Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals

A total of 211 Acinetobacter strains (representing all currently recognized genomic species) were tested for 329 biochemical characters. Overall similarities of all strains were determined for 145 characters by numerical taxonomic techniques, the UPGMA algorithm and the S(SM)) and the S(J) coefficients as measures of similarity. Seven clusters (two or more strains) and three unclustered strains were recovered at a similarity level of 80.0% (S(SM). At this level a complete correspondence between phenotypic cluster and genomic species was found only for genomic species 12 (Ac. radioresistens). At higher similarity levels (84.0% to 84.6% (S(SM)), however, several subclusters were found, each representing a single genomic species. An exception were the strains belonging to the genetically closely related species of the Acinetobacter calcoaceticus-baumannii complex. These were recovered scattered in several subclusters. The degree of genomic relatedness between some DNA groups correlated with phenotypic similarities, especially for DNA group 8 (Ac. Iwoffii) and 15 of Tjernberg and Ursing, and for DNA group 4 (Ac. haemolyticus) and 6. For the majority of genomic species, two identification matrices were constructed consisting of 22 and 10 diagnostic characters, respectively. The correct identification rates for the matrices were 98.0% (22 tests) and 90.8% (10 tests) taking a Willcox probability > 0.9. For unambiguous identification of some genomic species, however, additional methods (preferably DNA-DNA hybridization or ribotyping) should be used.

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“…T h e use of additional methods such as cell envelope protein profiles (CEPPs) (Dijkshoorn et a(. 1987a(Dijkshoorn et a(.…”

Section: Discussionmentioning

confidence: 99%

Numerical classification and identification ofAcinetobactergenomic species

Kämpfer

Tjernberg

Ursing

1993

Journal of Applied Bacteriology

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“…FO-1 and A. calcoaceticus ATCC 2305524 and A. calcoaceticus 69V. 25 The cell envelope profiles of the six clinical isolates in this study did not exhibit the marked heterogeneity reported by Dijkshoorn et al 26 in their study of 78 clinical isolates. The OMP profiles appear similar to the "pattern D" established by these workers.…”

Section: Discussionmentioning

confidence: 48%

Immune response to Acinetobacter calcoaceticus infection in man

Smith¹,

Alpar²

1991

Journal of Medical Microbiology

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Summary.After growth in an iron-depleted chemically-defined medium Acinetobacter calcoaceticus expressed four high mol. wt outer-membrane proteins (OMPs) which were repressed under iron supplementation or in a complex laboratory medium. Immunoblotting with serum from a septicaemic patient infected with A. calcoaceticus revealed antibody binding to these iron-repressible OMPs, indicating that they were expressed in vivo, and also to the 42-and 18-Kda OMPs. Although the antibody response to the OMPs did not vary significantly during convalescence, the response to the O-polysaccharide component of lipopolysaccharide decreased significantly. However, antibodies in serum from patients with A. calcoaceticus wound infections reacted with the iron repressible OMPs and a 54-Kda antigen suggesting a difference in immune recognition between local and systemic infection.

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“…For this reason, great importance is attached to the recognition and elimination of sources of infection and routes of transmission by analysis of epidemiological data based on the fine differentiation of the agents involved. In the case of Acinetobacter spp., this has been more or less successfully accomplished using serotyping [3], biotyping [4], phage-typing [5], determination of resistance to antibiotics [6], examination of cell envelope protein profiles [7] and other methods.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and clonal relationships ofAcinetobacter baumanniistrains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis

Thurm

Ritter

1993

Epidemiol. Infect.

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SUMMARYSixty-five strains of Acinetobacter baumannii which had been isolated from patients and the indoor environment of a neonatal intensive care unit and, for comparative purposes, isolates from three other wards, were examined by means of electrotyping and analysis of whole-cell protein and antibiotic resistance patterns. Fourteen different electrotypes were determined. The predominant type, a multiply resistant acinetobacter clone, persisted in the neonatal ward over several months. The results underline the usefulness of electrophoretic subtyping, in particular by means of allozyme pattern and as a supplement to whole-cell protein pattern analysis, in epidemiological investigations into the routes of transmission of nosocomial A. baumannii infections.

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Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals

Cited by 55 publications

References 22 publications

Numerical classification and identification ofAcinetobactergenomic species

Numerical classification and identification ofAcinetobactergenomic species

Immune response to Acinetobacter calcoaceticus infection in man

Genetic diversity and clonal relationships ofAcinetobacter baumanniistrains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis

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