Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides

Choi, Bong‐Hyuk; Choi, Yoo Seong; Kang, Dong Gyun; Kim, Bum Jin; Song, Young Hoon; Joon, Hyung

doi:10.1016/j.biomaterials.2010.08.027

Cited by 73 publications

(55 citation statements)

References 55 publications

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“…To investigate the morphology of osteoblast cells on the DBBM surface, 50 mg of mMAP per cm 2 of well area was coated to the DBBM particle using the precipitation method as followed in a previous study. 21 Then, osteoblast MC3T3-E1 cells (RIKEN Cell Bank, Tsukuba, Japan) were seeded on DBBM alone and on mMAP-coated DBBM samples. Aer 1 day of cultivation under serum-containing conditions, the morphology of the osteoblast cells on the DBBM surfaces was analyzed by scanning electron microscopy (SEM; JSM-6010LV; JEOL Ltd., Tokyo, Japan) using 5 kV secondary electron imaging.…”

Section: Nbt Stainingmentioning

confidence: 99%

“…In total, 50 mg of MAP per cm 2 of well area was used for surface coating, and the coating method was performed according to a previous study. 21 A surface coated with PLL (Sigma-Aldrich) was used as a comparative control, and an uncoated, bare surface was used as a negative control. PLL was coated onto culture plates as described in the manufacturer's instructions.…”

Section: In Vitro Cell Attachment and Proliferation Analysesmentioning

confidence: 99%

“…15,[21][22][23] For in vitro DOPA modication, 2 g L À1 of puried original MAP was dissolved in 200 mM acetate buffer (pH 5.5) with 20 mM ascorbic acid and 20 mM boric acid. Mushroom tyrosinase (Sigma-Aldrich, St. Louis, MO) was then added to the solution to a nal concentration of 50 mg L…”

Section: Preparation Of Mmapmentioning

confidence: 99%

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Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

et al. 2015

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Section: Nbt Stainingmentioning

confidence: 99%

Section: In Vitro Cell Attachment and Proliferation Analysesmentioning

confidence: 99%

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Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

et al. 2015

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“…[220] In addition to functionalizing materials with cell-or biomolecule-specific binding properties, proteins with broad (or almost no) binding specificity are also used for materials functionalization. Extremely adhesive proteins from unusual sources, such as mussel [221] and barnacle [222] adhesive cements, can effectively coat a wide variety of material surfaces for further modification with, e.g., antibacterial peptides. Many of these novel glue-like proteins are effective underwater and have relatively low immunogenicities, [223] further favoring their use in biomaterials applications.…”

Section: Progress Reportmentioning

confidence: 99%

Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.

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“…Dopamine (3,4-dihydroxyphenethylamine), which has a similar structure, has been used by many researchers as a surface treatment to prepare organic layers, even on inorganic surfaces. [13][14][15][16][17][18][19][20] Further, dopamine has been attached to synthetic polymers, derivatives of which are used for surface modification of metals. [21][22][23] These treatments not only provide organic layers, but also increase immobilization of biological molecules on the surfaces of various materials.…”

Section: Introductionmentioning

confidence: 99%

Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

Yang¹,

Zhu²,

Tada³

et al. 2014

IJN

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Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by 1 H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment.

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Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides

Cited by 73 publications

References 55 publications

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

Adding Functions to Biomaterial Surfaces through Protein Incorporation

Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

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