Decreased leaf moisture content, typically measured as equivalent water thickness (EWT), is an early signal of tree stress caused by drought, disease, or pest insects. We investigated the use of two terrestrial laser scanners (TLSs) employing different wavelengths for improving the understanding how EWT can be retrieved in a laboratory setting. Two wavelengths were examined for normalizing the effects of varying leaf structure and geometry on the measured intensity. The relationship between laser intensity features, using red (690 nm) and shortwave infrared (1550 nm) wavelengths, and the EWT of individual leaves or groups of needles were determined with and without intensity corrections. To account for wrinkles and curvatures of the leaves and needles, a model describing the relationship between incidence angle and backscattered intensity was applied. Additionally, a reflectance model describing both diffuse and specular reflectance was employed to remove the fraction of specular reflectance from backscattered intensity. A strong correlation (R 2 = 0.93, RMSE = 0.004 g/cm 2 ) was found between a normalized ratio of the two wavelengths and the measured EWT of samples. The applied intensity correction methods did not significantly improve the results of the study. The backscattered intensity responded to changes in EWT but more investigations are needed to test the suitability of TLSs to retrieve EWT in a forest environment.

No abstract

Norway spruce is one of the most important commercial forestry species in Europe, and is commonly infected by the bark beetle-vectored necrotrophic fungus, Endoconidiophora polonica. Spruce trees display a restricted capacity to respond to environmental perturbations, and we hypothesized that water limitation will increase disease severity in this pathosystem. To test this prediction, 737 seedlings were randomized to high (W+) or low (W−) water availability treatment groups, and experimentally inoculated with one of three E. polonica strains or mock-inoculated. Seedling mortality was monitored throughout an annual growing season, and total seedling growth and lesion length indices were measured at the experiment conclusion. Seedling growth was greater in the W+ than W− treatment group, demonstrating limitation due to water availability. For seedlings infected with two of the fungal strains, no differences in disease severity occurred in response to water availability. For the third fungal strain, however, greater disease severity (mortality and lesion lengths) occurred in W− than W+ seedlings. While the co-circulation in nature of multiple E. polonica strains of varying virulence is known, this is the first experimental evidence that water availability can alter strain-specific disease severity.

Changing climate is increasing the amount and intensity of forest stress agents, such as drought, pest insects, and pathogens. Leaf water content, measured here in terms of equivalent water thickness (EWT), is an early indicator of tree stress that provides timely information about the health status of forests. Multispectral terrestrial laser scanning (MS-TLS) measures target geometry and reflectance simultaneously, providing spatially explicit reflectance information at several wavelengths. EWT and leaf internal structure affect leaf reflectance in the shortwave infrared region that can be used to predict EWT with MS-TLS. A second wavelength that is sensitive to leaf internal structure but not affected by EWT can be used to normalize leaf internal effects on the shortwave infrared region and improve the prediction of EWT. Here we investigated the relationship between EWT and laser intensity features using multisensor MS-TLS at 690, 905, and 1,550 nm wavelengths with both drought-treated and Endoconidiophora polonica inoculated Norway spruce seedlings to better understand how MS-TLS measurements can explain variation in EWT. In our study, a normalized ratio of two wavelengths at 905 and 1,550 nm and length of seedling explained 91% of the variation (R2) in EWT as the respective prediction accuracy for EWT was 0.003 g/cm2 in greenhouse conditions. The relation between EWT and the normalized ratio of 905 and 1,550 nm wavelengths did not seem sensitive to a decreased point density of the MS-TLS data. Based on our results, different EWTs in Norway spruce seedlings show different spectral responses when measured using MS-TLS. These results can be further used when developing EWT monitoring for improving forest health assessments.

Forests are the largest terrestrial ecosystem covering one third of the earth's surface area (Roxburgh and Noble, 2013), and they provide a range of services such as carbon uptake (Hardiman et al., 2011), productivity (Puettmann et al., 2015), biodiversity (Fedrowitz et al., 2014), and resilience (Messier et al., 2013). Processes of growth and regeneration are closely related to these services also linking them with forest structure (von Gadow et al., 2012). The current forest structure is a result of tree and stand dynamics affected

Sustainable tree resource management is the key to mitigating climate warming, fostering a green economy, and protecting valuable habitats. Detailed knowledge about tree resources is a prerequisite for such management but is conventionally based on plot-scale data, which often neglects trees outside forests. Here, we present a deep learning-based framework that provides location, crown area and height for individual overstory trees from aerial images at country scale. We apply the framework on data covering Denmark and show that large trees (stem diameter > 10 cm) can be identified with a low bias (12.5%), and that trees outside forests contribute to 30% of the total tree cover, which is typically unrecognized in national inventories. The bias is high (46.6%) when our results are evaluated against all trees taller than 1.3 m, which involve undetectable small or understory trees. Furthermore, we demonstrate that only marginal effort is needed to transfer our framework to data from Finland, despite markedly dissimilar data sources. Our work lays the foundation for digitalized national databases, where large trees are spatially traceable and manageable.

No abstract

Terrestrial laser scanning (TLS) has been adopted as a feasible technique to digitize trees and forest stands, providing accurate information on tree and forest structural attributes. However, there is limited understanding on how a variety of forest structural changes can be quantified using TLS in boreal forest conditions. In this study, we assessed the accuracy and feasibility of TLS in quantifying changes in the structure of boreal forests. We collected TLS data and field reference from 37 sample plots in 2014 (T1) and 2019 (T2). Tree stems typically have planar, vertical, and cylindrical characteristics in a point cloud, and thus we applied surface normal filtering, point cloud clustering, and RANSAC-cylinder filtering to identify these geometries and to characterize trees and forest stands at both time points. The results strengthened the existing knowledge that TLS has the capacity to characterize trees and forest stands in space and showed that TLS could characterize structural changes in time in boreal forest conditions. Root-mean-square-errors (RMSEs) in the estimates for changes in the tree attributes were 0.99–1.22 cm for diameter at breast height (Δdbh), 44.14–55.49 cm2 for basal area (Δg), and 1.91–4.85 m for tree height (Δh). In general, tree attributes were estimated more accurately for Scots pine trees, followed by Norway spruce and broadleaved trees. At the forest stand level, an RMSE of 0.60–1.13 cm was recorded for changes in basal area-weighted mean diameter (ΔDg), 0.81–2.26 m for changes in basal area-weighted mean height (ΔHg), 1.40–2.34 m2/ha for changes in mean basal area (ΔG), and 74–193 n/ha for changes in the number of trees per hectare (ΔTPH). The plot-level accuracy was higher in Scots pine-dominated sample plots than in Norway spruce-dominated and mixed-species sample plots. TLS-derived tree and forest structural attributes at time points T1 and T2 differed significantly from each other (p < 0.05). If there was an increase or decrease in dbh, g, h, height of the crown base, crown ratio, Dg, Hg, or G recorded in the field, a similar outcome was achieved by using TLS. Our results provided new information on the feasibility of TLS for the purposes of forest ecosystem growth monitoring.