Cell substrates for the production of viral vaccines

Aubrit, Françoise; Perugi, Fabien; Léon, Arnaud; Guéhenneux, Fabienne; Champion-Arnaud, Patrick; Lahmar, Mehdi; Schwamborn, Klaus

doi:10.1016/j.vaccine.2015.06.110

Cited by 55 publications

(90 citation statements)

References 70 publications

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“…In contrast, the MOI is a critical parameter when optimizing the production of lytic viruses used for the synthesis of recombinant proteins. Theoretically, in the best-case scenario, the termination of protein synthesis would coincide with medium depletion just prior to cell lysis [71]. This is also necessary during the production of lytic therapeutic viruses in order to recover active virus particles.…”

Section: Multiplicity Of Infectionmentioning

confidence: 99%

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Grein¹,

Weidner²,

Czermak³

2017

New Insights Into Cell Culture Technology

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The industrial-scale manufacturing of viruses or virus-like particles in cell culture is necessary for gene therapy and the treatment of cancer with oncolytic viruses. Complex multistep processes are required in both cases, but the low virus titers in batch cultures and the temperature sensitivity of the virus particles limit the production scale. To meet commercial and regulatory requirements, each process must be scalable and reproducible and must yield high virus titers. These requirements are met by establishing a cell culture process that matches the properties of the virus/host-cell system and by using serum-free cell culture medium. This chapter focuses on two case studies to consider the different aspects of process design, such as the reactor configuration and operational mode: the continuous production of retroviral pseudotype vectors in a retroviral packaging cell line and the production of oncolytic measles virus vectors for cancer therapy.

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Section: Multiplicity Of Infectionmentioning

confidence: 99%

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Grein¹,

Weidner²,

Czermak³

2017

New Insights Into Cell Culture Technology

View full text Add to dashboard Cite

show abstract

“…The manufacturing of whole-cell vaccines, based initially on viruses grown in vivo in whole animals or in ovo in embryonated eggs, is now moving towards animal cell culture systems allowing virus propagation in vitro. Chicken embryo fibroblast (CEF) primary cells, human lung-derived Medical Research Council 5 (MRC5) diploid cells, the African green monkey kidney-derived (Vero) and Madin Darby canine kidney (MDCK) cells (continuous cell lines) are the most common cell substrates currently used for vaccine production [5,19]. VLPs can be produced in a variety of cell culture systems including microbial (bacteria and yeasts, mainly Escherichia coli and Saccharomyces cerevisiae), insect (e.g., High Five™ cells from Trichoplusiani used in the Baculovirus Expression Vector System), mammalian (e.g., Chinese hamster ovary (CHO) cells) and -to a lesser extent-plant cells [16,17].…”

Section: Viral Particle Purificationmentioning

confidence: 99%

“…(c) The production of safe and effective influenza vaccines, either egg-based or cellbased, is a major goal of the pharmaceutical industry [123][124][125]. While most currently licensed influenza vaccines are derived from embryonated eggs [124,125], cell culture technology [19] is an emerging approach in influenza vaccine manufacturing [123][124][125]. MDCK and, to a lesser extent, Vero are the main cell lines used for influenza vaccine production [123].…”

Section: Pseudo-affinity Chromatography (Pafc)mentioning

confidence: 99%

Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

Junter

Lebrun

2020

Journal of Pharmaceutical Analysis

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“…As características desses tipos de vacinas apresentados no Quadro 3 devem ser levadas em consideração ao se pensar em fazer uma implementação em escala industrial. Essa opinião é compartilhada por Astleyet al (2007) e Aubrit et al (2015). O segundo autor afirmou, ainda, que nenhuma linhagem celular, nem mesmo as projetadas, é capaz de preencher todos os critérios para todas as vacinas virais.…”

Section: O Processo De Imunizaçãounclassified

PRODUÇÃO DE VACINAS VIRAIS PARTE I: engenharia de bioprocessos

Bousada

Pereira²

2017

RUVRV

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RESUMOAs vacinas são uma das maiores conquistas da humanidade em termos de diminuição das doenças. O correto entendimento de sua produção, a partir não só dos conhecimentos biológicos, mas também em termos de engenharia, é de fundamental importância para que os avanços já adquiridos possam ir cada vez mais longe. A produção de vacinas torna-se, assim, um importante nicho de pesquisa para os engenheiros, de modo especial na produção de vacinas virais, destacadas no presente artigo, em que o cultivo de células animais deve estar alinhado com as questões de biossegurança e bioética. Como estudo de caso é apresentada a produção da vacina contra o influenza no Instituto Butantan, no Brasil. A segunda parte do artigo discute, sob a perspectiva da bioética personalista ontologicamente fundada, sobre a eticidade do uso de linhagens celulares derivadas de abortos, tais como a MRC-5, WI-38, PER.C6 e HEK293, na produção de algumas vacinas. PALAVRAS-CHAVE: Vacinas virais. Engenharia de bioprocessos. Escalonamento. Biossegurança. Bioética. ABSTRACTVaccines are one of the most important achievements of humankind in terms of protection against diseases. The understanding of their production, considering not only biological issues, but also the engineering perspective, is of core importance for the continuous improvement in this field. The Bioprocess Engineering aproach conveys an important area for engineer survey, particularly for virus vaccines, described in more detail in this article, where animal cells cultivation is deeply related with economics, bio-safety and bioethical issues.As a study case, this article also presents the influenza vaccine production by Butantan Institute, in Brazil. The second part of this article discusses, under ontologically founded personalist bioethics perspective, about the use of cell cultures derived from abortions, such as MRC-5, WI-38, PER.C6 e HEK293, in the production of some vaccines.

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Cell substrates for the production of viral vaccines

Cited by 55 publications

References 70 publications

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

PRODUÇÃO DE VACINAS VIRAIS PARTE I: engenharia de bioprocessos

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