Cell Adhesion to the Surfaces of Polymeric Beads

Abstract

The main goal of this study is to determine the relationship between the surface properties of polymeric materials and fibroblastic cell adhesion. Therefore, two series of polymeric beads, PHEMA and PS, were tested in microcarrier-facilitated cell culture systems. The crosslinked PHEMA beads were prepared by suspension polymerization of HEMA monomer in the presence of various acrylic monomers (i.e. MMA, EGDMA, DMAEMA). The hydrophobic PS beads were used after coated with different alkylamine monomers (i.e. EDA, ALAM, TEA) by plasma polymerization process. The cell culturing studies were performed with BHK cells in stationary culture conditions and the cell adhesion characteristics were determined by the common methods. Attachment of the BHK cells on these microcarriers were satisfactorily modeled by surface saturation type of mathematical expression. The results demonstrated that, there were finite number of sites on the microcarrier surfaces available for adhesion. Number of these sites depends on the surface charge density which was supplied by amine groups and surface wettability. It is possible to achieve desired cell adhesion and also growth, by changing the chemical structure of beads with suitable modification methods.

Poly(l-glutamic acid)/chitosan polyelectrolyte complex porous microspheres as cell microcarriers for cartilage regeneration

“…This result is in agreement with a study re-620 ported by Yan and co-workers[19]. First, the interaction between 621 carboxyl and amino groups on PLGA and chitosan, respectively, 622 provided the resultant PEC microcarriers with a suitable weakly 623 positively charged surface for attached cell growth[44,45]. Second, 624 the presence of PLGA increased the hydrophilicity of the PEC 625 microspheres, due to the higher hydrophilicity and greater water 626 absorption capability of the residual -COOH groups in PLGA than 627 those of the residual -NH 2 groups in chitosan in the PEC porous 628 microcarriers, thus improving cell attachment and proliferation…”

Microspheres for Enzyme Immobilization

“…The use of microcarrier concentration over 1 g/L was found to result in loss of inoculum, poor growth, and cell detachment (van Wezel, 1977). Kiremitci and Piskin (1990) also reported that the microcarriers significantly inhibited the cell culture when used in large amounts. The optimal charge density varies according to different matrix materials and charge groups.…”

A novel closed system utilizing styrene copolymer bead adherence for the production of human dendritic cells

“…In our initial experiments, we found that the yield of DCs per unit of culture surface area in closed gas‐permeable cell culture bags was less than that in the open‐flask systems. In an effort to improve the yields of DCs in the closed system, we introduced styrene copolymer beads (90‐ to 125‐μ diameter; density, 1.05 g/cm 3 ; SoloHill Engineering, Ann Arbor, MI) into the bags to increase the available surface area (by 380 cm 2 ) and to supply a surface area similar to that found in the flasks 5 . In a biologic safety cabinet, 1 g of gamma‐radiation‐sterilized beads and 10 × 10 8 total cells per bag from the MNC product were diluted in 100 mL of medium (AIM‐V, GIBCO, Grand Island, New York) and loaded into gas‐permeable cell culture bags (Lifecell X‐fold Cell Culture Container PL2417, 180 cm 2 , Nexell Therapeutics, Irvine, CA).…”