagina! fluids of women with genital HSV-2 (Mendis et al, 1981;Merrinran et al, 1984) or Chlamydia trachomatis infections (Brunham et al, 1983;Levitt & Barol, 1987;Richmond et al, 1980) suggests that both of these isotypes are important in controlling the magnitude and duration of these sexually transmitted diseases.…”

Section: Discussionmentioning

confidence: 99%

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Mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2

McDermott

Brais

Evelegh

1990

Journal of General Virology

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Herpes simplex virus type 2 (HSV-2) causes lethal illness after intravaginal (IVAG) inoculation into BALB/cJ mice. In the present studies, we demonstrated in mice that primary IVAG vaccination with an attenuated strain of HSV-2 induced humoral immunity in sera and in vaginal secretions, Secondary genital exposure to HSV-2 enhanced this response. However, intraperitoneal exposure to attenuated HSV-2 elicited an antiviral antibody response in sera but not in vaginal secretions. In both sera and vaginal secretions, antiviral IgG antibodies were the major isotype. Systemic exposure to HSV-2 elicited antibodies only in sera that were specific for the major viral antigens whereas IVAG inoculation with HSV-2 stimulatedboth serum and vaginal antibody responses. Intravenous transfer of antiviral monoclonal antibodies protected against systemic HSV-2 infection but were ineffective against vaginal infection due to a lack of transudation into vaginal secretions. These results suggested that local humoral immunity in the genital tract is important in resistance to HSV-2.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2

McDermott

Brais

Evelegh

1990

Journal of General Virology

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“…11 However, IgG and IgA plasma cells have been reported in the reproductive tract of female mice 12,13 and IgA plasma cells together with a few IgG plasma cells have been described in the human fallopian tube. 14 In rabbits, an early study showed that T cells, together with a few IgA plasma cells and macrophages, are present in the endometrium.…”

Section: Introductionmentioning

confidence: 99%

Lymphocytes and MHC class II positive cells in the female rabbit reproductive tract before and after ovulation

Janssens

Holland

et al. 2005

Immunol Cell Biol

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SummaryIn this study, we identified lymphocytes and MHC class II positive (MHC-II+) cells in the reproductive tract of female rabbits both before and after ovulation. CD43+ T cells were frequently present in the mucosa of the oviduct, cervix, and vagina, but far fewer positive cells were seen in the endometrium. The induction of ovulation did not change the cell density in these regions. KEN-5+ T cells and MHC-II+ cells were also frequently seen in the mucosa of the oviduct, cervix, and vagina both before and after ovulation. However, in the uterus, there were very few positive cells before ovulation, but the number increased dramatically after ovulation. Associated with the increase of KEN-5+ T cells, IL-2 mRNA expression in the uterus also increased after ovulation, suggesting that the uterus experienced an increase of T-cell activation. IgM-and IgA-positive B cells were not commonly seen in the reproductive tract and the induction of ovulation did not alter this. Our results suggest that the reproductive tract of female rabbits has the capacity to mount an immune response and that the immune cell distribution of the rabbit reproductive tract has some distinctive features compared with that found in other species.

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“…In the present study, we found that both IgG-and IgA-secreting hybridoma cells migrated from peripheral blood to male and female genital tracts, and their location in normal male and female genital tracts has no great difference from that of normal ISC reported in humans and rats by other studies. 4,7,8,25 In other words, ISC in genital tracts of normal individuals can be supplied by circulating ISC. The RDI of IgG-and IgA-secreting cells in all examined genital tracts had no significant difference between two cells of IgG and IgA isotypes.…”

Section: Disscusionmentioning

confidence: 99%

“…5,7 In mice, IgA and IgG plasma cells but not IgM plasma cells are found in horn and body of uterus, oviduct, fornix of vagina, cervix, and urethral glands. 8,9 The resident ISC may be locally developed from antigencommitted B cells provided by inductive sites. In the presence of specific antigen or signals (such as CD40L), some mature B cells are activated in situ, proliferate to form foci, and then differentiate into ISC (plasma cells).…”

Section: Introductionmentioning

confidence: 99%

Circulating immunoglobulin A‐ and immunoglobulin G‐secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones

et al. 2002

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SUMMARYAntigen-specific circulating immunoglobulin-secreting cells (ISC) migrate to various secondary and tertiary lymphoid tissues. To understand the migration of the cells into the genital tract and its regulation by sex hormones, spleen-derived SG2 hybridoma cells secreting immunoglobulin G2b (IgG2b) and Peyer's patch-derived PA4 hybridoma cells secreting polymer IgA were labelled with 3 H-TdR, and intravenously injected into syngeneic mice of both sexes. Using flow cytometry, surface molecular markers of plasma cells, CD38 and CD138, and adhesion molecules, CD49d, CD162, and CD11a were found to be positive in SG2 and PA4 cells, but CD62L, a4b7 and CD44 were not expressed on these cells. The relative distribution indexes (RDIs) of the cells in genital tract and other tissues were measured. The means of RDIs of SG2 and PA4 cells in female genital tissues were 6 . 5 and 4 . 5 times as many as the means in male genital tissues, respectively. The treatment of ovariectomized mice with b-oestradiol significantly increased the RDIs of PA4 cells in cervix and vagina, but decreased the RDIs of SG2 cells in vagina, horn of uterus, uterus and rectum (P<0 . 05). Progesterone treatment increased the RDIs of PA4 cells in vagina and rectum (P<0 . 05). The treatment with testosterone significantly increased the RDIs of SG2 and PA4 cells in epididymis and accessory sex glands (P<0 . 05). These results demonstrate that the female genital tract is the preferable site for the migration of circulating hybridoma cells to the male genital tract, and sex hormones play an important role in regulation of the migration of circulating ISC to genital tracts.

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Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract

Cited by 65 publications

References 25 publications

Mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2

Mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2

Lymphocytes and MHC class II positive cells in the female rabbit reproductive tract before and after ovulation

Circulating immunoglobulin A‐ and immunoglobulin G‐secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones

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