Genetic diversity and clonal relationships of<i>Acinetobacter baumannii</i>strains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis

Thurm, V.; Ritter, Erich

doi:10.1017/s0950268800057228

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…Investigation of 27 esterases and 2 dehydrogenases in 81 Acinetobacter isolates identified between 2 and 17 variants for each enzyme (150). A separate study of 13 enzymes in 65 Acinetobacter clinical isolates identified 14 different types, of which 1 type was found in 41 multiresistant isolates with common whole-cell protein profiles (193). These results suggest that multilocus enzyme electrophoresis has the potential to be developed into a useful technique for strain identification.…”

Section: Multilocus Enzyme Electrophoretic Typingmentioning

confidence: 86%

Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features

1996

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Section: Multilocus Enzyme Electrophoretic Typingmentioning

confidence: 86%

Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features

1996

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“…Other researchers compared protein typing by SDS‐PAGE and allotyping for selected bacterial enzymes as a means to track isolates in epidemiological investigations. Although allotyping had the greatest discriminating power, protein analysis was concluded to be more widely applicable between diverse bacterial systems 24, 25. Not all of the comparative studies of the protein profiles used detailed cluster analysis for their comparisons and manual comparisons were still widely used 24.…”

Section: Typing Bacteria Using 1‐d Sds‐pagementioning

confidence: 99%

Proteomics in the study of the molecular taxonomy and epidemiology of bacterial pathogens

Cash

2009

Electrophoresis

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The ability to discriminate bacterial isolates is important for a number of areas of research in Medical Microbiology, particularly in defining bacterial taxonomy and monitoring transmission in epidemiological investigations. Molecular techniques capable of typing bacteria at the level of the genome and proteome are now widely used for these investigations. This review considers two electrophoretic methods for typing bacteria on the basis of their proteomes, namely 1-D SDS-PAGE and 2-DE. The application of these two techniques for bacterial typing is described with reference to two publications that appeared in Electrophoresis [Costa, Electrophoresis 1990, 11, 382-391 and Cash et al., Electrophoresis 1997, 18, 1472-1482]. Even though these methods have been used for nearly 20 years to differentiate bacterial isolates they remain key technologies in proteome-based typing methods. The developments that have arisen from the two key papers are described in order to highlight the advantages and disadvantages in typing bacteria at the level of their proteomes. Some of the difficulties associated with electrophoretic typing methods can be overcome by using non-gel proteomic methods based on MS to provide improved sensitivity and specificity. The application of proteomic methods to investigate bacterial taxonomy, epidemiology and pathogenesis in general has significant potential in furthering our understanding of infectious diseases.

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“…For several years, the prevalence of infection with MDR A. baumannii has been increasing dramatically in the European countries that is, from 15.4% in 2004 to 48.5% in 2014 [8]. In the late 20th century, A. baumannii has emerged as a significant nosocomial pathogen in Germany [9][10][11]. Numerous nosocomial outbreaks were reported among inpatients and preterm infants with a considerable mortality rate of up to 24% after bacteremia in intensive care units at a large teaching hospital of Cologne [12].…”

Section: Introductionmentioning

confidence: 99%

Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)

et al. 2020

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Acinetobacter (A.) baumannii has gained global notoriety as a significant nosocomial pathogen because it is frequently associated with multi-drug resistance and hospital-based outbreaks. There is a substantial difference in the incidence of A. baumannii infections between different countries and within Germany. However, its continuous spread within Germany is a matter of concern. A systematic literature search and analysis of the literature published between 2000 and 2018 on A. baumannii in humans was performed. Forty-four studies out of 216 articles met the criteria for inclusion, and were selected and reviewed. The number of published articles is increasing over time gradually. Case reports and outbreak investigations are representing the main body of publications. North Rhine-Westphalia, Hesse and Baden-Wuerttemberg were states with frequent reports. Hospitals in Cologne and Frankfurt were often mentioned as specialized institutions. Multiresistant strains carrying diverse resistance genes were isolated in 13 of the 16 German states. The oxacillinase blaOXA-23-like, intrinsic blaOXA-51-like, blaOXA-58 variant, blaNDM-1, blaGES-11, blaCTX-M and blaTEM are the most predominant resistance traits found in German A. baumannii isolates. Five clonal lineages IC-2, IC-7, IC-1, IC-4 and IC-6 and six sequence types ST22, ST53, ST195, ST218, ST944/ST78 and ST348/ST2 have been reported. Due to multidrug resistance, colistin, tigecycline, aminoglycosides, fosfomycin, ceftazidime/avibactam and ceftolozan/tazobactam were often reported to be the only effective antibiotics left to treat quadruple multi-resistant Gram-negative (4MRGN) A. baumannii. Dissemination and infection rates of A. baumannii are on the rise nationwide. Hence, several aspects of resistance development and pathogenesis are not fully understood yet. Increased awareness, extensive study of mechanisms of resistance and development of alternative strategies for treatment are required. One-Health genomic surveillance is needed to understand the dynamics of spread, to identify the main reservoirs and routes of transmission and to develop targeted intervention strategies.

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Genetic diversity and clonal relationships ofAcinetobacter baumanniistrains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis

Cited by 15 publications

References 14 publications

Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features

Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features

Proteomics in the study of the molecular taxonomy and epidemiology of bacterial pathogens

Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)

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