Adhesion characteristics of fibroblastic baby hamster kidney (BHK) cells and epithelial Madine Darby kidney (MDBK) cells on polyurethane (PU) and polyhydroxyethylmethacrylate (PHEMA) based surfaces have been studied. PU surfaces were prepared by a classical solvent-casting procedure of Pellethane solution that contains different types of solvents, i.e., THF, dioxane and their compositions. PHEMA based surfaces were obtained by bulk polymerization of respective comonomers (HEMA, acrylic acid, AA, and dimethylaminoethylmethacrylate, DMAEMA) in the presence of the crosslinker. Thus, a number of polymeric surfaces were obtained with different surface charges (COO- and NH+4) and with different surface free energies in a range between 60-82 ergs/cm2. Surface properties of these membranes were characterized by equilibrium water contents, air and octane contact angles, surface free energies, SEM photographs and ATR-FTIR spectra. Interactions of BHK and MDBK cells with the surfaces were examined in stationary culture conditions which were carried out in MEM supplemented with fetal calf serum. The observations strongly suggested that the chemical and/or physical properties of membrane surface and morphology of the cell control the degree of cell adhesion to the PU and PHEMA based membranes.
The main goal of this study is to determine the relationship between the surface properties of polymeric materials and fibroblastic cell adhesion. Therefore, two series of polymeric beads, PHEMA and PS, were tested in microcarrier-facilitated cell culture systems. The crosslinked PHEMA beads were prepared by suspension polymerization of HEMA monomer in the presence of various acrylic monomers (i.e. MMA, EGDMA, DMAEMA). The hydrophobic PS beads were used after coated with different alkylamine monomers (i.e. EDA, ALAM, TEA) by plasma polymerization process. The cell culturing studies were performed with BHK cells in stationary culture conditions and the cell adhesion characteristics were determined by the common methods. Attachment of the BHK cells on these microcarriers were satisfactorily modeled by surface saturation type of mathematical expression. The results demonstrated that, there were finite number of sites on the microcarrier surfaces available for adhesion. Number of these sites depends on the surface charge density which was supplied by amine groups and surface wettability. It is possible to achieve desired cell adhesion and also growth, by changing the chemical structure of beads with suitable modification methods.
Crosslinked poly(hydroxyethylmethacrylate) [poly(HEMA)] beads were prepared by the suspension copolymerization of 2‐hydroxyethylmethacrylate monomer with ethyleneglycol dimethacrylate (EGDMA) in aqueous media which contained only magnesium oxide (MgO) as a suspension stabilizer and also salting‐out agent. The procedure gives spherical particles with narrow size distribution. The reaction mechanism, composition and structure of poly(HEMA) beads were studied by solid‐state 13C CP/MAS NMR, FTIR, ESCA, and elemental analysis. 13C NMR implies the existence of unreacted pendant groups in the HEMA‐EGDMA copolymer. The surface composition obtained by ESCA is almost the same as the bulk composition determined by elemental analysis. FTIR spectra provide compelling evidence for the presence of carboxyl and hydroxyl groups. The glass‐transition temperature and the thermal stability of beads were determined by DSC.
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