Cell-imprinted substrates: in search of nanotopographical fingerprints that guide stem cell differentiation

Kamguyan, Khorshid; Moghaddam, Saeed Zajforoushan; Nazbar, Abolfazl; Haramshahi, Seyyed Mohammad Amin; Taheri, Shiva; Bonakdar, Shahin; Thormann, Esben

doi:10.1039/d0na00692k

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…They analyzed the substrates by AFM, obtained roughness profiles at the nanoscale, and confirmed the difference between the nanotopographies of the cellular imprints. 65 These findings can guarantee the usage of the PDMS imprinting method to copy and save the molecular pattern of the cell membrane precisely for future clinical regulation of cell behaviors.…”

Section: Resultsmentioning

confidence: 89%

Molecular imprinting as a simple way for the long-term maintenance of the stemness and proliferation potential of adipose-derived stem cells: an in vitro study

et al. 2022

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Section: Resultsmentioning

confidence: 89%

Molecular imprinting as a simple way for the long-term maintenance of the stemness and proliferation potential of adipose-derived stem cells: an in vitro study

et al. 2022

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“…The cell-imprinted biointerfaces can record the complementary geometry of the templated cells, spanning from the macroscale cell shape to the nanoscale antibody. 42 In nature, a variety of topographical structures with nano-and microscale hierarchy are optimized to have specialized functions. As shown in Figure 2a, the myocardial cells in the native heart tissue were well-aligned and close-packed.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Cell imprinting is an outstanding technology for creating customized morphologies, resulting in complementary morphological and chemical fitting structures from the templating cells. , Moreover, the cell-imprinted materials might maintain the natural extracellular residues, which might facilitate cell adhesion, differentiation, and growth. Typically, the cell phenotypical functions can be influenced by a multitude of complex stimuli, such as biochemical, structural, and physical signals. ,,,, Contributing to the matched physical shapes and complementary chemical compositions, the cell-imprinted substrates have been applied to educate the differentiation of stem cells and improve the detecting sensitivity of circulating tumor cells, and so on.…”

Section: Introductionmentioning

confidence: 99%

“…Typically, the cell phenotypical functions can be influenced by a multitude of complex stimuli, such as biochemical, structural, and physical signals. 5,9,29,40,41 Contributing to the matched physical shapes and complementary chemical compositions, the cell-imprinted substrates have been applied to educate the differentiation of stem cells 42 and improve the detecting sensitivity of circulating tumor cells, 43 and so on.…”

Section: ■ Introductionmentioning

confidence: 99%

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Natural Tissue-Imprinted Biointerface for the Topographical Education of a Biomimetic Cell Sheet

Yao¹,

Meng³

et al. 2022

Langmuir

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Cell sheet engineering as a cell-based scaffold-free therapy is promising in tissue engineering, allowing precise transforming treatments for various tissue damage. However, the current cutting-edge techniques are still hampered by the difficulty in mimicking the natural tissue organizations and the corresponding physiological functions. In this work, cell-imprinting technology using the natural tissue as a template was proposed to rationally educate the cellular alignment in the cell sheet. Through this technique, we obtained temporary templates with morphological structure complementary to native tissues and then directly transferred the structure on the template to the collagen layer on a photothermally convertible substrate by secondary imprinting replication. The resultant biomimetic interface was used for cell culture and release to obtain a cell sheet with a texture similar to the natural tissue morphology. Different from conventional photolithography, the natural tissue-imprinted biointerface guides the geometry of cell sheets in the way of natural principles instead of stereotyped or overuniform cell organization. Simultaneously, a near-infrared laser (NIR) was used to irradiate the photothermally responsive substrate to obtain complete cell sheets efficiently and nondestructively. The natural tissue-educated myocardium cell sheets exhibited good physiological activity and biomimetic biofunctions, such as mechanical properties and physiological performances. This approach might open an inspiring prospect in regenerative medicine and offer a new approach to realizing the biomimetic tissue construction.

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“…The regulation of cell adhesion and growth is significant in microscale tissue engineering, biosensor design, applied cell biology, and high-flux cell screening [74][75][76]. Cell imprinting is also an effective strategy to modulate cell adhesion and growth [77]. For example, McNaughton and co-workers observed the promoted adhesion and growth of HeLa cells on cell imprinted polyacrylamide hydrogels [78].…”

Section: Whole-cell Imprintingmentioning

confidence: 99%

Selective recognition of tumor cells by molecularly imprinted polymers

Gao

et al. 2021

J of Separation Science

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Molecularly imprinted polymers, developed 50 years ago, have garnered enormous attention as receptor-like materials. Lately, molecularly imprinted polymers have been employed as a specific target tool in favor of cancer diagnosis and therapy by the selective recognition of tumor cells. Although the molecular imprinting technology has been well-innovated recently, the cell still remains the most challenging target for imprinting. In this review, we summarize the advances in the synthesis of molecularly imprinted polymers suitable for the selective recognition of tumor cells. Through a sustained effort, three strategies have been developed including peptide-imprinting, polysaccharide-imprinting, and whole-cell imprinting, which have resulted in inspiring applications in effective cancer diagnosis and therapy. The major challenges and perspectives on the further directions related to the synthesis of molecularly imprinted polymers were also outlined.

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Cell-imprinted substrates: in search of nanotopographical fingerprints that guide stem cell differentiation

Abstract: Cell-imprinted substrates direct stem cell differentiation into various lineages, suggesting lineage-specific nanotopography that is studied herein by an extensive AFM roughness analysis.

Cited by 5 publications

References 37 publications

Molecular imprinting as a simple way for the long-term maintenance of the stemness and proliferation potential of adipose-derived stem cells: an in vitro study

Molecular imprinting as a simple way for the long-term maintenance of the stemness and proliferation potential of adipose-derived stem cells: an in vitro study

Natural Tissue-Imprinted Biointerface for the Topographical Education of a Biomimetic Cell Sheet

Selective recognition of tumor cells by molecularly imprinted polymers

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