Cell Wall Characteristics and Structure of Hydrated and Dry leaves of the Resurrection Plant Craterostigma wilmsii, a Microscopical Study

Vicré, Maïté; Sherwin, Heather W.; Driouich, Azeddine; Jaffer, Mohamed A.; Farrant, Jill M.

doi:10.1016/s0176-1617(99)80088-1

Cited by 79 publications

(86 citation statements)

References 26 publications

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“…Analysis of the alkali-insoluble pectin showed a marked increase in GalUA content upon desiccation. This is interesting because it correlates with an immunocytochemical study of desiccation-induced changes in the cell wall of the resurrection plant C. wilmsii, which showed that pectin epitopes increased upon desiccation (Vicré et al, 1999). A further biochemical study of the cell wall of C. wilmsii revealed no overall change in GalUA content between states, but did reveal a change in solubility of pectin polymers as determined by differential fractionation from hydrated and desiccated cell walls (Vicré et al, 2004b).…”

Section: Discussionsupporting

confidence: 53%

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Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius

et al. 2006

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The Myrothamnus flabellifolius leaf cell wall and its response to desiccation were investigated using electron microscopic, biochemical, and immunocytochemical techniques. Electron microscopy revealed desiccation-induced cell wall folding in the majority of mesophyll and epidermal cells. Thick-walled vascular tissue and sclerenchymous ribs did not fold and supported the surrounding tissue, thereby limiting the extent of leaf shrinkage and allowing leaf morphology to be rapidly regained upon rehydration. Isolated cell walls from hydrated and desiccated M. flabellifolius leaves were fractionated into their constituent polymers and the resulting fractions were analyzed for monosaccharide content. Significant differences between hydrated and desiccated states were observed in the water-soluble buffer extract, pectin fractions, and the arabinogalactan protein-rich extract. A marked increase in galacturonic acid was found in the alkali-insoluble pectic fraction. Xyloglucan structure was analyzed and shown to be of the standard dicotyledonous pattern. Immunocytochemical analysis determined the cellular location of the various epitopes associated with cell wall components, including pectin, xyloglucan, and arabinogalactan proteins, in hydrated and desiccated leaf tissue. The most striking observation was a constitutively present high concentration of arabinose, which was associated with pectin, presumably in the form of arabinan polymers. We propose that the arabinan-rich leaf cell wall of M. flabellifolius possesses the necessary structural properties to be able to undergo repeated periods of desiccation and rehydration.

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Section: Discussionsupporting

confidence: 53%

“…The monoclonal antibody JIM 13 has been shown to recognize polysaccharide epitopes of AGPs (Knox et al, 1991;Yates et al, 1996;Knox, 1997). Immunocytochemistry was performed essentially as described previously (Vicré et al, 1999).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius

et al. 2006

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“…Nevertheless this characteristic may be associated with the prevention of mechanical damages in plasmalema during reduced water availability, as suggested by Vicré et al (1999) for tissues of desiccation tolerant seeds.…”

Section: Discussionmentioning

confidence: 94%

Leaf anatomy of two Anemia Sw. species (Schizaeaceae-Pteridophyte) from a rocky outcrop in Niterói, Rio de Janeiro, Brazil

Ribeiro¹,

Santos²,

Moraes³

2007

Rev. bras. Bot.

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-(Leaf anatomy of two Anemia Sw. species (Schizaeaceae-Pteridophyte) from a rocky outcrop in Niterói, Rio de Janeiro, Brazil). The ferns Anemia tomentosa (Sav.) Sw. var. anthriscifolia (Schrad.) Mickel and Anemia villosa Humb. & Bonpl. ex Willd. are widely associated with vegetation islands on rocky outcrops in Rio de Janeiro. Both species are desiccation tolerant. The leaf anatomy of these species was examined aiming to identify morphological characteristics that would allow the establishment of these species in water-scarce environments. The plants were harvested on "Pedra de Itacoatiara" and prepared according to the usual procedures. The petiole has a uniseriate epidermis with lignified cell walls, conical stegmata, and uniseriate multicelular and glandular trichomes.

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“…This may be due to other eco-physiological changes in the leaves resulting from the drying process, which can alter, for example, the internal structure of cells, and the chlorophyll and carotenoid content of leaves [48]. Cell wall architecture of dry leaves has been studied microscopically, where they found the cell walls to contract during the dehydration process [49]. There could be a relation between the contraction of cells and backscattered reflectance if this process is modifying the interaction between the laser beam and the leaf surface.…”

Section: Discussionmentioning

confidence: 99%

Measuring Leaf Water Content with Dual-Wavelength Intensity Data from Terrestrial Laser Scanners

et al. 2016

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Decreased leaf moisture content, typically measured as equivalent water thickness (EWT), is an early signal of tree stress caused by drought, disease, or pest insects. We investigated the use of two terrestrial laser scanners (TLSs) employing different wavelengths for improving the understanding how EWT can be retrieved in a laboratory setting. Two wavelengths were examined for normalizing the effects of varying leaf structure and geometry on the measured intensity. The relationship between laser intensity features, using red (690 nm) and shortwave infrared (1550 nm) wavelengths, and the EWT of individual leaves or groups of needles were determined with and without intensity corrections. To account for wrinkles and curvatures of the leaves and needles, a model describing the relationship between incidence angle and backscattered intensity was applied. Additionally, a reflectance model describing both diffuse and specular reflectance was employed to remove the fraction of specular reflectance from backscattered intensity. A strong correlation (R 2 = 0.93, RMSE = 0.004 g/cm 2 ) was found between a normalized ratio of the two wavelengths and the measured EWT of samples. The applied intensity correction methods did not significantly improve the results of the study. The backscattered intensity responded to changes in EWT but more investigations are needed to test the suitability of TLSs to retrieve EWT in a forest environment.

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Cell Wall Characteristics and Structure of Hydrated and Dry leaves of the Resurrection Plant Craterostigma wilmsii, a Microscopical Study

Cited by 79 publications

References 26 publications

Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius

Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius

Leaf anatomy of two Anemia Sw. species (Schizaeaceae-Pteridophyte) from a rocky outcrop in Niterói, Rio de Janeiro, Brazil

Measuring Leaf Water Content with Dual-Wavelength Intensity Data from Terrestrial Laser Scanners

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