Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress

Abstract

Functionalizing biomaterial substrates with biological signals shows promise in regulating cell behaviors through mimicking cellular microenvironment. Calcium phosphate (CaP) coating is an excellent carrier for immobilizing biological molecules due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. In this study, we reported the adhesion, the viability and proliferation behaviors after oxidative stress injury of Schwann cells RSC96 on CaP immobilized with the Velvet Antler Peptide (VAP) isolated from velvet antler through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing VAP. This approach provided well retention of functional molecules up to 28 days, and supported the adhesion and proliferation of RSC96 after oxidative stress injury without cytotoxicity. The simple and reproducible method of coprecipitation suggests that CaP is an ideal carrier to functionalize materials with biological molecules for peripheral nerve repair-related applications.

Facile construction of calcium titanate-loaded silk fibroin scaffolds hybrid frameworks for accelerating neuronal cell growth in peripheral nerve regeneration

“…According to the literature and with certain adjustments made in this investigation [ 30 ], the hybridized scaffolds with known weight (Wk) were soaked in ethanol for at least 10 min under vacuum conditions (0.08 MPa) to remove the air bubbles. After that, the hybridized scaffolds were removed, and the excess ethanol was absorbed rapidly using filter paper.…”

“…It has been studied extensively for its structural, electric, and optical properties because of its versatility in structural transformations and applications in ferroelectric and dielectric ceramic materials, high-performance capacitors, sensors, and electroluminescent devices [ 29 ]. Due to its negative temperature coefficient is also known as an impressively chemically resistant n-type semiconductor, a ceramic dielectric with a high dielectric constant, and a thermal resistive element [ 30 ]. Nano- and micron-sized CaTiO 3 particles, films, and composites have also received significant research and development attention.…”