Cell proliferation assay for determination of estrogenic components in food: a systematic review

Abstract

Due to the widespread use and environmental pollution of estrogenic chemicals, the need for screening tests to detect these compounds is felt more than ever. These compounds lead to cell proliferation. Therefore, studies used cell proliferation to evaluate estrogenic compounds was studied in this systematic review. This systematic review was performed with the keywords; DNA proliferation, cell proliferation, estrogenic component, estrogen, food, bioassay, screening, and detection. After initial screening and full text quality assessment, 16 manuscripts were selected and data were extracted. Four cell lines, MCF-7, MDA-MB-231, Ishikawa, and T47D cells were used in the studies. MCF-7 was more sensitive to estrogenic compounds than other lines. Most of the samples studied were plant compounds and mycotoxins and substances that migrate from packaging to food. This screening test is valid and has similar results as others.

Bisphenol A in dairy products, amount, potential risks, and the various analytical methods, a systematic review

“…Therefore, one of the safety evaluations of these products in terms of BPA is to determine HQ and EDI. HQ (Hazard Quotient) is to consider non-carcinogenic risks ( Sadighara and Ghanati, 2022 , Sadighara et al, 2022 ). The EDI in Mercogliano/2021 study was calculated for different age groups, which was higher in children than in adults ( Mercogliano et al, 2021 ).…”

“…Regarding compounds that interfere with endocrine glands, bisphenol A has the most attention and also the most danger ( Herrero, Quintanilla-López, Fernández, & Gómara, 2021 ). This compound binds to estrogen receptors ( Cai et al, 2018 , Sadighara et al, 2022 , Sadighara and Ghanati, 2022 ). This compound can synergize with other xenoestrogens in the body and have adverse health effects ( Grumetto et al, 2013 ).…”