Cell cycle inhibition of potato root tips treated with Imazethapyr

Abstract

Imazethapyr inhibits the progression of the cell cycle in potato (Solanum tubemsum L.) root tips. A variety of existing methods were developed and adapted for the determination of mitotic index in potatoes. Significantly lower numbers of mitotically dividing cells were recorded after just 30 min of incubation with imazethapyr (0.35 p~) and the effect was characterised as an inhibition of mitotic entry. Imazethapyr inhibits a key enzyme in the biosynthesis of the branched-chain amino acids, valine, leucine and isoleucine. The inhibition of mitotic entry can be considered to be a secondary manifestation of a primary metabolic change induced by imazethapyr.

Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay

“…Zhang et al [33] suggested that application of the herbicide IM in soybean fields changed the soil microbial biomass and shifted the microbial community structure. Solanum tuberosum [35], Vicia faba [36] and Triticum durum [37]. Sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinylthio (or oxy)-benzoates and sulfonylaminocarbonyltriazolinones are AHAS-inhibiting herbicides [38][39][40].…”

Toxicidade da mistura formulada de imazethapyr e imazapic sobre o azevém em função do teor de umidade do solo

“…A redução de estatura de plantas após a aplicação desse herbicida é sintoma comumente observado em várias culturas, como o arroz irrigado (Fontana et al, 2007;Marchesan et al, 2010), o azevém (Pinto et al, 2009a), o milho (Pinto et al, 2009c) e o sorgo (Pinto et al, 2009b). A redução da estatura de plantas ocorre provavelmente porque esses herbicidas, além de inibirem a enzima ALS, como um efeito secundário, inibem a divisão celular dos pontos de crescimento das plantas, como observado no sistema radicular de batata (Spackman & Cobb, 1999). ) presente no solo na toxicidade dos herbicidas sobre as plantas de azevém avaliadas aos 49 dias após a emergência das plantas.…”

A simple method for identification of S phase nuclei in Vicia faba root meristems using BrdUrd labeling and indirect immunofluorescence (comparison with 3H-thymidine incorporation)

“…Hence, evaluation of cell cycle progression in root apices still provides a vehicle for much reasearch in cell population kinetics, having revelance and applicability for both pure science and agriculture (see Yim andBayer 1996, Spackman andCobb 1999).…”