Cell and organ printing 1: Protein and cell printers

Wilson, Wesley V.; Boland, Thomas

doi:10.1002/ar.a.10057

Cited by 444 publications

(266 citation statements)

References 14 publications

(9 reference statements)

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“…A description of the printer is given elsewhere (Wilson and Boland, 2003). Sterile stainless steel needles with luer plastic hubs were kept on ice until they were filled with the polymer gel of interest.…”

Section: Cell Printer Preparationmentioning

confidence: 99%

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ABSTRACTWe recently developed a cell printer (Wilson and Boland, 2003) that enables us to place cells in positions that mimic their respective positions in organs. However, this technology was limited to the printing of two-dimensional (2D) tissue constructs.

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mentioning

confidence: 99%

“…We recently developed a cell printer (Wilson and Boland, 2003) that enables us to place cells in positions that mimic their positions in an organ. The printer can put up to nine solutions of cells or polymers into a specific place by the use of specially designed software, and print two-dimensional (2D) tissue constructs.…”

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confidence: 99%

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Cell and organ printing 2: Fusion of cell aggregates in three‐dimensional gels

et al. 2003

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We recently developed a cell printer (Wilson and Boland, 2003) that enables us to place cells in positions that mimic their respective positions in organs. However, this technology was limited to the printing of two-dimensional (2D) tissue constructs. Here we describe the use of thermosensitive gels to generate sequential layers for cell printing. The ability to drop cells on previously printed successive layers provides a real opportunity for the realization of three-dimensional (3D) organ printing. Organ printing will allow us to print complex 3D organs with computer-controlled, exact placing of different cell types, by a process that can be completed in several minutes. To demonstrate the feasibility of this novel technology, we showed that cell aggregates can be placed in the sequential layers of 3D gels close enough for fusion to occur. We estimated the optimum minimal thickness of the gel that can be reproducibly generated by dropping the liquid at room temperature onto a heated substrate. Then we generated cell aggregates with the corresponding (to the minimal thickness of the gel) size to ensure a direct contact between printed cell aggregates during sequential printing cycles. Finally, we demonstrated that these closely-placed cell aggregates could fuse in two types of thermosensitive 3D gels. Taken together, these data strongly support the feasibility of the proposed novel organ-printing technology. Anat Rec Part A 272A: 497-502, 2003.

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Section: Cell Printer Preparationmentioning

confidence: 99%

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Cell and organ printing 2: Fusion of cell aggregates in three‐dimensional gels

et al. 2003

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“…For example, one has adapted commercially available ink-jet print heads into 'cell printers' for the purpose of positioning viable cells onto pre-defined patterns. 4, 5 Ellson performed acoustic droplet ejection of mammalian cells, a method which was shown to be as gentle as a conventional pipette. 6 Santesson et al performed ultrasonic levitation of droplets containing a dozen cells which behaved similarly to non-exposed cells.…”

Section: Introductionmentioning

confidence: 99%

Pumping of mammalian cells with a nozzle-diffuser micropump

et al. 2005

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We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.

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“…Furthermore, an effective bottom-up strategy to generate 3D tissues has been proposed by printing alternate layers of cells and biomaterials into their exact positions thus assembling anatomical structures [6]. Before this strategy can be used the assemble functional neural and cardiac tissues, studies to assess the influence of the printing process on the cells, including the phenotypes and functions of printed neural and cardiac cells must be conducted.…”

Section: Introductionmentioning

confidence: 99%

Fabricating Neural and Cardiomyogenic Stem Cell Structures by a Novel Rapid Prototyping—the Inkjet Printing Method

Gregory

Molnár

et al. 2004

MRS Proc.

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Direct printing of living cardiomyogenic stem cells and embryonic cortical neurons to generate complex cellular patterns and structures of such cells was demonstrated in the study. Furthermore, the immunostaining analyses and the whole-cell patch clamp recordings showed the cortical neurons grown in the printed cellular patterns and structures maintained their basic cellular functions, including neuronal phenotypes and electrophysiological properties. These results and findings may greatly prompt the inkjet printing method evolving into a viable and cost-effective approach for engineering human neural and cardiac tissues or even organs.

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Cell and organ printing 1: Protein and cell printers

Cited by 444 publications

References 14 publications

Cell and organ printing 2: Fusion of cell aggregates in three‐dimensional gels

Cell and organ printing 2: Fusion of cell aggregates in three‐dimensional gels

Pumping of mammalian cells with a nozzle-diffuser micropump

Fabricating Neural and Cardiomyogenic Stem Cell Structures by a Novel Rapid Prototyping—the Inkjet Printing Method

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