Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

Choi, Bong‐Hyuk; Cheong, Hogyun; Ahn, Jin-Soo; Zhou, Cong; Kwon, Jong Jin; Joon, Hyung; Jun, Sang Ho

doi:10.1039/c4tb01197j

Cited by 20 publications

(18 citation statements)

References 42 publications

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“…The carboxylic acid group and the hydroxyl groups from the catechol of DOPA form ionic bonding with calcium, which seems to indicate that DOPA can bind to bone tissue . The authors claimed that DOPA promoted the process of bone formation as evidenced by the in vivo maturation of new bones with a similar bone density to the normal bone and the in vitro osteogenic differentiation of osteoblast cells …”

Section: Bone Adhesives Made From Natural Polymersmentioning

confidence: 99%

Bone‐Adhesive Materials: Clinical Requirements, Mechanisms of Action, and Future Perspective

Sanchez-Fernandez

Hammoudeh

Lanao

et al. 2019

Adv Materials Inter

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Each year, millions of people suffer from complex bone fractures which require proper external or internal fixation. This fixation is usually achieved by means of devices such as plates, pins, and screws. These traditional fixation strategies are associated with severe drawbacks, which have prompted research and development of a variety of bone‐adhesive biomaterials as alternative. However, a clinically applicable bone‐adhesive biomaterial—in the form of a bone‐glue or bone‐adhesive membrane—that meets all requirements has not yet been identified. This perspective article discusses the current state of the art of bone‐adhesive materials with a particular focus on their clinical requirements, mechanisms of action, and future perspective. To develop adhesive biomaterials with specific affinity to bone tissue, a more rational design should be implemented. This perspective article is intended as a starting point and inspiration for future research and development of suitable bone‐adhesive materials.

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Section: Bone Adhesives Made From Natural Polymersmentioning

confidence: 99%

Bone‐Adhesive Materials: Clinical Requirements, Mechanisms of Action, and Future Perspective

Sanchez-Fernandez

Hammoudeh

Lanao

et al. 2019

Adv Materials Inter

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“…Simple dipping into 1 mg mL −1 m fp‐5 solution at pH 8.5 (50 × 10 −3 m sodium phosphate buffer) also enabled the efficient surface coating of a variety of metal substrates such as aluminum, copper, iron, and stainless steel . Moreover, unmodified fp‐1 and fp‐151 were successfully coated onto polystyrene well surfaces via inducing both MAP aggregation and efficient adsorption in alkaline buffer conditions …”

Section: Surface Engineering With Recombinant Map‐based Coatingsmentioning

confidence: 99%

Biomimetic Surface Engineering of Biomaterials by Using Recombinant Mussel Adhesive Proteins

Kim

Jeong

et al. 2018

Adv Materials Inter

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Surface engineering is a key approach for tailoring new functionalities into biomaterials to achieve better clinical performance. The rise of genetic engineering and molecular biotechnology made it possible to design artificial sticky proteins derived from marine mussels that are capable of firmly anchoring on a variety of substrates with high binding strength, opening a new route for surface engineering of biomaterials. Coatings of recombinant mussel adhesive proteins (MAPs) have aroused great interest for the surface functionalization of biomaterials due to their simplicity, versatility, and high stability under physiological conditions, as well as their favorable interactions with cells. In addition, recombinant MAPs can be engineered to provide desired specific functionalities on target surfaces by genetic fusion with functional peptides and/or by the immobilization of biomolecules. This review provides an overview of recombinant MAPs‐based surface coatings, highlighting their mechanisms, characteristic surface properties, and diverse applications in providing bioengineered surfaces in the fields of biomedical and tissue engineering.

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“…Many different approaches have been proposed in order to solve the problem of wet adhesion. One of the most remarkable has been the study of natural mussel adhesive proteins (MAPS) from the adhesive plaques of Mytilus holdfast or byssus [13], due to their superior properties including biocompatibility, biodegradability and surface-independent adhesiveness even in a wet environment [14]. Over the last decades, several studies have attributed the MAPS coating and adhesive properties to the abundance of l-3,4-dihydroxyphenylalanine (l-DOPA); a catecholamine whose formation is derived from the hydroxylation of the aromatic ring of tyrosine residues [15].…”

Section: Introductionmentioning

confidence: 99%

Specific chemical incorporation of l-DOPA and functionalized l-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired bioadhesives

et al. 2020

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Mussel adhesives proteins have been extensively studied as a promising alternative in bioadhesives due to their ability to provide durable anchoring under different surfaces in seawater. These charasteristics have been attributed to the presence of the reduced catechol form, 3,4-dihydroxyphenylalanine (DOPA) of its structure. However, its practical applications have been limited due to drawbacks with natural extraction. Here, a novel method have been described for site-specific chemical incorporation of l-3,4-dihydroxyphenylalanine methyl ester (l-DOPAME) into proteins, in particular Candida antartica fraction B (CAL-B) lipase. Two strategies were followed, direct conjugation of DOPA at the C-terminus on the surface of the protein, and protein conjugation with tailor-made glycopolymers (DOPA-hyaluronic acid (HA) polymers) at the N-terminus. In all cases, the characterization of the new DOPA-proteins was carried out using circular dichroism, fluorescence or mass spectrometry. An improvement in the activity (in some cases more than 2 times) or the thermostability of CAL-B (with a half live 4 fold greater in some cases) was found by the incorporation of DOPA molecules. These DOPA-proteins showed excellent underwater covalent adhesive ability on amino functionalized surfaces in aqueous media compared to other modified [e.g. tyrosine modified (TYR)] CAL-B proteins. At pH 8.5, CALB-DOPA proteins were completely adsorbed after 90 min of incubation, whereas about 10% of CALB-HA or CALB-TYR proteins were adsorbed at the same time. Native CAL-B adsorption was not observed. These results suggest a potential application of these DOPA-proteins as bioglues or bioadhesives for practical underwater applications.

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Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

Abstract: Engineered mussel bioglue is a promising functional binder for acceleration of bone substitute-assisted bone regeneration with enhanced osteoconductivity and osteoinductivity.

Cited by 20 publications

References 42 publications

Bone‐Adhesive Materials: Clinical Requirements, Mechanisms of Action, and Future Perspective

Bone‐Adhesive Materials: Clinical Requirements, Mechanisms of Action, and Future Perspective

Biomimetic Surface Engineering of Biomaterials by Using Recombinant Mussel Adhesive Proteins

Specific chemical incorporation of l-DOPA and functionalized l-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired bioadhesives

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