Cell viability analysis of Toxocara cati larvae with LIVE / DEAD® Viability/Cytotoxicity kit

Sena-Lopes, Ângela; Remião, Mariana H.; Alves, Mirna Samara Dié; Fonseca, Bárbara da Rocha; Seixas, Fabiana Kömmling; Collares, Tiago; Borsuk, Sibele

doi:10.1016/j.exppara.2020.107871

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Cells viability was assessed using CCK-8 and the live/dead viability/cytotoxicity kit, where RSC96 cells were cultured on samples for 24 h and then co-cultured with 10 mM H 2 O 2 for 8 h. For the quantitative assay, NSCs viability was evaluated by CCK-8 as described above. For the qualitative assay, cytotoxicity was analyzed using the live/dead viability/cytotoxicity kit, according to the manufacture’s protocol (Sena-Lopes et al 2020 ). Briefly, cells were washed with PBS followed by the addition of 2 µM Calcein AM and 4 µM ethidium homodimer (EthD-1).…”

Section: Methodsmentioning

confidence: 99%

Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress

Mao

Du²,

Zhu

et al. 2022

J Mol Histol

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Functionalizing biomaterial substrates with biological signals shows promise in regulating cell behaviors through mimicking cellular microenvironment. Calcium phosphate (CaP) coating is an excellent carrier for immobilizing biological molecules due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. In this study, we reported the adhesion, the viability and proliferation behaviors after oxidative stress injury of Schwann cells RSC96 on CaP immobilized with the Velvet Antler Peptide (VAP) isolated from velvet antler through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing VAP. This approach provided well retention of functional molecules up to 28 days, and supported the adhesion and proliferation of RSC96 after oxidative stress injury without cytotoxicity. The simple and reproducible method of coprecipitation suggests that CaP is an ideal carrier to functionalize materials with biological molecules for peripheral nerve repair-related applications.

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Section: Methodsmentioning

confidence: 99%

Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress

Mao

Du²,

Zhu

et al. 2022

J Mol Histol

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“…Cytotoxicity was evaluated using the LIVE/DEAD staining kit (L3224, Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions [ 62 ]. Briefly, 0.2 g specimen was immersed in 1 mL culture medium at 37 °C for 24 h, and the medium was used as the extracted medium for subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

Modified Low-Temperature Extraction Method for Isolation of Bletilla striata Polysaccharide as Antioxidant for the Prevention of Alzheimer’s Disease

Lin

Fang

Liang

et al. 2021

IJMS

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Amyloid-β (Aβ) peptides play a key role in Alzheimer’s disease (AD), the most common type of dementia. In this study, a polysaccharide from Bletilla striata (BSP), with strong antioxidant and anti-inflammatory properties, was extracted using a low-temperature method and tested for its efficacy against AD, in vitro using N2a and BV-2 cells, and in vivo using an AD rat model. The characterization of the extracted BSP for its molecular structure and functional groups demonstrated the effectiveness of the modified method for retaining its bioactivity. In vitro, BSP reduced by 20% reactive oxygen species (ROS) levels in N2a cells (p = 0.0082) and the expression levels of inflammation-related genes by 3-fold TNF-α (p = 0.0048), 4-fold IL-6 (p = 0.0019), and 2.5-fold IL-10 (p = 0.0212) in BV-2 cells treated with Aβ fibrils. In vivo, BSP recovered learning memory, ameliorated morphological damage in the hippocampus and cortex, and reduced the expression of the β-secretase protein in AlCl3-induced AD rats. Collectively, these findings demonstrated the efficacy of BSP for preventing and alleviating the effects of AD.

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“…Cell viability and morphology of MCF-7 cells were analyzed after drug exposure using the LIVE/DEAD assay (Biotium Hayward, CA, USA) and fluorescence microscopy (FLM). 17,18 After a 24-h drug exposure (0, 10, and 100 µM CPC, 500 µM DOX) and a triple wash with PBS (pH 7.4), cells were incubated in the dark for 0.5 h at 37°C with 100 µL live/dead staining suspension. Finally, cells were observed with an EVOS Cell Imaging System (Thermo Fisher Scientific, CA, USA) using FITC and rhodamine filters.…”

Section: Live/dead Assaymentioning

confidence: 99%

Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way

García-Cuéllar

Hernandez-Delgadillo

Solís-Soto

et al. 2022

Journal of Applied Biomaterials & Functional Materials

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Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity.

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Cell viability analysis of Toxocara cati larvae with LIVE / DEAD® Viability/Cytotoxicity kit

Cited by 5 publications

References 13 publications

Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress

Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress

Modified Low-Temperature Extraction Method for Isolation of Bletilla striata Polysaccharide as Antioxidant for the Prevention of Alzheimer’s Disease

Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way

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