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The synthesis and phytotoxic activity of a series of tyrosol 1,2,3-triazole derivatives are reported herein. Target compounds were synthesized through the copper­(I)-catalyzed azide-alkyne cycloaddition reaction (CuAAC), known as click reaction, and these were tested for phytotoxic activity on leaves of wild poinsettia (Euphorbia heterophylla), fleabane (Conyza sumatrensis), and tropical spiderwort (Commelina benghalensis). These are three highly noxious agricultural weeds that challenge available weed control methods, including the use of chemical herbicides. Twenty-five compounds were synthesized and tested. None of the compounds showed phytotoxic activity against C. benghalensis and C. sumatrensis, but almost all of them produced yellowing, bleaching, and necrosis on leaves of E. heterophylla. Two of the tyrosol 1,2,3-triazole derivatives produced more extensive lesions than those produced by the commercial herbicide diquat, used as a positive control (p ≤ 0.05). When applied on leaves of E. heterophylla, these compounds interfered with the stomatal conductance, net photosynthesis, internal carbon concentration, transpiration rate, water-use efficiency, and chlorophyll A and B contents. The interference of such compounds on such photosynthesis-related variables indicates that tyrosol 1,2,3-triazole derivatives may be capable of lowering the competitiveness of E. heterophylla and acting as additional tools for managing this competitive weed in agricultural lands.

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RESUMOAs vacinas são uma das maiores conquistas da humanidade em termos de diminuição das doenças. O correto entendimento de sua produção, a partir não só dos conhecimentos biológicos, mas também em termos de engenharia, é de fundamental importância para que os avanços já adquiridos possam ir cada vez mais longe. A produção de vacinas torna-se, assim, um importante nicho de pesquisa para os engenheiros, de modo especial na produção de vacinas virais, destacadas no presente artigo, em que o cultivo de células animais deve estar alinhado com as questões de biossegurança e bioética. Como estudo de caso é apresentada a produção da vacina contra o influenza no Instituto Butantan, no Brasil. A segunda parte do artigo discute, sob a perspectiva da bioética personalista ontologicamente fundada, sobre a eticidade do uso de linhagens celulares derivadas de abortos, tais como a MRC-5, WI-38, PER.C6 e HEK293, na produção de algumas vacinas. PALAVRAS-CHAVE: Vacinas virais. Engenharia de bioprocessos. Escalonamento. Biossegurança. Bioética. ABSTRACTVaccines are one of the most important achievements of humankind in terms of protection against diseases. The understanding of their production, considering not only biological issues, but also the engineering perspective, is of core importance for the continuous improvement in this field. The Bioprocess Engineering aproach conveys an important area for engineer survey, particularly for virus vaccines, described in more detail in this article, where animal cells cultivation is deeply related with economics, bio-safety and bioethical issues.As a study case, this article also presents the influenza vaccine production by Butantan Institute, in Brazil. The second part of this article discusses, under ontologically founded personalist bioethics perspective, about the use of cell cultures derived from abortions, such as MRC-5, WI-38, PER.C6 e HEK293, in the production of some vaccines.