Pumping of mammalian cells with a nozzle-diffuser micropump

Yamahata, Christophe; Vandevyver, Caroline; Lacharme, Frédéric; Izewska, Paulina; Vogel, Horst; Freitag, Ruth; Gijs, Martin A. M.

doi:10.1039/b504468e

Cited by 31 publications

(24 citation statements)

References 18 publications

(19 reference statements)

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“…The rapid manufacturing of various microchips with different structures and functions is needed by biologists for observations of different kinds of microorganisms [54][55][56][57]. Meanwhile, micromechanical components such as a microvalve can be integrated in the microfluidics as described above [19].…”

Section: De Nition Of Nanoaquariummentioning

confidence: 99%

Three‐dimensional femtosecond laser micromachining of photosensitive glass for biomicrochips

Sugioka¹,

Hanada

Midorikawa

2010

Laser & Photonics Reviews

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Internal modification of transparent materials such as glass can be carried out using multiphoton absorption induced by a femtosecond (fs) laser. The fs-laser modification followed by thermal treatment and successive chemical wet etching in a hydrofluoric (HF) acid solution forms three-dimensional (3D) hollow microstructures embedded in photosensitive glass. This technique is a powerful method for directly fabricating 3D microfluidic structures inside a photosensitive glass microchip. We used fabricated microchips, referred to as a nanoaquarium, for dynamic observations of living microorganisms. In addition, the present technique can also be used to form microoptical components such as micromirrors and microlenses inside the photosensitive glass, since the fabricated structures have optically flat surfaces. The integration of microfluidics and microoptical components in a single glass chip yields biophotonic microchips, in other words, optofluidics, which provide high sensitivity in absorption and fluorescence measurements of small volumes of liquid samples.Dynamic observation of microorganisms (Euglena gracilis) using nanoaquarium, which has 3D microfluidic structure, fabricated in a photosensitive glass microchip by femtosecond laser direct writing followed by thermal treatment and successive wet chemical etching.

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Section: De Nition Of Nanoaquariummentioning

confidence: 99%

Three‐dimensional femtosecond laser micromachining of photosensitive glass for biomicrochips

Sugioka¹,

Hanada

Midorikawa

2010

Laser & Photonics Reviews

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“…For the precise measurement of the pumping volume, we connected the microtube (inner diameter: 508 μm) at the outlet and measured the distance that the media was moved by the pumping system. Yamahata et al (2005) examined the effect of pulsatile pumping (with nozzle-diffuser micropump) on jurkat cells and 5D10 hybridoma cells. Similarly, we investigated whether the pumping frequency and the pneumatic pumping pressure might affect the cells during delivery to the culture chamber.…”

Section: Measurement Of Pumping Performance and Pumping Effect On Thementioning

confidence: 99%

A cell culturing system that integrates the cell loading function on a single platform and evaluation of the pulsatile pumping effect on cells

Kim

Park

Kwon

et al. 2007

Biomed Microdevices

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In this paper, we present a novel microfluidic system with pulsatile cell storing, cell-delivering and cell culturing functions on a single PDMS platform. For this purpose, we have integrated two reservoirs, a pulsatile pumping system containing two soft check valves, which were fabricated by in situ photopolymerization, six switch valves, and three cell culture chambers all developed through a simple and rapid fabrication process. The sample volume delivered per stroke was 120 nl and the transported volume was linearly related to the pumping frequency. We have investigated the effect of the pulsatile pneumatic micropumping on the cells during transport. For this purpose, we pumped two types of cell suspensions, one containing human breast adenocarcinoma cells (MCF-7) and the other mesenchymal stem cells (hMSCs) derived from bone marrow. The effect of pulsatile pumping on both cell types was examined by short and long-term culture experiments. Our results showed that the characteristics of both cells were maintained; they were not damaged by the pumping system. Evaluations were carried out by morphological inspection, viability assay and immunophenotyping analysis. The delivered MCF-7 cells and hMSCs spread and proliferated onto the gelatin coated cell culture chamber. This total micro cell culture system can be applied to cell-based high throughput screening and for co-culture of different cells with different volume.

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“…Another advantage of our technique based on laser direct writing is that a wide variety of structures can be fabricated by the same procedure with little difficulty, namely, it is suitable for rapid manufacturing. The rapid manufacturing of various microchips with different structures is desired by biologists for the observation of different kinds of cells and microorganisms (Yamahata et al 2005;Choi et al 2007;Haeberle and Zengerle 2007;Funfak et al 2007). In the meanwhile, micromechanical components such as a microvalve can be integrated in the microfluidics ).…”

Section: Introductionmentioning

confidence: 98%

Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass

Hanada

Sugioka

Kawano

et al. 2007

Biomed Microdevices

111

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We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms and cells in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells.

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Pumping of mammalian cells with a nozzle-diffuser micropump

Cited by 31 publications

References 18 publications

Three‐dimensional femtosecond laser micromachining of photosensitive glass for biomicrochips

Three‐dimensional femtosecond laser micromachining of photosensitive glass for biomicrochips

A cell culturing system that integrates the cell loading function on a single platform and evaluation of the pulsatile pumping effect on cells

Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass

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